A5071508440- Vibrio bacteria research studies
- Gut microbiota and health
- Advanced Fluorescence Microscopy Techniques
- Bacterial biofilms and quorum sensing
- Cell Image Analysis Techniques
- Aquaculture disease management and microbiota
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Clostridium difficile and Clostridium perfringens research
- Photoacoustic and Ultrasonic Imaging
- Legionella and Acanthamoeba research
- Escherichia coli research studies
- Birth, Development, and Health
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Plant and Biological Electrophysiology Studies
- Microbial Community Ecology and Physiology
- Tryptophan and brain disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Molecular Biology Techniques and Applications
Princeton University
2016-2021
University of Oregon
2012-2016
Health First
2012-2016
The gut microbiota is a complex consortium of microorganisms with the ability to influence important aspects host health and development. Harnessing this "microbial organ" for biomedical applications requires clarifying degree which bacterial factors act alone or in combination govern stability specific lineages. To address issue, we combined bacteriological manipulation light sheet fluorescence microscopy monitor dynamics defined two-species within vertebrate gut. We observed that interplay...
ABSTRACT The vertebrate intestine is home to microbial ecosystems that play key roles in host development and health. Little known about the spatial temporal dynamics of these communities, limiting our understanding fundamental properties, such as their mechanisms growth, propagation, persistence. To address this, we inoculated initially germ-free zebrafish larvae with fluorescently labeled strains an Aeromonas species, representing abundant genus gut. Using light sheet fluorescence...
Abstract The combination of genetically encoded fluorescent proteins and three‐dimensional imaging enables cell‐type‐specific studies embryogenesis. Light sheet microscopy, in which fluorescence excitation is provided by a plane laser light, an appealing approach to live due its high speed efficient use photons. While the advantages rapid are apparent from recent work, importance low light levels development not well established. We examine zebrafish opercle, craniofacial bone that exhibits...
Bacteria communicate and collectively regulate gene expression using a process called quorum sensing (QS). QS relies on group-wide responses to signal molecules autoinducers. Here, we show that activates new program of multicellularity in Vibrio cholerae. This program, which term aggregation, is distinct from the canonical surface-biofilm formation represses. Aggregation induced by autoinducers, occurs rapidly cell suspensions, does not require division, features strikingly dissimilar those...
ABSTRACT The zebrafish, Danio rerio , is a powerful model for studying bacterial colonization of the vertebrate intestine, but genes required by commensal bacteria to colonize zebrafish gut have not yet been interrogated on genome-wide level. Here we apply high-throughput transposon mutagenesis screen Aeromonas veronii Hm21 and Vibrio sp. strain ZWU0020 during their intestine alone in competition with each other, as well different orders. We use these transposon-tagged libraries track...
Bacteria convert changes in sensory inputs into alterations gene expression, behavior, and lifestyles. A common lifestyle choice that bacteria make is whether to exhibit individual behavior exist the free-living planktonic state or engage collective form sessile communities called biofilms. Transitions between behaviors are controlled by chemical cell-to-cell communication process quorum sensing. Here, we show sensing represses Pseudomonas aeruginosa biofilm formation virulence activating...
Microbial colonization of the digestive tract is a crucial event in vertebrate development, required for maturation host immunity and establishment normal physiology. Advances genomic, proteomic, metabolomic technologies are providing more detailed picture constituents intestinal habitat, but these approaches lack spatial temporal resolution needed to characterize assembly dynamics microbial communities this complex environment. We report use light sheet microscopy provide high-resolution...
We describe a microscope capable of both light sheet fluorescence microscopy and differential interference contrast (DICM). The two imaging modes, which to the best our knowledge have not previously been combined, are complementary: provides three-dimensional fluorescently labelled components multicellular systems with high speed, large fields view, low phototoxicity, whereas reveals unlabelled neighbourhood tissues, organs, other structures inherent optical sectioning. Use single Nomarski...
Abstract The gut microbiota is a complex consortium of microorganisms with the ability to influence important aspects host health and development. Harnessing this ‘microbial organ’ for biomedical applications requires clarifying degree which bacterial factors act alone or in combination govern stability specific lineages. To address we combined bacteriological manipulation light sheet fluorescence microscopy monitor dynamics defined two-species within vertebrate gut. We observed that...
ABSTRACT Pseudomonas aeruginosa transitions between the free-swimming state and sessile biofilm mode during its pathogenic lifestyle. We show that quorum sensing represses P. formation virulence by activating expression of genes encoding KinB-AlgB two-component system. Phospho-AlgB genes, while KinB dephosphorylates, thereby, inactivates AlgB. discover photoreceptor BphP is kinase that, in response to light, phosphorylates activates Indeed, exposing light gene expression. To our knowledge,...
Bacteria can work as collectives to form multicellular communities. Vibrio cholerae , the bacterium that causes disease cholera in humans, forms aggregated communities liquid. Aggregate formation relies on a chemical communication process called quorum sensing.
<h3>Background</h3> Fulgent has developed a novel, polymer-based nano-encapsulating drug delivery platform providing unique attributes including completely amorphous formulations with improved solubility as well enhanced absorption, pharmacokinetic (PK) profiles, safety, and efficacy. This technology is broadly applicable to both IV oral – potentially shortening the development timeline. Importantly, this represents simple 'Plug Play' that can enable of multiple assets using same polymer...
Abstract Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on synthesis, release, and group-wide detection of small molecules autoinducers. In Vibrio cholerae , a multicellular community aggregation program occurs in liquid, during stationary phase, high-cell-density state. Here, we demonstrate that this consists two subprograms. one subprogram, which call void formation, structures form contain few cells but provide...