A5017406976- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nonlinear Optical Materials Studies
- Photoacoustic and Ultrasonic Imaging
- Nanofabrication and Lithography Techniques
- Cellular Mechanics and Interactions
- Spectroscopy and Quantum Chemical Studies
- Ovarian cancer diagnosis and treatment
- Advanced Chemical Physics Studies
- 3D Printing in Biomedical Research
- Optical Imaging and Spectroscopy Techniques
- Angiogenesis and VEGF in Cancer
- Optical Coherence Tomography Applications
- Molecular Biology Techniques and Applications
- Near-Field Optical Microscopy
- Photoreceptor and optogenetics research
- Glycosylation and Glycoproteins Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Protein Interaction Studies and Fluorescence Analysis
- Cancer Research and Treatments
- Photodynamic Therapy Research Studies
- Quantum, superfluid, helium dynamics
- Connective tissue disorders research
- Advanced Biosensing Techniques and Applications
- Ocular and Laser Science Research
University of Wisconsin–Madison
2015-2024
University of Wisconsin Carbone Cancer Center
2018-2024
University of Alabama at Birmingham
2017
Marymount University
2017
University of Minnesota
2017
Optica
2016
Bellingham Technical College
2015
University of Connecticut
2001-2012
UConn Health
2001-2010
In-Q-Tel
2009
Conventional 3-dimensional (3D) printing techniques cannot produce structures of the size at which individual cells interact.Here, we used multiphoton-excited 3D to generate a native-like extracellular matrix scaffold with submicron resolution and then seeded cardiomyocytes, smooth muscle cells, endothelial that had been differentiated from human-induced pluripotent stem cell-derived cardiac patch (hCMP), was subsequently evaluated in murine model myocardial infarction.The ≈50 000 (in 2:1:1...
Abstract Background Remodeling of the extracellular matrix (ECM) has been implicated in ovarian cancer, and we hypothesize that these alterations may provide a better optical marker early disease than currently available imaging/screening methods understanding their physical manifestations will insight into invasion. Methods For this investigation use Second Harmonic Generation (SHG) imaging microcopy to study changes structure ECM human normal malignant ex vivo biopsies. This method...
The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.
Abstract In this review, the authors discuss underlying principles of SHG and specific factors that affect generation properties describe essential components a instrument. addition, results on recent progress impact microscopy has made in different areas biology medicine are presented. particular, focus disease diagnosis basic research associated with connected tissues, musculo‐skeletal disorders, epithelial cancers. The presentation is concluded by offering perspective future technical...
Nonlinear multiphoton photo-cross-linking and photopolymerization of proteins polymers in solution have been used to direct the three-dimensional assembly micron scale objects. Two aspects fabricated proteinatious matrixes are examined this paper: efficiency protein application as sustained release devices. The photoactivated cross-linking bovine serum albumin fibrinogen, using rose bengal, determined found vary with photosensitizer concentration. This concentration dependence suggests that...
Second Harmonic Generation (SHG) microscopy has been previously used to describe the morphology of collagen in extracellular matrix (ECM) different stages invasion breast cancer. Here this concept is extended by using SHG provide quantitative discrimination self-assembled gels, consisting mixtures type I (Col I) and V V) isoforms which serve as models changes ECM during vivo. To investigate if sensitive due Col incorporation into fibrils, gels were prepared with 0-20% balance I. Using...
In this perspective, we discuss how the nonlinear optical technique of second-harmonic generation (SHG) microscopy has been used to greatly enhance our understanding tumor microenvironment (TME) breast and ovarian cancer. Striking changes in collagen architecture are associated with these epithelial cancers, SHG can image great sensitivity specificity submicrometer resolution. This information not historically exploited by pathologists but potential diagnostic prognostic capabilities. We...
Pump–probe techniques are used in conjunction with a tandem time-of-flight mass spectrometer to investigate the I...I− cage recombination dynamics following I−2 photodissociation size-selected I−2(CO2)n cluster ions. The absorption recovery, which reflects and vibrational relaxation of photodissociated I−2, exhibits strong size dependence range n=13–15. Over this limited range, recovery time decreases from ∼40 ps (n≤12) ∼10 (n≥15). In addition, recurrence is observed at ≊2 larger clusters...
Nuclear envelope breakdown was investigated during meiotic maturation of starfish oocytes. Fluorescent 70-kDa dextran entry, as monitored by confocal microscopy, consists two phases, a slow uniform increase and then massive wave. From quantitative analysis the first phase from imaging green fluorescent protein chimeras, we conclude that nuclear pore disassembly begins several minutes before breakdown. The best fit for second entry is with spreading disruption membrane permeability barrier...
Multiphoton excited (MPE) photochemistry is used to fabricate model tissue engineering scaffolds directly from types I, II, and IV collagen. A modified benzophenone dimer (BPD) provides the photoactivation becomes incorporated into resulting collagen matrixes. Unlike xanthene photochemistries, can be in acidic environments, where most forms of have greatest solubility. The minimum feature sizes are investigated by using two- three-photon excitation, latter for superior "resolution" suggests...
We have used quantitative second harmonic generation (SHG) imaging microscopy to investigate the collagen matrix organization in oim mouse model for human osteogenesis imperfecta (OI). OI is a heritable disease which type I fibrils are either abnormally organized or small, resulting clinical presentation of recurrent bone fractures and other pathologies related collagen-comprised tissues. Exploiting exquisite sensitivity SHG supramolecular assembly, we investigated whether this approach can...
Determining the health of muscle cells by in vivo imaging could impact diagnosis and monitoring a large number congenital acquired muscular or cardiac disorders. However, currently used technologies are hampered insufficient resolution, lack specificity, invasiveness. We have combined intrinsic optical second-harmonic generation from sarcomeric myosin with novel mathematical treatment striation pattern analysis, to obtain measures contractile integrity that correlate strongly neuromuscular...