A5011799961- Ovarian cancer diagnosis and treatment
- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Liver physiology and pathology
- Cancer Research and Treatments
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Cancer Genomics and Diagnostics
- Endometrial and Cervical Cancer Treatments
- Drug Transport and Resistance Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Intraperitoneal and Appendiceal Malignancies
- Reproductive Biology and Fertility
- Biomarkers in Disease Mechanisms
- Genetically Modified Organisms Research
- Calcium signaling and nucleotide metabolism
- Renal cell carcinoma treatment
- Cancer Treatment and Pharmacology
- Kruppel-like factors research
- Advanced Breast Cancer Therapies
- Medicinal Plants and Bioactive Compounds
- Cancer Mechanisms and Therapy
- Berberine and alkaloids research
TU Dresden
2017-2025
German Cancer Research Center
2017-2025
University Hospital Carl Gustav Carus
2017-2025
National Center for Tumor Diseases
2017-2025
Heidelberg University
2017-2025
Helmholtz-Zentrum Dresden-Rossendorf
2020-2025
Deutschen Konsortium für Translationale Krebsforschung
2020-2025
University Hospital Heidelberg
2023-2025
German Cancer Society
2024
Klinik für Frauenheilkunde
2021
Environmental chemicals that function as estrogens have been suggested to be associated with an increase in disease and dysfunctions animals humans. To characterize may act humans, we compared three vitro assays which measure aspects of human estrogen receptor (hER)-mediated estrogenicity. Chemicals were first tested for estrogen-associated transcriptional activity the yeast screen (YES). This was created by expressing hER two response elements linked lacZ gene yeast. Second, YES then...
PARP-inhibitors (PARPi) are an integral part of ovarian cancer treatment. However, overcoming acquired PARPi resistance or increasing the benefit in patients without homologous recombination deficiency (HRD) remains unmet clinical need. We sought to identify genetic modulators response, guiding pharmacological sensitization. CRISPR-Cas9 mediated loss-of-function screen with a focused sgRNA library revealed that DNA-demethylases JMJD1B/JMJD1C, targetable by small inhibitor methylstat, promote...
The effect of mixtures environmental chemicals with hormonal activity has not been well studied. To investigate this phenomenon, the estrogen receptor (ER) from American alligator (aER) or human (hER) was incubated [3H]17beta-estradiol in presence selected individually combination. included insecticide chlordane, which no estrogenic activity, and pesticides dieldrin toxaphene, have very weak activity. Chlordane, dieldrin, toxaphene demonstrated appreciable displacement aER hER at...
Colorectal cancers commonly carry truncation mutations in the adenomatous polyposis coli (APC) gene. The APC protein contributes to stabilization of microtubules. Consistently, microtubules cells lacking depolymerize more readily response microtubule-destabilizing drugs. This raises possibility that such agents are suitable for treatment APC-deficient cancers. However, have a compromised spindle assembly checkpoint, which renders them less sensitive killing by microtubule poisons whose...
Abstract Background Leucine-rich α-2 glycoprotein 1 (LRG-1) is a secreted that mainly produced in the liver. Elevated levels of LRG-1 are found multitude pathological conditions including eye diseases, diabetes, infections, autoimmune and cancer. In patients with early breast cancer (BC), high intratumoral protein expression associated reduced survival. this study, we assessed serum BC investigated its correlation presence disseminated tumor cells (DTCs) bone marrow survival outcomes....
The tumour suppressor Adenomatous Polyposis Coli (APC) is required for proper mitosis; however, the exact role of APC in mitosis not understood. Using demembranated sperm chromatin exposed to meiotic Xenopus egg extract and HeLa cells expressing fluorescently labelled histones, we established that contributes compaction. Sperm APC-depleted frequently formed tight round or elongated structures. Such abnormally compacted predominantly spindles with low microtubule content. Furthermore, mitotic...
BACKGROUND Standard biomarker testing of a single macroscopic disease site is unlikely to be sufficient because tumor heterogeneity. A focus on examining global expression or activity, particularly in microscopic residual chemotherapy-resistant disease, needed for the appropriate selection targeted therapies. This study was aimed at establishing technique assessment biomarkers ovarian cancer peritoneal spread. METHODS An in-house developed fluorescent imaging device used detect c-Met...
Overcoming PARPi resistance is a high clinical priority. We established and characterized comparative in vitro models of acquired resistance, derived from either BRCA1-proficient or BRCA1-deficient isogenic background by long-term exposure to olaparib. While parental cell lines already exhibited certain level intrinsic activity multidrug (MDR) proteins, resulting PARPi-resistant cells both further converted toward MDR. In models, the phenotype was shaped (i) cross-resistance other PARPis...
Numerous prognostic models have been proposed for ovarian cancer, extending from single serological factors to complex gene-expression signatures. Nonetheless, these not routinely translated into clinical practice. We constructed a robust and readily calculable model predicting surgical outcome prognosis of cancer patients by exploiting commonly available clinico-pathological three selected serum parameters.
Objectives We have recently described a predictive/prognostic model for ovarian cancer, exploiting commonly available clinico-pathological parameters and the serum biomarkers mesothelin (MSL), human epididymis protein 4 (HE4) cancer-antigen 125 (CA125). Considering urine as prototype non-invasive sample, we investigated whether levels of these are mirrored in compared their clinical relevance matched vs. samples. Methods MSL, HE4 CA125 were quantified urinary (n=172) samples (n=188) from...
The pleiotropic protein hepatocyte growth factor (HGF) is the only known ligand of tyrosine kinase mesenchymal–epithelial transition (cMET) receptor. HGF/cMET pathway mediates invasion and migration ovarian cancer cells, upregulation components has been associated with poor prognosis. This study investigated clinical relevance circulating HGF in serum patients cancer. Serum (sHGF) was determined by enzyme‐linked immunosorbent assay a total 471 samples from 82 healthy controls 113 (88.5% ≥...
Background Neuropilin (NRP) is a transmembrane protein, which has been shown to be pro-angiogenic mediator and implicated as potential driver of cancer progression. NRP-1 up-regulation in ovarian tissue predicts poor prognosis. However, the clinical relevance soluble form (sNRP-1) circulating biomarker patients unknown. Methods/patients cohort sNRP-1 levels were quantified 88 clinically documented by commercially available enzyme-linked immunosorbent assay (ELISA) kit (Biomedica, Vienna,...
// Sarah Schott 1, 2, 3, * , Pauline Wimberger 4, 5, Barbara Klink 6 Konrad Grützmann 5 Julian Puppe 7 Ulrike Sophie Wauer Daniel Martin Klotz Evelin Schröck and Jan Dominik Kuhlmann 1 Department of Gynecology Obstetrics, University Hospital Heidelberg, Germany 2 German Cancer Consortium (DKTK), Dresden Research Center (DKFZ), 3 National for Tumor Diseases (NCT), 4 Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Partner Site Institute Clinical Genetics, Medicine Cologne,...
Abstract Objectives Dickkopf-1 (DKK1) is a secreted protein, known for suppressing the differentiation and activity of bone-building osteoblasts by acting as an inhibitor Wnt-signalling. Soluble DKK1 (sDKK1) has been proposed prognostic biomarker wide range malignancies, however, clinical relevance sDKK1 potential blood-based marker ovarian cancer unknown. Methods levels were quantified in cohort 150 clinically documented patients commercially available ELISA (Biomedica, Vienna, Austria)....
The tyrosine kinase mesenchymal-epithelial transition (cMET) is typically overexpressed in up to 75% of patients with ovarian cancer, and cMET overexpression has been associated poor prognosis. proteolytic release the soluble (sMET) ectodomain by metalloproteases, a process called shedding, reflects malignant potential tumour cells. sMET can be detected human circulation proposed as biomarker several cancers. However, clinical relevance cancer blood-based unknown was therefore investigated...
Creutzfeldt-Jakob disease is a rare and rapidly fatal neurodegenerative disease. Since clinicians may see only very few cases during their professional career, it important to be familiar with the clinical presentation progression, perform appropriate investigations, allow for quick diagnosis.A 73-year-old British Caucasian woman presented acute confusion of 2 weeks' duration on background low mood following recent bereavement. Her symptoms included behavioral change, visual hallucinations,...
e17567 Background: We have recently described a predictive/prognostic model for ovarian cancer, exploiting commonly available clinico-pathological parameters and the serum biomarkers mesothelin (MSL), human epididymis protein 4 (HE4) cancer-antigen 125 (CA125). Considering urine as prototype non-invasive sample, we investigated whether levels of these are mirrored in compared their clinical relevance matched vs. samples. Methods: MSL, HE4 CA125 were quantified by Lumipulse G chemiluminescent...
Abstract Purpose Ovarian cancer is the fifth most common in women and leading cause of death all gynecological malignancies. Prognosis determined by optimal surgical outcome (macroscopic complete resection) commonly achieved tertiary hospitals. We investigated whether versus non-tertiary hospital as location an initial diagnostic intervention for histological confirmation before cytoreductive surgery immediate primary debulking impacts patients with advanced ovarian cancer. Methods analyzed...