Phosphatidylinositol transfer proteins (PITPs) are versatile required for signal transduction and membrane traffic. The best characterized mammalian PITPs the Class I PITPs, PITPα (PITPNA) PITPβ (PITPNB), which single domain with a hydrophobic cavity that binds phosphatidylinositol (PI) or phosphatidylcholine molecule. In this study, we report lipid binding properties of an uncharacterized soluble PITP, protein, cytoplasmic 1 (PITPNC1) (alternative name, RdgBβ), II family. We show protein...

Glycerophosphocholine is formed via the deacylation of phospholipid phosphatidylcholine. The protein encoded by Saccharomyces cerevisiae open reading frame YPL110c effects glycerophosphocholine metabolism in vivo, most likely acting as a phosphodiesterase. Deletion causes an accumulation cells prelabeled with [14C]choline. Correspondingly, overexpression results reduced intracellular Glycerophospho[3H]choline supplied growth medium accumulates to much greater extent fraction YPL110Delta...

Vesicles formed by the COPI complex function in retrograde transport from Golgi to endoplasmic reticulum (ER). Phosphatidylinositol transfer protein beta (PITPbeta), an essential that possesses phosphatidylinositol (PtdIns) and phosphatidylcholine (PtdCho) lipid activity is known localise ER but its role these membrane systems not clear. To examine of PITPbeta at Golgi-ER interface, RNA interference (RNAi) was used knockdown expression HeLa cells. Depletion leads a decrease PtdIns(4)P...

Seed oil from flax (Linum usitatissimum) is enriched in α-linolenic acid (ALA; 18:3Δ9cis,12cis,15cis), but the biochemical processes underlying enrichment of seed with this polyunsaturated fatty are not fully elucidated. Here, a potential process involving catalytic actions long-chain acyl-CoA synthetase (LACS) and diacylglycerol acyltransferase (DGAT) proposed for ALA triacylglycerol (TAG). LACS catalyzes ATP-dependent activation free to form acyl-CoA, which turn may serve as an acyl-donor...

Punicic acid (PuA) is a polyunsaturated fatty with significant medical, biological, and nutraceutical properties. The primary source of punicic the pomegranate seed oil obtained from fruits trees that are mainly cultivated in subtropical tropical climates. To establish sustainable production PuA, various recombinant microorganisms plants have been explored as platforms limited efficiencies. In this study, oleaginous yeast Yarrowia lipolytica was employed host for PuA production. First,...

The product of the open reading frame YPL206c, Pgc1p, yeast Saccharomyces cerevisiae displays homology to bacterial and mammalian glycerophosphodiester phosphodiesterases. Deletion PGC1 causes an accumulation anionic phospholipid, phosphatidylglycerol (PG), especially under conditions inositol limitation. This PG was not caused by increased production phosphatidyl-glycerol phosphate or decreased consumption in formation cardiolipin, end pathway. pgc1Delta strain rather inactivation...

CKS proteins are evolutionarily conserved cyclin-dependent kinase (CDK) subunits whose functions incompletely understood. Mammals have two proteins. CKS1 acts as a cofactor to the ubiquitin ligase complex SCF(SKP2) promote degradation of CDK inhibitors, such p27. Little is known about role closely related CKS2. Using Cks2(-/-) knockout mouse model, we show that CKS2 counteracts and stabilizes Unopposed activity in cells leads loss The resulting unrestricted cyclin A/CDK2 accompanied by...

Sec14p of the yeast Saccharomyces cerevisiae is involved in protein secretion and regulation lipid synthesis turnover vivo , but acts as a phosphatidylinositol–phosphatidylcholine transfer vitro . In this work, five homologues Sec14p, Sfh1p–Sfh5p, were subjected to biochemical cell biological analysis get better view their physiological role. We show that overexpression SFH2 SFH4 suppressed sec14 growth defect more SFH1 less efficient way, whereas SFH3 SFH5 did not complement Using...

Of many lipid transfer proteins identified, all have been implicated in essential cellular processes, but the activity of none has demonstrated intact cells. Among these, phosphatidylinositol (PITP) are particular interest as they can bind to and (PtdIns)--the precursor important signalling molecules, phosphoinositides--and because functions neuronal development (PITPalpha) cytokinesis (PITPbeta). Structural analysis indicates that, cytosol, PITPs a 'closed' conformation completely shielding...

Calendic acid (CA) is a conjugated fatty with anti-cancer properties that widely present in seed oil of Calendula officinalis. Using the co-expression C. officinalis conjugases (CoFADX-1 or CoFADX-2) and Punica granatum desaturase (PgFAD2), we metabolically engineered synthesis CA yeast Schizosaccharomyces pombe without need for linoleic (LA) supplementation. The highest titer achieved accumulation were 4.4 mg/L 3.7 mg/g DCW PgFAD2 + CoFADX-2 recombinant strain cultivated at 16 °C 72 h,...

Abstract The role of cis ‐vaccenic acid (18:1n‐7) in the reduction unsaturated fatty acids toxicity was investigated baker's yeast Saccharomyces cerevisiae. quadruple mutant (QM, dga1Δ lro1Δ are1Δ are2Δ ) deficient enzymes responsible for triacylglycerol and steryl ester synthesis has been previously shown to be highly sensitive exogenous acids. We have found that accumulated during cultivation QM cells but not corresponding wild type strain. This accumulation accompanied by a palmitoleic...

Mitosis is a crucial stage in the cell cycle, controlled by vast network of regulators responding to multiple internal and external factors. The fission yeast Schizosaccharomyces pombe demonstrates catastrophic mitotic phenotypes due mutations or drug treatments. One factors provoking mitosis disturbed lipid metabolism, resulting from, for example, acetyl-CoA/biotin carboxylase (cut6), fatty acid synthase (fas2, also known as lsd1) transcriptional regulator metabolism (cbf11) genes, well...

Pdr16p belongs to the family of phosphatidylinositol transfer proteins in yeast. The absence results enhanced susceptibility azole antifungals Saccharomyces cerevisiae. In major fungal human pathogen Candida albicans, CaPDR16 is a contributing factor clinical resistance. current study was aimed at better understanding function Pdr16p, especially relation resistance S. We show that deletion PDR16 gene increased cerevisiae are used medicine and agriculture. Significant differences inhibition...

Pdr16p is considered a factor of clinical azole resistance in fungal pathogens. The most distinct phenotype yeast cells lacking their increased susceptibility to and morpholine antifungals. (also known as Sfh3p) Saccharomyces cerevisiae belongs the Sec14 family phosphatidylinositol transfer proteins. It facilitates (PI) between membrane compartments vitro systems. We generated Pdr16pE235A, K267A mutant defective PI binding. This binding deficient not able fulfill role protection against...

Yeast Sec14‐like phosphatidylinositol transfer proteins (PITPs) contain a hydrophobic cavity capable of accepting single molecule (PI) or another in mutually exclusive manner. We report here that two yeast Sec14 family PITPs, Pdr16p (Sfh3p) and Pdr17p (Sfh4p), possess high‐affinity binding towards lanosterol. To our knowledge, this is the first identification lanosterol proteins. In addition, pdr16Δpdr17Δ double mutant had significantly increased level cellular compared with corresponding...

Long-chain acyl-CoA synthetase (LACS, EC 6.2.1.3) catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which, in turn, serves as major acyl donor for various lipid metabolic pathways. Increasing size pool by enhancing LACS activity appears be a useful approach improve production and modify composition acid-derived compounds, such triacylglycerol. In present study, we aimed enzyme Arabidopsis thaliana LACS9 (AtLACS9) introducing random mutations into its cDNA using...

Punicic acid (PuA) is a high-value edible conjugated fatty with strong bioactivities and has important potential applications in nutraceutical, pharmaceutical, feeding, oleochemical industries. Since the production of PuA severely limited by fact that its natural source (pomegranate seed oil) not readily available on large scale, there considerable interest understanding biosynthesis accumulation this plant-based unusual transgenic microorganisms to support rational design biotechnological...

Punicic acid (PuA) is a conjugated fatty with wide range of nutraceutical properties naturally present in pomegranate seed oil. To meet the rising demand for oil, single-cell oil enriched PuA provides sustainable biomass-derived alternative. This study describes production PuA-enriched through engineering red yeast