A5082357799- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Antifungal resistance and susceptibility
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- Trypanosoma species research and implications
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Fermentation and Sensory Analysis
- Polyamine Metabolism and Applications
- Genomics, phytochemicals, and oxidative stress
- Light effects on plants
- Cancer, Hypoxia, and Metabolism
- Alcoholism and Thiamine Deficiency
- Ion channel regulation and function
- Cancer-related Molecular Pathways
- Pesticide Exposure and Toxicity
Slovak Academy of Sciences
2013-2024
Institute of Animal Biochemistry and Genetics of the Slovak Academy of Sciences
2012-2024
Oregon Health & Science University
2002
Carnegie Mellon University
1996
The <i>SEC14</i> gene encodes a phosphatidylinositol/phosphatidylcholine transfer protein essential for secretion and growth in yeast (1). Mutations (<i>cki1, cct1</i>, <i>cpt1</i>) the CDP-choline pathway phosphatidylcholine synthesis suppress <i>sec14</i> defect (2), permitting <i>sec14</i><sup>ts</sup> <i>cki1, sec14</i><sup>ts</sup> <i>cct1</i>, and<i>sec14</i><sup>ts</sup> <i>cpt1</i> strains to grow at the<i>sec14</i><sup>ts</sup> restrictive temperature. Previously, we reported that...
In yeast, mutations in the CDP-choline pathway for phosphatidylcholine biosynthesis permit cell to grow even when <i>SEC14</i> gene is completely deleted (Cleves, A., McGee, T., Whitters, E., Champion, K., Aitken, J., Dowhan, W., Goebl, M., and Bankaitis, V. (1991) <i>Cell</i> 64, 789–800). We report that strains carrying pathway, such as<i>cki1</i>, exhibit a choline excretion phenotype due production of during normal turnover phosphatidylcholine. Cells <i>cki1</i> combination...
Squalene is a valuable natural substance with several biotechnological applications. In the yeast Saccharomyces cerevisiae, it produced in isoprenoid pathway as first precursor dedicated to ergosterol biosynthesis. The aim of this study was explore potential squalene epoxidase encoded by ERG1 gene target for manipulating levels yeast. Highest (over 1000 μg per 109 cells) were induced specific point mutations that reduced activity and caused hypersensitivity terbinafine. This accumulation...
Significance During translation, ribosomes decode mRNAs in a sequential fashion. In this paper, we report the discovery of more than 80 translational bypassing elements (byps) 27–55 nt long mitochondrial protein-coding regions yeast Magnusiomyces capitatus. We demonstrate experimentally that byps are retained mRNA but not translated into protein. Byps somewhat resemble single bypass element bacteriophage T4 also display unique features. further discovered byp-like sequences other species,...
In yeast, as in other eukaryotes, phosphatidylcholine (PC) can be synthesized via methylation of phosphatidylethanolamine or from free choline the CDP-choline pathway. PC biosynthesis is required for repression phospholipid biosynthetic genes, including INO1 gene, response to inositol. this study, we analyzed effect mutations genes encoding enzymes involved on transcriptional regulation genes. We report that transcription inositol clearly dependent ongoing biosynthesis, but it independent...
Glycerophosphocholine is formed via the deacylation of phospholipid phosphatidylcholine. The protein encoded by Saccharomyces cerevisiae open reading frame YPL110c effects glycerophosphocholine metabolism in vivo, most likely acting as a phosphodiesterase. Deletion causes an accumulation cells prelabeled with [14C]choline. Correspondingly, overexpression results reduced intracellular Glycerophospho[3H]choline supplied growth medium accumulates to much greater extent fraction YPL110Delta...
The product of the open reading frame YPL206c, Pgc1p, yeast Saccharomyces cerevisiae displays homology to bacterial and mammalian glycerophosphodiester phosphodiesterases. Deletion PGC1 causes an accumulation anionic phospholipid, phosphatidylglycerol (PG), especially under conditions inositol limitation. This PG was not caused by increased production phosphatidyl-glycerol phosphate or decreased consumption in formation cardiolipin, end pathway. pgc1Delta strain rather inactivation...
Sec14p of the yeast Saccharomyces cerevisiae is involved in protein secretion and regulation lipid synthesis turnover vivo , but acts as a phosphatidylinositol–phosphatidylcholine transfer vitro . In this work, five homologues Sec14p, Sfh1p–Sfh5p, were subjected to biochemical cell biological analysis get better view their physiological role. We show that overexpression SFH2 SFH4 suppressed sec14 growth defect more SFH1 less efficient way, whereas SFH3 SFH5 did not complement Using...
Degradation of Saccharomyces cerevisiae G 1 cyclins Cln1 and Cln2 is mediated by the ubiquitin-proteasome pathway involves SCF E3 ubiquitin-ligase complex containing F-box protein Grr1 (SCF ).Here we identify domain that confers instability describe signals in result binding to rapid degradation.We demonstrate mutants lack a cluster four Cdc28 consensus phosphorylation sites are highly stabilized fail interact with vivo.Since one lies within PEST motif, sequence rich proline, aspartate or...
Abstract The role of cis ‐vaccenic acid (18:1n‐7) in the reduction unsaturated fatty acids toxicity was investigated baker's yeast Saccharomyces cerevisiae. quadruple mutant (QM, dga1Δ lro1Δ are1Δ are2Δ ) deficient enzymes responsible for triacylglycerol and steryl ester synthesis has been previously shown to be highly sensitive exogenous acids. We have found that accumulated during cultivation QM cells but not corresponding wild type strain. This accumulation accompanied by a palmitoleic...
Pdr16p belongs to the family of phosphatidylinositol transfer proteins in yeast. The absence results enhanced susceptibility azole antifungals Saccharomyces cerevisiae. In major fungal human pathogen Candida albicans, CaPDR16 is a contributing factor clinical resistance. current study was aimed at better understanding function Pdr16p, especially relation resistance S. We show that deletion PDR16 gene increased cerevisiae are used medicine and agriculture. Significant differences inhibition...
Pdr16p is considered a factor of clinical azole resistance in fungal pathogens. The most distinct phenotype yeast cells lacking their increased susceptibility to and morpholine antifungals. (also known as Sfh3p) Saccharomyces cerevisiae belongs the Sec14 family phosphatidylinositol transfer proteins. It facilitates (PI) between membrane compartments vitro systems. We generated Pdr16pE235A, K267A mutant defective PI binding. This binding deficient not able fulfill role protection against...
Selenium (Se) is a trace element that essential for human health as it takes part in many cellular processes. The response to this compound elicits very diverse processes including DNA damage and repair. Because an inorganic form of Se, sodium selenite (SeL), has often been numerous studies because Se used dietary supplement by the public, here, we elucidated mechanisms SeL-induced toxicity yeast Saccharomyces cerevisiae using combination systematic genetic transcriptome analysis. First,...
Yeast Sec14‐like phosphatidylinositol transfer proteins (PITPs) contain a hydrophobic cavity capable of accepting single molecule (PI) or another in mutually exclusive manner. We report here that two yeast Sec14 family PITPs, Pdr16p (Sfh3p) and Pdr17p (Sfh4p), possess high‐affinity binding towards lanosterol. To our knowledge, this is the first identification lanosterol proteins. In addition, pdr16Δpdr17Δ double mutant had significantly increased level cellular compared with corresponding...
In the yeast Saccharomyces cerevisiae, products of two genes (PSD1 and PSD2) are able to catalyze decarboxylation phosphatidylserine (PS) produce phosphatidylethanolamine (PE) (C. J. Clancey, S. Chang, W. Dowhan, Biol. Chem. 268:24580-24590, 1993; P. Trotter, Pedretti, D. R. Voelker, 268:21416-21424, P.J. 270:6062-6070, 1995). I report that major mitochondrial PS decarboxylase gene (PSD1) is transcriptionally regulated by inositol in a manner similar reported for other coregulated...
The fight against multidrug-resistant pathogens requires an understanding of the underlying cellular mechanisms. In this work, we isolate and characterize one multidrug resistance determinants in Kluyveromyces lactis, KlPDR16 gene. We show that KlPdr16p (345 aa), which belongs to KlPdr1p regulon, is a functional homologue Saccharomyces cerevisiae Pdr16p. Deletion resulted hypersensitivity K. lactis cells antifungal azoles, oligomycin, rhodamine 6G, 4-nitroquinoline-N-oxide alkali metal...
The INO1 gene of yeast is expressed in logarithmically growing, wild-type cells when inositol absent from the medium. However, repressed present during logarithmic growth and it also as enter stationary phase whether or not. In this report, we demonstrate that transient nitrogen limitation causes repression. repression response to shares many features common with presence inositol. Specifically, dependent upon a functional OPI1 product, requires ongoing phosphatidylcholine biosynthesis...