John S. Fletcher

ORCID: 0000-0002-9418-8571
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About
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Research Areas
  • Ion-surface interactions and analysis
  • Mass Spectrometry Techniques and Applications
  • Integrated Circuits and Semiconductor Failure Analysis
  • Pesticide and Herbicide Environmental Studies
  • Plant tissue culture and regeneration
  • Diamond and Carbon-based Materials Research
  • Bacterial Identification and Susceptibility Testing
  • Analytical chemistry methods development
  • Plant nutrient uptake and metabolism
  • Photosynthetic Processes and Mechanisms
  • Metabolomics and Mass Spectrometry Studies
  • Allelopathy and phytotoxic interactions
  • Spectroscopy Techniques in Biomedical and Chemical Research
  • Nanopore and Nanochannel Transport Studies
  • Weed Control and Herbicide Applications
  • Nuclear Physics and Applications
  • Flowering Plant Growth and Cultivation
  • Plant Stress Responses and Tolerance
  • Pesticide Residue Analysis and Safety
  • Plant Pathogens and Fungal Diseases
  • Plant Molecular Biology Research
  • Fungal Biology and Applications
  • Microbial bioremediation and biosurfactants
  • Microbial Metabolic Engineering and Bioproduction
  • Plant Growth Enhancement Techniques

University of Gothenburg
2015-2025

Chalmers University of Technology
2010-2020

University of Manchester
2004-2014

Materials Technology (United Kingdom)
2010

Pennsylvania State University
2010

University of Oklahoma
1993-2009

Ionoptika (United Kingdom)
2008

University of York
2003

Colorado State University
2002

Science Oxford
2000

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) using buckminsterfullerene (C60) as the primary source has ability to generate chemical images of surfaces with high sensitivities and minimal damage. We studied application C60+ depth profile a biological cell surface in controlled manner subsequently image revealed subsurfaces, order three-dimensional molecular system. Such an analytical tool not only enables localization species be mapped but also biomolecular distribution function...

10.1021/ac061370u article EN Analytical Chemistry 2007-02-16

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) has unique capabilities in the area of high-resolution spectrometric imaging biological samples. The technique offers parallel detection native and non-native molecules at physiological concentrations with potentially submicrometer spatial resolution. Recent advances SIMS technology have been focused on generating new sources that can turn be used to eject more intact molecular characteristic species from a sample. introduction...

10.1021/ac8015278 article EN Analytical Chemistry 2008-10-25

ABSTRACT The abundance, identities, and degradation abilities of indigenous polychlorinated biphenyl (PCB)-degrading bacteria associated with five species mature trees growing naturally in a contaminated site were investigated to identify plants that enhance the microbial PCB potential soil. Culturable degraders every plant examined both rhizosphere root zone, which was defined as bulk soil rooted. Significantly higher numbers (2.7- 56.7-fold-higher means) detected zones Austrian pine (...

10.1128/aem.72.4.2331-2342.2006 article EN Applied and Environmental Microbiology 2006-04-01

The growth dynamics and phenolic content of mulberry (Morus sp.) fine roots (<1 mm diameter) were determined examined in relationship to rhizosphere remediation recalcitrant soil contaminants. Root turnover measurements rhizotron-grown plants showed that 58% the produced during a 6-month growing season (June−November) died at end season. concentration compounds increased approximately 2-fold later stages season, total dead reached maximum value 38 mg/g dry weight. late-season increase...

10.1021/es015702i article EN Environmental Science & Technology 2002-02-19

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is an established technique for the characterization of solid sample surfaces. The introduction polyatomic beams, such as C(60), has provided associated ability to perform molecular depth-profiling and 3D imaging. However, not all samples equally under C(60) bombardment, it probably naïve think that there will be beam applicable in situations. It therefore important explore potential other candidates. A systematic study suitability...

10.1021/ac200288v article EN Analytical Chemistry 2011-04-04

Transflection-mode FTIR spectroscopy has become a popular method of measuring spectra from biomedical and other samples due to the relative low cost substrates compared transmission windows, higher absorbance double pass through same sample approximately doubling effective path length. In this publication we state an optical description on multilayer low-e reflective substrates. Using model are able explain in detail so-called electric-field standing wave effect rationalise non-linear change...

10.1039/c2an36090j article EN The Analyst 2012-10-26

Time‐of‐flight secondary ion mass spectrometry (ToFSIMS) is being applied increasingly to the study of biological systems where chemical specificity and high lateral resolution imaging capabilities can be exploited. Here we report a comparison two cell sample preparation methods demonstrate how they influence outcome ToFSIMS analysis for three‐dimensional (3D) cells using our novel buncher‐ToF instrument (J105 3D Chemical Imager) equipped with C 60 primary beam. Cells were analysed fixed...

10.1002/rcm.4944 article EN Rapid Communications in Mass Spectrometry 2011-03-14

Low secondary ion yields from organic and biological molecules are the principal limitation on future exploitation of time flight-secondary mass spectrometry (TOF-SIMS) as a surface materials analysis technique. On basis hypothesis that increasing density water related fragments in impact zone would enhance proton mediated reactions, prototype cluster beam has been developed using supersonic jet expansion methodologies enable 10 keV (H2O)1000+ to be compared with those obtained Ar1000+ beam....

10.1021/ac4013732 article EN cc-by Analytical Chemistry 2013-05-29

Breast cancer is an umbrella term used to describe a collection of different diseases with broad inter- and intratumor heterogeneity. Understanding this variation critical in order develop, precisely prescribe, new treatments. Changes the lipid metabolism cancerous cells can provide important indications as metabolic state but are difficult investigate conventional histological methods. Due introduction higher energy (40 kV) gas cluster ion beams (GCIBs), time-of-flight secondary mass...

10.1021/acs.analchem.6b03884 article EN cc-by-nc-nd Analytical Chemistry 2016-10-26

Peptide or protein structural analysis is crucial for the evaluation of biochips and biodevices, therefore an analytical technique with ability to detect identify peptide species directly from surfaces high lateral resolution required. In this report, efficacy ToF-SIMS analyze proteins evaluated. Although physics governing SIMS bombardment process precludes researchers intact larger peptides greater than a few thousand mass unit directly, it possible obtain information on partial structures...

10.1021/acs.analchem.5b04133 article EN Analytical Chemistry 2016-02-26

There is emerging evidence that amyloid beta (Aβ) aggregates forming neuritic plaques lead to impairment of the lipid-rich myelin sheath and glia. In this study, we examined focal lipid alterations disruption associated with in a widely used familial Alzheimer's disease (AD) mouse model; 5xFAD. This AD model has Aβ42 peptide-rich plaque deposition brain parenchyma. Matrix-assisted laser desorption/ionization imaging mass spectrometry coronal tissue sections revealed Aβ plaque-associated...

10.1111/jnc.14999 article EN cc-by Journal of Neurochemistry 2020-03-06

ADVERTISEMENT RETURN TO ISSUEPREVArticlePotential environmental risks associated with the new sulfonylurea herbicidesJohn S. Fletcher, Thomas G. Pfleeger, and Hilman C. RatschCite this: Environ. Sci. Technol. 1993, 27, 10, 2250–2252Publication Date (Print):September 1, 1993Publication History Published online1 May 2002Published inissue 1 September 1993https://pubs.acs.org/doi/10.1021/es00047a037https://doi.org/10.1021/es00047a037research-articleACS PublicationsRequest reuse...

10.1021/es00047a037 article EN Environmental Science & Technology 1993-09-01
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