A5023003882- Microbial bioremediation and biosurfactants
- Plant tissue culture and regeneration
- Transplantation: Methods and Outcomes
- Toxic Organic Pollutants Impact
- Renal Transplantation Outcomes and Treatments
- Microbial Community Ecology and Physiology
- Pesticide and Herbicide Environmental Studies
- Enzyme-mediated dye degradation
- Transgenic Plants and Applications
- Enzyme Catalysis and Immobilization
- Antimicrobial Peptides and Activities
- Circadian rhythm and melatonin
- Plant Stress Responses and Tolerance
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Plant Genetic and Mutation Studies
- Microbial Metabolic Engineering and Bioproduction
- Organ Transplantation Techniques and Outcomes
- Chromium effects and bioremediation
- Heavy metals in environment
- Microplastics and Plastic Pollution
- Genomics and Phylogenetic Studies
- Genetically Modified Organisms Research
- Allelopathy and phytotoxic interactions
- Viral Infections and Immunology Research
The Metabolomics Innovation Centre
2018-2025
University of Alberta
2001-2025
Genomics (United Kingdom)
2024
Women and Children’s Health Research Institute
2019
University of Chemistry and Technology, Prague
2004-2013
Institute of Chemical Technology
1997-2013
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
1998-2012
Institut Pro Studium Literatury
2010
Czech Academy of Sciences
2010
Institute of Animal Biochemistry and Genetics of the Slovak Academy of Sciences
1996-2002
ABSTRACT The abundance, identities, and degradation abilities of indigenous polychlorinated biphenyl (PCB)-degrading bacteria associated with five species mature trees growing naturally in a contaminated site were investigated to identify plants that enhance the microbial PCB potential soil. Culturable degraders every plant examined both rhizosphere root zone, which was defined as bulk soil rooted. Significantly higher numbers (2.7- 56.7-fold-higher means) detected zones Austrian pine (...
Bacteria were identified associated with biodegradation of aromatic pollutants biphenyl, benzoate, and naphthalene in a long-term polychlorinated biphenyl- polyaromatic hydrocarbon-contaminated soil. In order to avoid biases culture-based approaches, stable isotope probing was applied combination sequence analysis 16 S rRNA gene pyrotags amplified from 13C-enriched DNA fractions. Special attention paid pyrosequencing data eliminate the errors caused by either generation amplicons (random...
We previously reported a system for assessing rejection in kidney transplant biopsies using microarray-based gene expression data, the Molecular Microscope® Diagnostic System (MMDx). The present study was designed to optimize accuracy and stability of MMDx diagnoses by replacing single machine learning classifiers with ensembles diverse classifier methods. also examined use automated report sign-outs agreement between multiple human interpreters molecular results. Ensembles generated that...
ABSTRACT Rhizoremediation of organic chemicals requires high-level expression biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain was shown to be an colonizer numerous plant rhizospheres, including alfalfa. Although derivative expressing polychlorinated biphenyl (PCB) (F113pcb) has been reported previously, this shows low level bph gene expression, limiting its rhizoremediation potential. Here, system...
ABSTRACT Bacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated (PCB)-contaminated soil vegetated by horseradish. Isolates identified using matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS). The usage MALDI Biotyper for the classification isolates was evaluated compared 16S rRNA gene sequence analysis. A wide spectrum bacteria isolated, with Arthrobacter , Serratia Rhodococcus Rhizobium...
Plasma donor-derived cell-free DNA (dd-cfDNA) is used to screen for rejection in heart transplants. We launched the Trifecta-Heart study ( ClinicalTrials.gov No. NCT04707872), an investigator-initiated, prospective trial, examine correlations between genome-wide molecular changes endomyocardial biopsies (EMBs) and plasma dd-cfDNA. The present report analyzes correlation of dd-cfDNA with gene expression EMBs from 4 vanguard centers compared these those 604 kidney transplant Trifecta-Kidney...
DNA-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used order to identify members of the microbial community that metabolize biphenyl rhizosphere horseradish (Armoracia rusticana) cultivated soil contaminated polychlorinated biphenyls (PCBs) compared initial, uncultivated bulk soil. On basis early and recurrent detection their 16S rRNA genes clone libraries constructed from [(13)C]DNA, Hydrogenophaga spp. appeared dominate catabolism...
In kidney transplant biopsies, inflammation in areas of atrophy-fibrosis (i-IFTA) is associated with increased risk failure, presumably because evoked by recent parenchymal injury from rejection or other insults, but some cases also have rejection. The present study explored the frequency i-IFTA, using histology Banff 2015 and a microarray-based molecular diagnostic system (MMDx). unselected indication biopsies (108 73 uninflamed IFTA [i0-IFTA], 53 no IFTA), i-IFTA occurred later, showed...
Especially tailored transgenic plants were prepared for the purpose of phytoremediation. In case heavy metals, high accumulation in is main requirement, particularly harvestable parts. Improvement a plant's ability to tolerate metals via introduction metallothionein (MT) genes has already been demonstrated by different authors. this study, an additional metal binding domain implemented protein was introduced order further enhance capacity. For reason, gene coding peptide containing six...