A5039128197- CAR-T cell therapy research
- Microbial Metabolic Engineering and Bioproduction
- Cancer Immunotherapy and Biomarkers
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Nanofabrication and Lithography Techniques
- Gene Regulatory Network Analysis
- Silicon Carbide Semiconductor Technologies
- Electrowetting and Microfluidic Technologies
- Molecular Communication and Nanonetworks
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
- Smart Grid and Power Systems
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Barrier Structure and Function Studies
- Microfluidic and Capillary Electrophoresis Applications
- Electrostatic Discharge in Electronics
- Nanowire Synthesis and Applications
- Cardiac Valve Diseases and Treatments
University of Wisconsin–Madison
2021-2025
Chinese Academy of Sciences
2011-2024
Shenyang Institute of Computing Technology (China)
2022-2024
University of Chinese Academy of Sciences
2011-2024
Institute of Biophysics
2011-2024
University of California, Los Angeles
2020-2022
University of California, Davis
2010-2018
Shenzhen Institutes of Advanced Technology
2018
University of Science and Technology of China
2018
University of California System
2017
Abstract Lipid droplets (LDs) are multi-functional organelles consisting of a neutral lipid core surrounded by phospholipid monolayer, and exist in organisms ranging from bacteria to humans. Here we study the functions LDs oleaginous bacterium Rhodococcus jostii . We show that these bind genomic DNA through major LD protein, MLDS, which increases survival rate bacterial cells under nutritional genotoxic stress. MLDS expression is regulated transcriptional regulator, MLDSR, binds operator...
Storage of cellular triacylglycerols (TAGs) in lipid droplets (LDs) has been linked to the progression many metabolic diseases humans, and development biofuels from plants microorganisms. However, biogenesis dynamics LDs are poorly understood. Compared with other organisms, bacteria seem be a better model system for studying LD biology, because they relatively simple highly efficient converting biomass TAG. We obtained purified Rhodococcus sp. RHA1, bacterium that can produce TAG carbon...
Rhodococcus opacus strain PD630 (R. PD630), is an oleaginous bacterium, and also one of few prokaryotic organisms that contain lipid droplets (LDs). LD important organelle for storage but intercellular communication regarding energy metabolism, yet a poorly understood cellular organelle. To understand the dynamics using simple model organism, we conducted series comprehensive omics studies R. including complete genome, transcriptome proteome analysis. The genome encodes 8947 genes are...
ABSTRACT Lipid droplets (LDs) are ubiquitous organelles that serve as a neutral lipid reservoir and hub for metabolism. Manipulating LD formation, evolution, mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels chemicals. However, key factors regulating dynamics remain poorly characterized. Here we purified LDs identified LD-associated proteins from cells lipid-producing yeast Rhodosporidium toruloides cultured under nutrient-rich, nitrogen-limited,...
The functioning of synthetic gene circuits depends on their local chemical context defined by the types and concentrations biomolecules in surrounding milieu that influences transcription translation. This chemical-context dependence arises from significant yet unknown cross talk between engineered components, host cells, environmental factors has been a persistent challenge for biology. Here, we show sensitivity networks to extracellular contexts can be minimized, designed functions...
Differentiating endothelial cells (ECs) from human pluripotent stem (hPSCs) typically takes 2 weeks and requires parameter optimization. Overexpression of cell type-specific transcription factors in hPSCs has shown efficient differentiation into various types. ETV2, a crucial factor for fate, can be overexpressed to induce rapid facile EC (iETV2-ECs). We developed two-stage strategy which involves differentiating inducible ETV2-overexpressing basal induction medium during stage I expanding...
Abstract Background Three common isoforms of the apolipoprotein E ( APOE ) gene - APOE2 , APOE3 and APOE4 hold varying significance in Alzheimer’s Disease (AD) risk. The allele is strongest known genetic risk factor for late-onset (AD), its expression has been shown to correlate with increased central nervous system (CNS) amyloid deposition accelerated neurodegeneration. Conversely, associated reduced AD lower CNS burden. Recent clinical data have suggested that blood-brain barrier (BBB)...
A multi-parametric gradient generation system has been established for multi-dimensional, high-throughput, and low-consumption quantitative analysis of a synthetic genetic module.
Manual micropipettes are the most heavily used liquid handling devices in biological and chemical laboratories; however, they suffer from low precision for volumes under 1 μl inevitable human errors. For a manual device, errors introduced pose potential risks of failed experiments, inaccurate results, financial costs. Meanwhile, can cause severe quantification high heterogeneity outcomes, becoming bottleneck reaction miniaturization quantitative research biochemical labs. Here, we report...
Introduction: Fibroblasts are mesenchymal cells that predominantly produce and maintain the extracellular matrix (ECM) critical mediators of injury response. In heart, valve interstitial (VICs) a population fibroblasts responsible for maintaining structure function heart valves. These regionally distinct from myocardial fibroblasts, including left ventricular cardiac (LVCFBs), which located in myocardium close vicinity to cardiomyocytes. Here, we hypothesize these subpopulations...
SUMMARY Chimeric antigen receptors (CARs) are modular proteins capable of redirecting immune cells toward a wide variety disease-associated antigens. Here, we explore the effects CAR protein sequence and structure on CAR-T cell function. Based empirical observation that CD20 CARs with similar sequences exhibit divergent tonic-signaling anti-tumor activities, devised engineering strategies aimed to improve function by tuning intensity tonic signaling. We found designed low but non-zero levels...
Systematic determination of gene function is an essential step in fully understanding the precise contribution each for proper execution molecular functions cell. Gene functional linkage defined as to describe relationship a group genes with similar functions. With thousands genomes sequenced, there arises great opportunity utilize evolutionary information identify linkages. To this end, we established computational method (called TRACE) trace footprints through network constructed from 341...
Both the energy shortage and pollution tend to slow down economic development affect our daily lives. Some microorganisms not only can digest pollutants, but also convert pollutant metabolites triacylglycerol (TAG) that be used produce biodiesel. Here, we present results showing bacterium strain LDS5, a mutant of Rhodococcus sp. RHA1 (RHA1) generated in lab, could grow well chemithermomechanical pulping (CTMP) effluent, type paper mill wastewater, reduce chemical oxygen demand (CODCr)...
Lipid droplets (LD) are highly dynamic organelles specialized for the regulation of energy storage and cellular homeostasis. LD consist a neutral lipid core surrounded by phospholipid monolayer membrane with embedded proteins, most which involved in In this study, we focused on one major sterol C24-methyltransferase, encoded ERG6. We found that absence Erg6p resulted an increased accumulation yeast perilipin Pet10p LD, while disruption PET10 was accompanied over-accumulation. An observed...
This paper reports a microfluidic enabled print-to-screen (P2S) platform, which can achieve low-cost, high-throughput, high-precision printing with plug-and-play disposable cartridge, as well parallel screening minimized crosstalk. The performance of the P2S platform is characterized and, using this cell-killing 3-out-of-10 drug combinations towards ovarian cancer cell is, for first time, studied and result, 15 out 175 are newly identified to exert potent toxicity. demonstrates applicability...