A5083925371- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Tissue Engineering and Regenerative Medicine
- Biomedical Ethics and Regulation
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Renal and related cancers
- Energy Harvesting in Wireless Networks
- Cancer Cells and Metastasis
- Immune cells in cancer
- Manufacturing Process and Optimization
- Advanced biosensing and bioanalysis techniques
Thermo Fisher Scientific (United States)
2014-2025
Thermo Fisher Scientific (Israel)
2014-2020
CrossLife Technologies (United States)
2010-2014
Life Systems (United States)
2009-2013
Thermo Fisher Scientific (Norway)
2012
University of Minnesota
2000-2011
Minneapolis VA Medical Center
2007
University of Minnesota System
2007
University of Minnesota Medical Center
1999-2002
Human multipotent mesenchymal stem cell (MSC) therapies are being tested clinically for a variety of disorders, including Crohn's disease, multiple sclerosis, graft-versus-host type 1 diabetes, bone fractures, and cartilage defects. However, despite the remarkable clinical advancements in this field, most applications still use traditional culture media containing fetal bovine serum. The ill-defined highly variable nature remains challenge, hampering both basic human MSC research fields. To...
We provide evidence that the human DNA ligase III gene encodes a mitochondrial form of this enzyme. First, cDNA contains an in-frame ATG located upstream from putative translation initiation start site. The sequence between these two sites amphipathic helix similar to previously identified targeting peptides. Second, recombinant green fluorescent protein harboring at its amino terminus was efficiently targeted mitochondria Cos-1 monkey kidney cells. In contrast, native distributed cytosol....
The ability of embryonic stem cells and adult to differentiate into specific cell types holds immense potential for therapeutic use in gene therapy. Realization this depends on efficient optimized protocols genetic manipulation cells. In the study reported here, we demonstrate nucleofection as a method introduce plasmid DNA with significantly greater efficiency than electroporation or lipid-based transfection methods have. Using enhanced green fluorescent protein (eGFP) reporter gene, mouse...
Many of the currently established human embryonic stem (hES) cell lines have been characterized extensively in terms their gene expression profiles and genetic stability culture. Recent studies indicated that microRNAs (miRNAs), a class noncoding small RNAs participate regulation expression, may play key role self-renewal differentiation. Using both microarrays quantitative PCR, we report here differences miRNA between undifferentiated hES cells corresponding differentiated underwent...
Abstract Human multipotent mesenchymal stem cell (MSC) therapies are currently being tested in clinical trials for Crohn's disease, multiple sclerosis, graft-versus-host type 1 diabetes, bone fractures, cartilage damage, and cardiac diseases. Despite remarkable progress trials, most applications still use traditional culture media containing fetal bovine serum or serum-free that contain albumin, insulin, transferrin. The ill-defined variable nature of remains a challenge has created need...
It has previously been shown that the phage-derived phiC31 integrase can efficiently target native pseudo-attachment sites in genome of various species cultured cells, as well vivo. To demonstrate its utility human embryonic stem cells (hESC), we have created hESC-derived clones containing expression constructs. Variant cell lines BG01v and SA002 were used to derive expressing a green fluorescent protein (GFP) marker under control either Oct4 promoter or EF1alpha promoter. Stable selected by...
Abstract As the number of human embryonic stem cell (hESC) lines increases, so does need for systematic evaluation each line's characteristics and potential. Comparisons between are complicated by variations in culture conditions, feeders, spontaneous differentiation, absence standardized assays. These difficulties, combined with inability most labs to maintain more than a few simultaneously, compel development reference standards which hESC can be compared. The use stable line as standard...
Alkaline phosphatases (AP) are a class of enzymes that hydrolyze phosphate containing molecules under alkaline conditions. In humans, there primarily four different types this enzyme; intestinal, placental, placental-like and non-tissue specific forms. The isozyme AP is expressed in liver, bone kidney. A similar was identified pluripotent stem cells when monoclonal antibodies, TRA-2-49/6E, recognizing determinants human embryonal carcinoma (EC) showed reactivity to isoform.1 also known be at...
DNA end-joining was measured by incubating linearized plasmid with mitochondrial protein extracts. A spectrum of end-joined molecules ranging from re-circularized monomer to dimer and higher molecular weight forms observed. The reaction required ATP Mg2+, inhibited sodium chloride. Both cohesive- blunt-ended were end-joined, although the former more efficient substrates. Molecular analysis rejoined revealed that >95% precisely end-joined. few imprecisely recovered, sustained deletions...
Induced pluripotent stem cells (iPSCs) are promising tools for disease research and cell therapy. One of the critical steps in establishing iPSC lines is early identification fully reprogrammed colonies among unreprogrammed fibroblasts partially intermediates. Currently, colony morphology surface markers used to identify colonies. Through additional clonal characterization, we show that these fail distinguish intermediates from iPSCs. Thus, they can lead selection suboptimal clones...
Hamster EM9 cells, which lack Xrcc1 protein, have reduced levels of DNA ligase III and are defective in nuclear base excision repair. The protein stabilizes may even play a direct role catalyzing Since is also thought to function mitochondrial repair, it seemed likely that would be dependent upon Xrcc1. However, several lines evidence indicate this not the case. First, western blot analysis failed detect extracts. Second, present extracts from cells were indistinguishable those seen similar...
The human DNA ligase III gene encodes both nuclear and mitochondrial proteins. Abundant evidence supports the conclusion that protein plays an essential role in base excision repair homologous recombination. However, of genome dynamics has been obscure. Human tumor-derived HT1080 cells were transfected with antisense expression vector clones diminished levels activity identified. Mitochondrial extracts prepared from these had decreased relative to a control vector. Analysis revealed...