A5055322887- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Lymphatic System and Diseases
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Axon Guidance and Neuronal Signaling
- Retinoids in leukemia and cellular processes
- Genomics and Phylogenetic Studies
- Cytomegalovirus and herpesvirus research
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Barrier Structure and Function Studies
- Renal and related cancers
- Genetics, Aging, and Longevity in Model Organisms
Australian National University
2025
National University College
2025
New Generation University College
2025
University of Massachusetts Chan Medical School
2015-2024
UMass Memorial Health Care
2015
Heart and Stroke Foundation
2013
University of Toronto
2013
University Health Network
2013
Massachusetts Academy of Math and Science
2011
Erasmus MC
2010
Chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) or ChIP genome tiling array analysis (ChIP-chip) have become standard technologies for genome-wide identification of DNA-binding protein target sites. A number algorithms been developed in parallel that allow binding sites from ChIP-seq ChIP-chip datasets and subsequent visualization the University California Santa Cruz (UCSC) Genome Browser as custom annotation tracks. However, summarizing these tracks...
Recent evidence indicates that acquisition of artery or vein identity during vascular development is governed, in part, by genetic mechanisms. The artery-specific expression a number Notch signaling genes mouse and zebrafish suggests this pathway may play role arterial-venous cell fate determination development. We show loss embryos leads to molecular defects differentiation, including markers ectopic venous within the dorsal aorta. Conversely, we find activation repression fate. Finally,...
Dicer is a central enzyme in microRNA (miRNA) processing. We identified Dicer-independent miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In contrast to other miRNAs, miR-451 levels were refractory dicer loss of function but reduced MZago2 (maternal-zygotic) mutants. found pre-miR-451 processing requires Ago2 activity vivo. mutants showed delayed erythropoiesis could be rescued by wild-type or miR-451-duplex not catalytically dead Ago2. Changing the secondary...
We assembled a DNA clone containing the 11,161-nt sequence of prototype rhabdovirus, vesicular stomatitis virus (VSV), such that it could be transcribed by bacteriophage T7 RNA polymerase to yield full-length positive-strand complementary VSV genome. Expression this in cells also expressing nucleocapsid protein and two subunits resulted production with growth characteristics wild-type VSV. Recovery from was verified (i) presence genetic tags generating restriction sites derived genome, (ii)...
We have used time-lapse multiphoton microscopy of living Tg(fli1:EGFP)y1 zebrafish embryos to examine how a patterned, functional network angiogenic blood vessels is generated in the early vertebrate trunk. Angiogenic vascular sprouts emerge from longitudinal trunk axial (the dorsal aorta and posterior cardinal vein) two spatially temporally distinct steps. Dorsal aorta-derived form an initial primary segments, followed by emergence vein-derived secondary that interact interconnect...
Abstract The recent establishment of recombination‐based cloning systems has greatly facilitated the analysis gene function by allowing rapid and high‐efficiency generation plasmid constructs. However, use such an approach in zebrafish requires availability recombination‐compatible plasmids that are appropriate for functional studies embryos. In this work, we describe construction validation Gateway compatible vectors based on commonly used plasmids. We have generated pCS‐based allow both...
ATAC-seq (Assays for Transposase-Accessible Chromatin using sequencing) is a recently developed technique genome-wide analysis of chromatin accessibility. Compared to earlier methods assaying accessibility, faster and easier perform, does not require cross-linking, has higher signal noise ratio, can be performed on small cell numbers. However, ensure successful experiment, step-by-step quality assurance processes, including both wet lab control in silico assessment, are essential. While...
The zebrafish mutant violet beauregarde (vbg) can be identified at two days post-fertilization by an abnormal circulation pattern in which most blood cells flow through a limited number of dilated cranial vessels and fail to perfuse the trunk tail. This phenotype cannot explained caudal vessel abnormalities or defect patterning, but instead stems from increase endothelial cell specific vessels. We show that vbg encodes activin receptor-like kinase 1 (Acvrl1; also known as Alk1), TGFbeta type...
Recent evidence indicates a specific role for vascular endothelial growth factor (Vegfa) during artery development in both zebrafish and mouse embryos, whereas less is known about signals that govern vein formation. In zebrafish, loss of vegfa blocks segmental formation reduces artery-specific gene expression, veins are largely unaffected. Here, we describe mutation the vegf receptor-2 homolog, kdra , which eliminates its kinase activity leads to defects development. We further find Flt4,...
The limited generation of neurons during adulthood is controlled by a balance between quiescence and recruitment neural stem cells (NSCs). We use here the germinal zone zebrafish adult telencephalon to examine how frequency NSC divisions regulated. show, using several <i>in vivo</i> techniques, that progenitors transit back forth quiescent dividing state, according varying levels Notch activity: induction drives into quiescence, whereas blocking massively reinitiates division subsequent...
In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find y10 encodes phospholipase C gamma-1 ( plcg1 ), known effector receptor tyrosine kinase signaling. further show embryos fail respond exogenous Vegf. Our results indicate Plcg1 functions specifically downstream Vegf during embryonic development govern arterial system.
Fibroblast growth factor (Fgf) proteins are important regulators of pharyngeal arch development. Analyses Fgf8 function in chick and mouse Fgf3 zebrafish have demonstrated a role for Fgfs the differentiation survival postmigratory neural crest cells (NCC) that give rise to skeleton. Here we describe, zebrafish, an earlier, essential regulating segmentation endoderm into pouches. Using time-lapse microscopy, show pouches form by directed lateral migration discrete clusters endodermal cells....