A5064111908- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Biosensors and Analytical Detection
- Advanced MRI Techniques and Applications
- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Neuroscience and Neural Engineering
- Fluid Dynamics and Turbulent Flows
- Lanthanide and Transition Metal Complexes
- Law and Political Science
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Public Administration and Political Analysis
- Advanced Chemical Sensor Technologies
- Molecular Communication and Nanonetworks
- Spectroscopy and Quantum Chemical Studies
- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Molecular Junctions and Nanostructures
- Microfluidic and Capillary Electrophoresis Applications
- Cystic Fibrosis Research Advances
- Microfluidic and Bio-sensing Technologies
- Advanced Sensor and Energy Harvesting Materials
Arizona State University
2020-2025
University of Oxford
2025
California State University, Dominguez Hills
2024
Northeastern University
2014-2023
Mayo Clinic
2023
Brighton and Sussex Medical School
2023
Universidad del Noreste
2014-2022
University of Toronto
1994-2020
Eindhoven University of Technology
2020
University of California, Los Angeles
2020
In vivo biosensors are emerging as powerful tools in biomedical research and diagnostic medicine. Distinct from "labels" or "imaging", designed for continuous long-term monitoring of target analytes real biological systems should be selective, sensitive, reversible biocompatible. Due to the challenges associated with meeting all analytical requirements, we found relatively few reports groups demonstrating devices that meet strict definition vivo. However, identified several case studies a...
Spherical optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced in sizes including 20 and 200 nm diameter. These sensors are fabricated a microemulsion consist of fluorescent indicators entrapped polyacrylamide matrix. A generalized polymerization method has developed that permits production containing any hydrophilic dye combination dyes the The PEBBLE matrix protects from interference proteins, allowing reliable vivo calibrations...
Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties pH-sensitive sensors three different calcium-selective are presented discussed. Each sensor combines an ion-selective fluorescent indicator ion-insensitive internal standard entrapped within acrylamide polymeric matrix. Calibrations linear ranges each sensor. The photobleaching dyes incorporated into is comparable to...
The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.
Personalized medicine could revolutionize how primary care physicians treat chronic disease and researchers study fundamental biological questions. To realize this goal, we need to develop more robust, modular tools imaging approaches for in vivo monitoring of analytes. In report, demonstrate that synthetic nanosensors can measure physiologic parameters with photoacoustic contrast, apply platform continuously track lithium levels vivo. Photoacoustic achieves depths are unattainable...
We have designed fluorescent nanosensors based on ion-selective optodes capable of detecting small molecules. By localizing the sensor components in a hydrophobic core, these are able to monitor dynamic changes concentration model analyte, glucose. The demonstrated this response vitro and also when injected subcutaneously into mice. tracked blood glucose levels vivo that were comparable measurements taken using glucometer. development offers an alternative, minimally invasive tool for...
We describe the synthesis and characterization of sodium-selective polymeric nanosensors that improves upon lifetime size previous fiberless nanosensors. Sonication is used to form polymer nanospheres contain all components needed for ion sensing. Even though small (approximately 120 nm), these sensors in solution on order a week. The surface coating has also been optimized stability, biocompatibility, ease chemical modification.
Ion-selective quantum dots (ISQDs) have been produced that are capable of measuring physiologically relevant ions (such as Na+, K+, Cl-, etc.). ISQDs consist dots, an ion-selective polymer matrix, and a biocompatible coating. work by selective ion extraction the matrix which leads to change in absorbance intensity embedded dye. This attenuates dot directly absorbing its fluorescence emission. As example this technology, sodium-selective ISQD measures sodium over range 1 mM M with 200-fold...
Regulation of sodium flux across the cell membrane plays a vital role in generation action potentials and regulation excitability cells such as cardiomyocytes neurons. Alteration channel function has been implicated diseases epilepsy, long QT syndrome, heart failure. However, single imaging dynamics limited due to narrow selection fluorescent indicators available researchers. Here we report, detection spatially defined activity during potentials. Fluorescent nanosensors that measure...
The tools for optically imaging cellular potassium concentrations in real-time are currently limited to a small set of molecular indicator dyes. Quantum dot-based nanosensors more photostable and tunable than organic indicators, but previous designs have fallen short size, sensitivity, selectivity. Here, we introduce small, sensitive, selective nanosensor measurements. A dynamic quencher modulates the fluorescence emitted by two different quantum dot species produce ratiometric signal. We...
Continuous in vivo monitoring of small molecule biomarkers requires biosensors with reversibility, sensitivity physiologically relevant ranges, and biological stability. Leveraging the real-time, label-free detection capability surface plasmon resonance (SPR) technology, a molecularly responsive hydrogel film is introduced to enhance sensitivity. This advanced biosensing platform utilizes split-aptamer-cross-linked hydrogels (aptagels) engineered using 8-arm poly(ethylene glycol) macromers,...
As bacteria grow and proliferate, they release a variety of volatile compounds that can be profiled used for speciation, providing an approach amenable to disease diagnosis through quick analysis clinical cultures as well patient breath analysis. practical alternative mass spectrometry detection whole cell pyrolysis approaches, we have developed methodology involves via sensitive, micromachined differential mobility spectrometer (microDMx), sampling headspace gases produced by growing in...