A5044152086- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- melanin and skin pigmentation
- Fish Ecology and Management Studies
- Animal Behavior and Reproduction
- Retinal Development and Disorders
- Hippo pathway signaling and YAP/TAZ
- Fish biology, ecology, and behavior
- Animal Genetics and Reproduction
- Cell Adhesion Molecules Research
- Neurobiology and Insect Physiology Research
- MicroRNA in disease regulation
- Amphibian and Reptile Biology
- Ichthyology and Marine Biology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Physiological and biochemical adaptations
- Reproductive biology and impacts on aquatic species
- Molecular Biology Techniques and Applications
- Aquaculture disease management and microbiota
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Biochemical Analysis and Sensing Techniques
- Fish Biology and Ecology Studies
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
University of Virginia
2017-2025
University of Washington
2009-2023
University of California, Los Angeles
2023
National Human Genome Research Institute
2023
National Institutes of Health
2023
Max Planck Institute for Heart and Lung Research
2023
Rhode Island College
2021
Providence College
2021
Seattle University
2006-2015
California Institute for Regenerative Medicine
2014
Abstract The zebrafish is a premier model organism yet lacks system for assigning postembryonic fish to developmental stages. To provide such staging series, we describe changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median pelvic fins, pigment pattern, scale formation, larval fin fold, skeleton. We further identify milestones placing into discrete relate these size age show...
Pigment patterns are useful for elucidating fundamental mechanisms of pattern formation and how these evolve. In zebrafish, several pigment cell classes interact to generate stripes, yet the developmental requirements origins cells remain poorly understood. Using zebrafish a related species, we identified roles thyroid hormone (TH) in development patterning, postembryonic more generally. We show that adult arise from distinct lineages having TH differential dependence can evolve within...
ABSTRACT Developmental mechanisms underlying traits expressed in larval and adult vertebrates remain largely unknown. Pigment patterns of fishes provide an opportunity to identify genes cell behaviors required for postembryonic morphogenesis differentiation. In the zebrafish, Danio rerio, pigment reflect spatial arrangements three classes neural crest-derived cells: black melanocytes, yellow xanthophores silver iridophores. We show that D. rerio pattern mutant panther ablates embryos adults...
Abstract The relative roles of the Kit receptor in promoting migration and survival amniote melanocytes are unresolved. We show that, zebrafish, Danio rerio, pigment pattern mutation sparse corresponds to an orthologue c-kit. This finding allows us further elucidate morphogenetic for this c-kit-related gene melanocyte morphogenesis. Our analyses zebrafish development demonstrate that c-kit identified study is required both normal embryonic melanocytes. also find contrast mouse, we have not...
Ectothermic vertebrates exhibit a diverse array of adult pigment patterns. A common element these patterns is alternating dark and light stripes each comprising different classes neural crest-derived cells. In the zebrafish, Danio rerio, horizontal black melanophores yellow xanthophores are prominent feature pattern. fms mutant however, fail to develop melanophore severely disrupted. fmsencodes type III receptor tyrosine kinase expressed by their precursors closest known homologue kit, which...
Skin pigment patterns of vertebrates are a classic system for understanding fundamental mechanisms morphogenesis, differentiation, and pattern formation, recent studies zebrafish have started to elucidate the cellular interactions molecular underlying these processes. In this species, horizontal dark stripes melanophores alternate with light interstripes yellow or orange xanthophores iridescent iridophores. We showed previously that highly conserved zinc finger protein Basonuclin-2 (Bnc2) is...
The skin pigment pattern of zebrafish is a good model system in which to study the mechanism biological formation. Although it known that interactions between melanophores and xanthophores play key role formation adult stripes, molecular mechanisms for these remain largely unknown. Here, we show Delta/Notch signaling contributes interactions. Ablation yellow stripes induced death black suggesting require survival signal from distant xanthophores. We found deltaC notch1a were expressed by...
Thyroid hormone (TH) regulates diverse developmental events and can drive disparate cellular outcomes. In zebrafish, TH has opposite effects on neural crest derived pigment cells of the adult stripe pattern, limiting melanophore population expansion, yet increasing yellow/orange xanthophore numbers. To learn how elicits seemingly responses in having a common embryological origin, we analyzed individual transcriptomes from thousands crest-derived cells, reconstructed trajectories, identified...
The pigment cells of vertebrates serve a variety functions and generate stunning patterns. These are also implicated in human pathologies including melanoma. Whereas the events cell development have been studied extensively embryo, much less is known about morphogenesis differentiation these during post-embryonic stages. Previous studies zebrafish revealed genetically distinct populations embryonic adult melanophores, ectotherm homologue amniote melanocytes. Here, we use molecular markers,...
Changes in gene activity are essential for evolutionary diversification. Yet, elucidating the cellular behaviors that underlie modifications to adult form remains a profound challenge. We use neural crest-derived pigmentation of zebrafish and pearl danio uncover bases alternative pattern states. show stripes require novel class thin, fast projection promote Delta-Notch signaling over long distances from cells xanthophore lineage melanophores. Projections depended on microfilaments...
Macrophages have diverse functions in immunity as well development and homeostasis. We identified a function for these cells long-distance communication during postembryonic tissue remodeling. Ablation of macrophages zebrafish prevented melanophores from coalescing into adult pigment stripes. Melanophore organization depends on signals provided by the yellow xanthophore lineage via airinemes, long filamentous projections with vesicles at their tips. show that airineme extension originating...
The molecular genetic toolkit of the Mexican axolotl, a classic model organism, has matured to point where it is now possible identify genes for mutant phenotypes. We used positional cloning-candidate gene approach bases two historic axolotl pigment phenotypes: white and albino. White (d/d) mutants have defects in cell morphogenesis differentiation, whereas albino (a/a) lack melanin. identified transcriptional defect endothelin 3 (edn3), encoding peptide factor that promotes migration...