A5062883841- Ion-surface interactions and analysis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Analytical chemistry methods development
- Mass Spectrometry Techniques and Applications
- Integrated Circuits and Semiconductor Failure Analysis
- Forensic Fingerprint Detection Methods
- Spectroscopy Techniques in Biomedical and Chemical Research
- Diamond and Carbon-based Materials Research
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Nonmelanoma Skin Cancer Studies
- Cancer and Skin Lesions
- Hydrogen embrittlement and corrosion behaviors in metals
- Bacterial Identification and Susceptibility Testing
- Analytical Chemistry and Sensors
- Cutaneous Melanoma Detection and Management
- Crystallography and molecular interactions
- Force Microscopy Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- Luminescence and Fluorescent Materials
- Advanced Biosensing Techniques and Applications
Seikei University
2016-2025
Sony (Taiwan)
2022
Panasonic (Japan)
2020
Toray Industries, Inc. (Japan)
2020
Hiroshima University
2020
National Institute for Materials Science
2020
Yokkaichi University
2020
Hokkaido University
2004-2015
Shimane University
2005-2014
Tottori University
2011-2014
Hepatitis B e antigen (HBeAg) constitutes the nucleocapsid of hepatitis virus (HBV) and occurs in association with plasma proteins, particularly IgG, serum persons infected virus. A polypeptide an approximate m.w. 15,500 (P15.5) is obtained either from HBeAg or HBV. P15.5 preparations resembled closely each other amino acid composition. The C-terminus sequence was determined to be -Thr-Thr-Val-Val, whereas that ended -Thr-Thr. same four residues found on gene coding for nucleopeptide a...
Peptide or protein structural analysis is crucial for the evaluation of biochips and biodevices, therefore an analytical technique with ability to detect identify peptide species directly from surfaces high lateral resolution required. In this report, efficacy ToF-SIMS analyze proteins evaluated. Although physics governing SIMS bombardment process precludes researchers intact larger peptides greater than a few thousand mass unit directly, it possible obtain information on partial structures...
We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) interlaboratory study measurement composition in organic depth profiling. Layered samples with known binary compositions Irganox 1010 either 1098 or Fmoc-pentafluoro-l-phenylalanine each layer were manufactured single batch distributed to more than 20 participating laboratories. The analyzed using argon cluster ion sputtering X-ray photoelectron spectroscopy (XPS) time-of-flight secondary mass...
Hidradenitis suppurativa (HS) is a chronic follicular occlusive disease with characteristic recurrent draining sinuses, skin abscesses and disfiguring scars, mainly involving the axilla, groin, perianal perineal regions. While most HS cases are nonfamilial, familial showing autosomal dominant inheritance have been reported. Recently, loss-of-function mutations in genes encoding γ-secretase identified as cause of Chinese British populations. To identify Japanese patients nonfamilial HS. Two...
The interpretation of time-of-flight secondary ion mass spectrometry (ToF-SIMS) data is often complicated because ToF-SIMS has a high sensitivity for detecting extremely low amounts molecules and generally produces numerous types fragment ions from each molecule. Although machine learning techniques have been applied to such complex classify the components in sample, identifying unknown difficult, even after classification or segmentation datasets. We developed new (SIMS) identification...
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful tool for determining surface information of complex systems such as polymers and biological materials. However, the interpretation ToF-SIMS raw data often difficult. Multivariate analysis has become effective methods data. Some multivariate principal component curve resolution are useful simplifying consisting many components to that explained by smaller number components. In this study, four layers three was analyzed...
We report a case of invasive SCC arising from multiple lesions Bowen's disease with right inguinal lymph-node metastasis. Assessment superficial involvement by real-time tissue elastography before surgery was found to be more useful than other noninvasive conventional methods. Histologically, the metastatic tumour cells were located asymmetrically in small section cortical area node, and this result comparable elastographic findings. Additionally, we that presence an asymmetrical high...
The outermost surface of the human hair is covered with a thin layer fatty acids bonding proteins, which plays an important role in giving soft and smooth feeling hydrophobicity. In this study, changes protein fragment ions were characterized using time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) X‐ray photoelectron spectroscopy (XPS) to investigate damage. As result, intensity m / z 76 related cystine cysteine, major components keratin, decreased damage, consistent generation...
We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) interlaboratory study identification peptide sample TOF-SIMS spectra by machine learning. More than 1000 time-of-flight secondary ion mass spectrometry (TOF-SIMS) six model samples (one them was test sample) were collected using 27 instruments from 25 institutes countries, U. S., K., Germany, China, South Korea, Japan. Because peptides have systematic simple chemical structures, they selected as...
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is extensively employed for the structural analysis of outermost surfaces organic materials, including biological because it provides detailed compositional information and enables high-spatial-resolution chemical mapping. In this study, a combination TOF-SIMS data was to evaluate materials composed numerous proteins, unknown ones. To interpret complicated human hair, an autoencoder, dimensionality reduction method based on artificial...
Abstract The dynamics of hydrogen in metals with mixed grain structure is not well understood at a microscopic scale. One the biggest issues facing economy “hydrogen embrittlement” metal induced by entering and diffusing into material. Hydrogen diffusion metallic materials difficult to grasp owing non-uniform compositions structures metal. Here time-resolved “operando microscope” was used interpret local behaviour microstructure stainless steel austenite martensite structures....
Recessive dystrophic epidermolysis bullosa (RDEB) is an inherited skin disorder caused by mutations in the COL7A1 gene, which encodes collagen VII (COL7). Skin ulcers RDEB patients are sometimes slow to heal. We describe here therapeutic response of intractable two with generalized treatment allogeneic cultured dermal substitute (CDS). both epithelialized 3–4 weeks after this treatment. Immunohistochemical studies demonstrated that COL7 expression level remained reduced respect control and...
Abstract The evaluation of proteins on biodevices, such as the distribution or orientation immobilized proteins, is one most important issues for development sophisticated biodevices. In this review, a description provided application sensitive surface analysis methods, time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS), to protein and TOF‐SIMS spectra using mutual information. useful biodevice surfaces, because provides submicron‐scale chemical mapping information structures upper...
Abstract Background Patients with in situ extramammary Paget's disease ( EMPD ) tend to have a good prognosis, although dermal invasion and metastasis are associated significantly increased morbidity mortality. Previous studies addressed mechanisms underlying the pathogenesis; however, no molecular markers that reflect invasiveness or progression been established. Objective This study aims identify reliable marker for predicting risk of . Methods We performed an initial microarray screening...
Matrix effects are crucial for analyses using time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) in terms of quantitative analysis, depth profiling and imaging. It is often difficult to predict how co‐existing materials will influence each other before such analysis. However, matrix need be curtailed order assume the appropriate amount a target material sample. First, on different types organic mixed samples, including sample composed Irganox 1010 1098 (MMK sample) another...
In this study, the authors evaluated distribution of low-abundance beauty ingredients in human skin tissues. The collagen tripeptide, a ingredient, was by applying multivariate curve resolution (MCR) to time-of-flight secondary ion mass spectrometry mapping data, including reference information. intensity peaks increased accumulation depth direction obtained argon cluster sputtering. Consequently, tripeptide separating it from peptide, although conventional analysis difficult because...
Machine learning is a useful tool when extracting hidden information from complex measurement data obtained via surface analysis, as in secondary ion mass spectrometry. Flexible methods often require significant effort to adjust parameters, these parameters may have effect on results. However, machine enable the extraction of new that cannot be found by manual analysis. This paper presents some examples analyses using conventional multivariate analysis based linear combinations (principal...
The rare presence of proliferative nodules in cases giant congenital naevus can, some cases, be potentially misdiagnosed as neonatal melanoma. We report here a case multiple, found lesion female patient diagnosed with neurocutaneous melanosis. Our initial clinical observations this suggested the possibility primary cutaneous melanoma or skin metastasis from meninges elsewhere central nervous system. However, histological examination revealed no sign melanoma, abnormal mitosis, necrosis any...