A5038144878- Ion-surface interactions and analysis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Mass Spectrometry Techniques and Applications
- Diamond and Carbon-based Materials Research
- Advanced Proteomics Techniques and Applications
- Forensic Fingerprint Detection Methods
- Analytical chemistry methods development
- Metabolomics and Mass Spectrometry Studies
- Water Quality Monitoring and Analysis
- Geomechanics and Mining Engineering
- Ferroelectric and Piezoelectric Materials
- Nanopore and Nanochannel Transport Studies
- Acoustic Wave Resonator Technologies
- Rock Mechanics and Modeling
- Integrated Circuits and Semiconductor Failure Analysis
- Ultrasound Imaging and Elastography
- Synthesis and Characterization of Heterocyclic Compounds
- Trace Elements in Health
- Advanced Battery Materials and Technologies
- Ultrasonics and Acoustic Wave Propagation
- Nuclear Physics and Applications
- Nanoparticles: synthesis and applications
- Advanced Battery Technologies Research
- Geological and Geophysical Studies
Yan'an University
2025
University of Pittsburgh
2022-2024
Shaanxi Normal University
2007-2024
National University of Singapore
2024
Pennsylvania State University
2014-2023
Southern Medical University Shenzhen Hospital
2021
Third Affiliated Hospital of Southern Medical University
2021
Zhengzhou University
2018-2019
Dongfeng Motor Group (China)
2018
China University of Mining and Technology
2009-2018
Metabolons, multiprotein complexes consisting of sequential enzymes a metabolic pathway, are proposed to be biosynthetic "hotspots" within the cell. However, experimental demonstration their presence and functions has remained challenging. We used metabolomics in situ three-dimensional submicrometer chemical imaging single cells by gas cluster ion beam secondary mass spectrometry (GCIB-SIMS) directly visualize de novo purine biosynthesis multienzyme complex, purinosome. found that...
Abstract The development of glioblastoma (GBM) is typically accompanied by marked changes in lipid metabolism. Oxylipins and their catalyzed enzymes lipoxygenases (LOXs) have been shown to participate the cancers via multiple pathways, while understanding LOXs GBM remains enigmatic. Thus, we aimed explore expression functional roles GBM. Here showed that ALOXE3 was markedly down-regulated human Knockdown cells fostered orthotopic tumor growth shortened lifespan mice. deficiency rendered...
Growing cancer cells effectively evade most programs of regulated cell death, particularly apoptosis. This necessitates a search for alternative therapeutic modalities to cause cell's demise, among them – ferroptosis. One the obstacles using pro-ferroptotic agents treat is lack adequate biomarkers Ferroptosis accompanied by peroxidation polyunsaturated species phosphatidylethanolamine (PE) hydroperoxy- (-OOH) derivatives, which act as death signals. We demonstrate that RSL3-induced A375...
Abstract Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances field now allow high-parameter data collection (>60 targets); however, considerable expertise capital are needed to construct antibody panels employed by these methods. Organ mapping community-validated resources that save time money, increase reproducibility, accelerate discovery support construction a Human Reference Atlas.
The inherent difficulty of discovering new and effective antibacterials the rapid development resistance particularly in Gram-negative bacteria, illustrates urgent need for methods that enable rational drug design. Here we report 3D imaging cluster Time-of-Flight secondary ion mass spectrometry (ToF-SIMS) as a label-free approach to chemically map small molecules aggregated single Escherichia coli cells, with ∼300 nm spatial resolution high chemical sensitivity. feasibility quantitative...
Gas cluster ion beam-secondary mass spectrometry (GCIB-SIMS) has shown the full potential of mapping intact lipids in biological systems with better than 10 μm lateral resolution. This study investigated further capability GCIB-SIMS imaging high-mass signals from cardiolipin (CL) and gangliosides normal brain effect a controlled cortical impact model (CCI) traumatic injury (TBI) on their distribution. A combination enzymatic chemical treatments was employed to suppress most abundant...
Abstract Millions of diverse molecules constituting the lipidome act as important signals within cells. Of these, cardiolipin (CL) and phosphatidylethanolamine (PE) participate in apoptosis ferroptosis, respectively. Their subcellular distribution is largely unknown. Imaging mass spectrometry capable deciphering spatial multiple lipids at levels. Here we report development a unique 70 keV gas‐cluster ion beam that consists (CO 2 ) n + ( >10 000) projectiles. Coupled with direct current...
Abstract Background As one of the major components lignocellulosic biomass, lignin has been considered as most abundant renewable aromatic feedstock in world. Comparing with thermal or catalytic strategies for degradation, biological conversion is a promising approach featuring mild conditions and diversity, received great attention nowadays. Results In this study, consortium white rot fungi composed Lenzites betulina Trametes versicolor was employed to enhance ligninolytic enzyme activity...
Abstract Peroxidized phosphatidylethanolamine (PEox) species have been identified by liquid chromatography mass spectrometry (LC‐MS) as predictive biomarkers of ferroptosis, a new program regulated cell death. However, the presence and subcellular distribution PEox in specific types tissues not directly detected imaging protocols. By applying gas cluster ion beam secondary (GCIB‐SIMS) with 70 keV (H 2 O) n + (n>28 000) beam, we were able to map 1.2 μm spatial resolution at single...
Integration of multiomics at the single-cell level allows unambiguous dissecting phenotypic heterogeneity different states such as health, disease, and biomedical response. Imaging mass spectrometry holds promise being able to measure multiple types biomolecules in parallel same cell. We have explored possibility using water gas cluster ion beam secondary [(H2O)n-GCIB-SIMS] an analytical tool for assay. (H2O)n-GCIB has been hailed ideal ionization source biological sampling owing enhanced...
The temporo-spatial organization of different cells in the tumor microenvironment (TME) is key to understanding their complex communication networks and immune landscape that exists within compromised tissues. Multi-omics profiling single-interacting native TME critical for providing further information regarding reprograming mechanisms leading immunosuppression progression. This requires new technologies biomolecular phenotypically heterogeneous on same tissue sample. Here, we developed a...
We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) interlaboratory study measurement composition in organic depth profiling. Layered samples with known binary compositions Irganox 1010 either 1098 or Fmoc-pentafluoro-l-phenylalanine each layer were manufactured single batch distributed to more than 20 participating laboratories. The analyzed using argon cluster ion sputtering X-ray photoelectron spectroscopy (XPS) time-of-flight secondary mass...
miRNAs are a family of single-stranded non-coding RNAs that regulate gene expression by targeting messenger (mRNAs) for suppression, with an average length 22 nt. The oriental armyworm, Mythimna separata Walker, is pest insect long-distance migratory capability, which causes severe loss grains and pastures in Eastern Asia, Southeastern Oceania. This study aims to elucidate the post-transcriptional regulatory mechanisms development this pest. We carried out small RNA sequencing on samples...
Chemical modification of a rare gas cluster ion beam (GCIB) to increase the intensity desorbed molecular ions in secondary mass spectrometry experiments relative pure Ar cluster.Doping GCIB by mixing small concentration levels (1-3% partial pressure) CH4 into driving source.Mass spectra measured on trehalose film using doped exhibit enhanced signals. From depth profiling experiments, results are shown arise from an ionization efficiency sputtered molecules rather than change sputtering yield...
The emergence of argon-based gas cluster ion beams for SIMS experiments opens new possibilities molecular depth profiling and 3D chemical imaging. These generally leave less surface damage yield mass spectra with reduced fragmentation compared smaller projectiles. For nanoscale bioimaging applications, however, limited sensitivity due to low ionization probability technical challenges beam focusing remain problematic. use based upon systems other than argon offer an opportunity resolve these...
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and early development stages with subcellular lateral resolution (∼4 μm). The method avoids complicated sample preparation lipid embryos, which requires selective chemical extraction a pool samples chromatographic separation, while preserving spatial distribution biological species. results show...
Previous studies have shown that the use of a 20 keV water cluster beam as primary for analysis organic and bio-organic systems resulted in 10–100 times increase positive molecular ion yield range typical analytes compared to C60 argon beams. This increased sensitivity important lipid molecules bioimaging rat brain. Building on these studies, present work compares 40 70 beams with composed pure argon, 10%CO2, CO2. First, previously, we show E/nucleon about 0.3 eV/nucleon nonwater containing...