A5034479866- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- 3D Printing in Biomedical Research
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Diabetic Foot Ulcer Assessment and Management
- Extracellular vesicles in disease
- Signaling Pathways in Disease
- Diet and metabolism studies
- Protease and Inhibitor Mechanisms
- Cardiac Valve Diseases and Treatments
- Cardiovascular Function and Risk Factors
- Genetics and Neurodevelopmental Disorders
- Graphene and Nanomaterials Applications
- Manufacturing Process and Optimization
- Congenital Heart Disease Studies
- Thermal Regulation in Medicine
- Nanofabrication and Lithography Techniques
- Pharmacological Effects and Assays
- Cancer Cells and Metastasis
University of Wisconsin–Madison
2015-2025
University of Wisconsin System
2017
Abstract Cardiac fibroblasts (CFs) play critical roles in heart development, homeostasis, and disease. The limited availability of human CFs from native impedes investigations CF biology their role Human pluripotent stem cells (hPSCs) provide a highly renewable genetically defined cell source, but efficient methods to generate hPSCs have not been described. Here, we show differentiation using sequential modulation Wnt FGF signaling second field progenitors that efficiently give rise...
Abstract Cell tracking is a critical component of the safety and efficacy evaluation therapeutic cell products. To date, cell-tracking modalities have been hampered by poor resolution, low sensitivity, inability to track cells beyond shortterm. Three-dimensional (3D) cryo-imaging coregisters fluorescent bright-field microcopy images allows for single-cell quantification within 3D organ volume. We hypothesized that could be used measure biodistribution clearance after intravenous infusion in...
In the setting of radiation-induced trauma, exposure to high levels radiation can cause an acute syndrome (ARS) causing bone marrow (BM) failure, leading life-threatening infections, anemia, and thrombocytopenia. We have previously shown that human macrophages educated with mesenchymal stem cells (MSCs) by coculture significantly enhance survival mice exposed lethal irradiation. this study, we investigated whether exosomes isolated from MSCs could replace direct generate exosome (EEMs)....
Background Atrial fibrillation often occurs in the setting of hypertension and associated atrial dilation with pathologically increased cardiomyocyte stretch. In dilation, mechanoelectric feedback has been linked to development ectopic beats that trigger paroxysmal mainly originating from pulmonary veins ( PVs ). However, precise mechanisms remain poorly understood. Methods Results We identify mechanosensitive, swelling‐activated chloride ion channels I C l,swell ) as a crucial component...
Recent studies indicate that a substantial amount of metabolically active brown adipose tissue (BAT) exists in adult humans. Given the unique ability BAT to convert calories heat, there is intense interest understanding regulation metabolism hopes its manipulation might be an effective way expending excess calories. Because established role AMP-activated protein kinase (AMPK) as "metabolic master switch" and extremely high levels activity BAT, it was hypothesized AMPK play central regulating...
Abstract Aims Fibroblast to myofibroblast trans‐differentiation with altered bioenergetics precedes cardiac fibrosis (CF). Either prevention of differentiation or promotion de‐differentiation could mitigate CF‐related pathologies. We determined whether 3‐hydroxy‐3‐methyl‐glutaryl‐coenzyme A (HMG‐CoA) reductase inhibitors—statins, commonly prescribed patients at risk heart failure (HF)—can de‐differentiate myofibroblasts, alter cellular bioenergetics, and impact the human ventricular...
Right ventricular failure (RVF) is a common cause of death in patients suffering from pulmonary arterial hypertension (PAH). The current treatment for PAH only moderately improves symptoms, and RVF ultimately occurs. Therefore, it necessary to develop new strategies protect against right ventricle (RV) maladaptation despite progression. In this study, we hypothesize that local mesenchymal stem cell (MSC) delivery via novel bioscaffold can improve RV function persistent PAH. To test our...
Introduction: Fibroblasts are mesenchymal cells that predominantly produce and maintain the extracellular matrix (ECM) critical mediators of injury response. In heart, valve interstitial (VICs) a population fibroblasts responsible for maintaining structure function heart valves. These regionally distinct from myocardial fibroblasts, including left ventricular cardiac (LVCFBs), which located in myocardium close vicinity to cardiomyocytes. Here, we hypothesize these subpopulations...
Journal Article High-content adhesion assay to address limited cell samples Get access Jay W. Warrick, Warrick Biomedical Engineering, University of Wisconsin, Madison, WI, USA Search for other works by this author on: Oxford Academic Google Scholar Edmond K. Young, Young Eric G. Schmuck, Schmuck School Medicine and Public Health, Kurt Saupe, Saupe David J. Beebe E-mail: djbeebe@wisc.edu Integrative Biology, Volume 5, Issue 4, April 2013, Pages 720–727, https://doi.org/10.1039/c3ib20224k...