A5014539202- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Neuroscience, Education and Cognitive Function
- Neurobiology and Insect Physiology Research
- Congenital gastrointestinal and neural anomalies
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Hedgehog Signaling Pathway Studies
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Glioma Diagnosis and Treatment
- Birth, Development, and Health
- Children's Physical and Motor Development
- Genetics and Physical Performance
University of Oregon
2015-2024
Eugene Research Institute
2014-2018
University of California, Davis
2015
University of North Carolina at Chapel Hill
2002
University of Toronto
1993
Marine Biological Laboratory
1985
Brandeis University
1981-1984
Utah State University
1980
ABSTRACT We have examined the development of specific muscle fiber types in zebrafish axial by labeling myogenic precursor cells with vital fluorescent dyes and following their subsequent differentiation fate. Two populations precursors, medial lateral, can be distinguished segmental plate position, morphology gene expression. The cells, known as adaxial are large, cuboidal adjacent to notochord that express myoD. Surprisingly, after somite formation, they migrate radially away from...
To facilitate molecular genetic analysis of vertebrate development, haploid genetics was used to construct a recombination map for the zebrafish Danio ( Brachydanio ) rerio . The consists 401 random amplified polymorphic DNAs (RAPDs) and 13 simple sequence repeats spaced at an average interval 5.8 centimorgans. Strategies that exploit advantages RAPD markers were developed quickly mapped lethal visible mutations placed cloned genes on map. This is useful position-based cloning mutant genes,...
Pigment patterns are useful for elucidating fundamental mechanisms of pattern formation and how these evolve. In zebrafish, several pigment cell classes interact to generate stripes, yet the developmental requirements origins cells remain poorly understood. Using zebrafish a related species, we identified roles thyroid hormone (TH) in development patterning, postembryonic more generally. We show that adult arise from distinct lineages having TH differential dependence can evolve within...
The gut microbiota is a complex consortium of microorganisms with the ability to influence important aspects host health and development. Harnessing this "microbial organ" for biomedical applications requires clarifying degree which bacterial factors act alone or in combination govern stability specific lineages. To address issue, we combined bacteriological manipulation light sheet fluorescence microscopy monitor dynamics defined two-species within vertebrate gut. We observed that interplay...
In zebrafish, individual primary motoneurons can be uniquely identified by their characteristic cell body positions and axonal projection patterns. The fate of remains plastic until just prior to axogenesis when they become committed particular identities. We find that distinct express combinations LIM homeobox genes. Expression precedes as well commitment, suggesting genes may contribute the specification motoneuronal fates. By transplanting them new spinal cord positions, we demonstrate...
Sonic hedgehog (Shh) signaling patterns many vertebrate tissues. shh mutations dramatically affect mouse ventral forebrain and floor plate but produce minor defects in zebrafish. Zebrafish have two mammalian Shh orthologs, sonic tiggy-winkle hedgehog, another gene, echidna that could overlapping functions. To examine the role of Hedgehog zebrafish, we characterized slow muscle omitted (smu) mutants. We show smu encodes a zebrafish ortholog Smoothened transduces signals. smoothened is...
To learn when cell fate differences first arise in the zebrafish trunk neural crest, individual premigratory crest cells were labeled intracellularly with fluorescent vital dyes, followed living embryos and complete lineages recorded. Although some of earliest to migrate produced derivatives multiple phenotypes, most appear be lineage-restricted, generating type-restricted precursors that produce single kinds derivatives. Further, phenotypes do so by precursors. Among various types...
Serial sampling of peripheral blood from six healthy adult male volunteers was performed during daytime waking and nighttime sleeping. In addition, sleep physiology assessed in all subjects (Ss) stages scored blind by standard criteria. Samples plasma were analyzed for cortisol (Co) levels, functional interleukin-1 (IL-1), interleukin-2 (IL-2) activity. Peripheral monocytes (PBM) assayed to evaluate natural killer (NK) activity mitogen responsiveness. Dramatic increase IL-1 along with...
Abstract We have exploited our ability to visualize and follow individual cells in situ, the living embryo, study development of trunk neural crest embryonic zebrafish. In most respects, zebrafish is similar other species: segregate from dorsal keel a rostrocaudal sequence, migrate ventrally along two pathways, give rise neurons peripheral nervous system, Schwann cells, pigment cells. However, some aspects differ those vertebrates: are significantly larger fewer number than avian embryos...
ABSTRACT Neural crest forms four major categories of derivatives: pigment cells, peripheral neurons, glia, and ectomesenchymal cells. Some early neural cells generate progeny several fates. How specific cell fates become specified is still poorly understood. Here we show that zebrafish embryos with mutations in the colourless gene have severe defects most crest-derived types, including neurons glia. In contrast, craniofacial skeleton medial fin mesenchyme are normal. These observations...
The segmental heritage of all vertebrates is evident in the character vertebral column. And yet, extent to which direct translation pattern from somitic mesoderm and de novo cell tissue interactions column remains a fundamental, unresolved issue. elements under debate include both anteroposterior regional specificity. Understanding how segmentation positional identity are patterned requires understanding cellular developmental biology. In this study, we characterized alignment somites...
In zebrafish, cells at the lateral edge of neural plate become Rohon-Beard primary sensory neurons or crest. Delta/Notch signaling is required for crest formation. ngn1 expressed in neurons; inhibiting Ngn1 activity prevents cell formation but not other neurons. Reducing embryos lacking restores formation, indicating inhibits neurogenesis without actively promoting also later development dorsal root ganglion however, and are necessarily derived from same precursor cell. We propose that...
1. The pyloric dilator (PD) and anterior burster (AB) neurons in the system of lobster stomatogastric ganglion are electrically coupled synchronously active. We have used lucifer yellow photoinactivation technique to separate connections made by PD motor from those AB interneuron. 2. Photoinactivation either two or single neuron allowed us compound inhibitory postsynaptic potentials (IPSPs) lateral (LP) (PY) resulting synchronous activity into AB-evoked PD-evoked components. These IPSPs...
The neurotransmitters mediating the synaptic interactions among neurons of pyloric system stomatogastric ganglion (STG) lobster, Panulirus interruptus, were examined using a combination electrophysiological, pharmacological, and biochemical techniques. Iontophoretically applied L-glutamate inhibited all motor system. This inhibitory response was blocked by low concentrations picrotoxin but unaffected atropine. anterior burster (AB) interneuron, dilator (PD) neurons, ventricular (VD) neuron...
Sustaining a balanced intestinal microbial community is critical for maintaining health and preventing chronic inflammation. The gut highly dynamic environment, subject to periodic waves of peristaltic activity. We hypothesized that this environment prerequisite the enteric nervous system (ENS), chief regulator physiological processes within gut, profoundly influences microbiota composition. found zebrafish lacking an ENS due mutation in Hirschsprung disease gene, sox10, develop...
ABSTRACT The vertebrate spinal cord consists of a large number different cell types in close proximity to one another. identities these cells appear be specified largely by information acquired from their local environments. We report here that cell-cell interactions, mediated zebrafish homologues the Drosophila melanogaster neurogenic gene, Delta, regulate specification diverse neuronal ventral cord. describe identification novel Delta gene expressed specifically nervous system and show,...