A5082090157- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Congenital heart defects research
- Cancer Cells and Metastasis
- Cardiac Fibrosis and Remodeling
- Microfluidic and Bio-sensing Technologies
- Cardiomyopathy and Myosin Studies
- Cancer Genomics and Diagnostics
- Cell Image Analysis Techniques
- Additive Manufacturing and 3D Printing Technologies
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Pancreatic function and diabetes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Muscle Physiology and Disorders
- Biomedical and Engineering Education
- Cancer-related Molecular Pathways
University of Minnesota
2016-2025
Twin Cities Orthopedics
2015-2024
Colorado School of Mines
2024
University of Minnesota System
2023-2024
Minneapolis Heart Institute Foundation
2015-2023
University of Wisconsin–Madison
2009-2018
University of Alabama at Birmingham
2017
Material Sciences (United States)
2010-2015
Optica
2010-2015
In-Q-Tel
2011
Conventional 3-dimensional (3D) printing techniques cannot produce structures of the size at which individual cells interact.Here, we used multiphoton-excited 3D to generate a native-like extracellular matrix scaffold with submicron resolution and then seeded cardiomyocytes, smooth muscle cells, endothelial that had been differentiated from human-induced pluripotent stem cell-derived cardiac patch (hCMP), was subsequently evaluated in murine model myocardial infarction.The ≈50 000 (in 2:1:1...
Rationale: One goal of cardiac tissue engineering is the generation a living, human pump in vitro that could replace animal models and eventually serve as an vivo therapeutic. Models replicate geometrically complex structure heart, harboring chambers large vessels with soft biomaterials, can be achieved using 3-dimensional bioprinting. Yet, inclusion contiguous, living muscle to support function has not been achieved. This largely due challenge attaining high densities cardiomyocytes—a...
Although cancer cell fusion has been suggested as a mechanism of metastasis, the underlying mechanisms defining this process are poorly understood. In recent study, apoptotic cells were newly identified type cue that induces signaling via phosphatidylserine receptors to promote myoblasts. The microenvironment breast tumors is often hypoxic, and because apoptosis greatly increased in hypoxic conditions, we decided investigate whether with mesenchymal stem/multipotent stromal (MSCs) involves...
Abstract The design and development of novel methodologies customized materials to fabricate patient‐specific 3D printed organ models with integrated sensing capabilities can yield advances in smart surgical aids for preoperative planning rehearsal. Here, prostate are demonstrated physical properties tissue soft electronic sensors using custom‐formulated polymeric inks. show high quantitative fidelity static dynamic mechanical properties, optical characteristics, anatomical geometries...
The plasticity and immunomodulatory capacity of mesenchymal stem cells (MSCs) have spurred clinical use in recent years. However, outcomes vary many ascribe inconsistency to the tissue source MSCs. Yet unconsidered is extent heterogeneity individual MSCs from a given with respect differentiation potential immune regulatory function. Here we single-cell RNA-seq assess transcriptional diversity murine derived bone marrow. We found genes associated MSC multipotency were expressed at high level...
The extracellular matrix (ECM) of the embryonic heart guides assembly and maturation cardiac cell types and, thus, may serve as a useful template, or blueprint, for fabrication scaffolds tissue engineering. Surprisingly, characterization ECM with development is scattered fails to comprehensively reflect spatiotemporal dynamics making it difficult apply engineering efforts. objective this work was define blueprint organization, localization, relative amount four essential proteins, collagen I...
Journal Article Harnessing endogenous growth factor activity modulates stem cell behavior Get access Gregory A. Hudalla, Hudalla Department of Biomedical Engineering, University Wisconsin, 2130 Engineering Centers Building, 1550 Drive, Madison, WI 53706, USA. Fax: 608 265 9239; Tel: 262 2224 Search for other works by this author on: Oxford Academic Google Scholar Nicholas Kouris, Kouris Justin T. Koepsel, Koepsel Brenda M. Ogle, Ogle William L. Murphy 2224Department Pharmacology, Health...
Abstract The extracellular matrix (ECM) impacts stem cell differentiation, but identifying formulations supportive of differentiation is challenging in 3D models. Prior efforts involving combinatorial ECM arrays seemed intuitively advantageous. We propose an alternative that suggests reducing sample size and technological burden can be beneficial accessible when coupled to design experiments approaches. predict optimized could augment cardiomyocytes derived vitro . employed native chemical...
Over the past two decades Biomedical Engineering has emerged as a major discipline that bridges societal needs of human health care with development novel technologies. Every medical institution is now equipped at varying degrees sophistication ability to monitor in both non-invasive and invasive modes. The multiple scales which physiology can be interrogated provide profound perspective on disease. We are nexus creating "avatars" (herein defined an extension "digital twins")...
Background/Objectives: Focused ultrasound has advantages as an external stimulus for drug delivery it is non-invasive, high precision and can penetrate deep into tissues. Here, we report a gold-plated alginate (ALG) hydrogel system that retains highly water-soluble small-molecule fluorescein sharp off/on release after exposure. Methods: The ALG crosslinked beads with calcium chloride layered polycation to adjust the surface charge adsorption of catalytic platinum nanoparticles (Pt NPs)....
Extracellular matrix proteins (ECMs) guide differentiation of adult stem cells, but the temporal distribution (i.e., heterogeneity) in a given population has not been investigated. We tested effect individual ECM on lineage commitment human bone marrow–derived mesenchymal cells (MSCs) over time. exposed cell populations to representing primary tissue structures body collagens type I, III, IV; laminin; and fibronectin) determined at 1, 7, 14 days. found that can participate formation fibrils...
Evidence suggests that transplanted mesenchymal stem cells (MSCs) can aid recovery of damaged myocardium caused by myocardial infarction. One possible mechanism for MSC-mediated is reprogramming after cell fusion between MSCs and recipient cardiac cells. We used a Cre/LoxP-based luciferase reporter system coupled to biophotonic imaging detect human pluripotent cell-derived organs living mice. Human MSCs, with transient expression viral fusogen, were delivered the murine heart via collagen...
Limiting the precise study of biochemical impact whole molecule extracellular matrix (ECM) proteins on stem cell differentiation is lack 3D in vitro models that can accommodate many different types ECM. Here we sought to generate such a system while maintaining consistent mechanical properties and supporting survival. To this end, used native chemical ligation cross-link poly(ethylene glycol) macromonomers under mild conditions entrapping ECM (termed composites) cells. Sufficiently low...
To understand complex micro/nanoscale ECM stem cell interactions, reproducible in vitro models are needed that can strictly recapitulate the relative content and spatial arrangement of native tissue. Additionally, whole proteins required to most accurately reflect binding dynamics. address this need, we use multiphoton excited photochemistry create 3D protein constructs or "modules" study how governs migration. The were created from mixtures BSA/laminin (LN) BSA alone, whose comparison...