A5091760297- Advanced MRI Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Single-cell and spatial transcriptomics
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- Biosensors and Analytical Detection
- Health, Environment, Cognitive Aging
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Neurogenetic and Muscular Disorders Research
- Electron Spin Resonance Studies
- Neurofibromatosis and Schwannoma Cases
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Mesoporous Materials and Catalysis
- Ion-surface interactions and analysis
- Catalysis and Hydrodesulfurization Studies
- Protein Tyrosine Phosphatases
- Nanoparticle-Based Drug Delivery
- Lignin and Wood Chemistry
- Diamond and Carbon-based Materials Research
- Advanced Biosensing Techniques and Applications
Northwestern University
2019-2025
Tempus Labs (United States)
2019-2022
Michigan State University
2014-2016
Electrocatalytic hydrogenation (ECH) of guaiacol for production chemical and fuel in a divided cell using earth abundant metal electrodes. Specific energies shown below the organics are their higher heating values (HHV).
Abstract Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances field now allow high-parameter data collection (>60 targets); however, considerable expertise capital are needed to construct antibody panels employed by these methods. Organ mapping community-validated resources that save time money, increase reproducibility, accelerate discovery support construction a Human Reference Atlas.
We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection intact proteins from cells dropcast onto glass slides. Sampling whole proteoforms by individual ion mass spectrometry enable a scalable approach to proteomics. This new scPiMS platform addresses throughput bottleneck in proteomics boosts processing rate several fold while accessing protein composition with higher coverage.
Our lab has developed a new series of self-immolative MR agents for the rapid detection enzyme activity in mouse models expressing β-galactosidase (β-gal). We investigated two molecular architectures to create that detect β-gal by modulating coordination water GdIII . The first is an intermolecular approach, wherein we designed several structural isomers maximize endogenous carbonate ions. second involves intramolecular mechanism q modulation. incorporated pendant coordinating carboxylate...
Abstract The vast complexity of the proteome currently overwhelms any single analytical technology in capturing full spectrum proteoform diversity. In this study, we evaluated complementarity two cutting-edge proteomic technologies—single-molecule protein sequencing and individual ion mass spectrometry—for analyzing recombinant human IL-6 (rhIL-6) at amino acid, peptide, intact levels. For single-molecule sequencing, employ recently released Platinum ® instrument. Next-Generation Protein...
Abstract We introduce single-cell Proteoform imaging Mass Spectrometry ( scPiMS ), which realizes the benefit of direct analysis intact proteins to process 10,836 single cells from rat hippocampus. This new platform addresses throughput bottleneck for cell proteomics using mass spectrometry, boosting processing rates by >20-fold in field. identified 169 proteoforms <70 kDa brain and classified 2758 them as neurons, astrocytes or microglia types.
The dynamic range challenge for the detection of proteins and their proteoforms in human plasma has been well documented. Here, we use nanoparticle protein corona approach to enrich low-abundance selectively reproducibly from top-down proteomics quantify differential enrichment 2841 detected 114 proteins. Furthermore, allowed between ∼1 μg/mL ∼10 pg/mL absolute abundance, providing up a 10
Transformative results of adeno-associated virus (AAV) gene therapy in patients with spinal muscular atrophy and Leber's congenital amaurosis led to approval the first two AAV products United States treat these diseases. These extraordinary a dramatic increase number type gene-therapy programs. However, field lacks non-invasive means assess levels duration therapeutic protein function patients. Here, we describe new magnetic resonance imaging (MRI) technology for real-time reporting living...
Abstract Our lab has developed a new series of self‐immolative MR agents for the rapid detection enzyme activity in mouse models expressing β‐galactosidase (β‐gal). We investigated two molecular architectures to create that detect β‐gal by modulating coordination water Gd III . The first is an intermolecular approach, wherein we designed several structural isomers maximize endogenous carbonate ions. second involves intramolecular mechanism q modulation. incorporated pendant coordinating...
ProGlo is an efficient steroid receptor-targeted magnetic resonance (MR) imaging contrast agent (CA). It has been shown to bind the progesterone receptor (PR) and produce enhanced image in PR-positive cells tissues vitro vivo. However, hydrophobicity of targeting domain (logP = 1.4) limits its formulation delivery at clinically relevant doses. In this work, a hydrophobic moiety was utilized drive adsorption onto nanodiamond (ND) clusters form water-soluble nanoconstruct −2.4) with 80%...
Diagnostic medical imaging utilizes magnetic resonance (MR) to provide anatomical, functional, and molecular information in a single scan. Nanoparticles are often labeled with Gd(III) complexes amplify the MR signal of contrast agents (CAs) large payloads high proton relaxation efficiencies (relaxivity, r1). This study examined performance two structurally unique cages, AaLS-13 OP, Gd(III). The cages have characteristics relevant for development theranostic platforms, including (i)...
The dynamic range challenge for detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundant selectively reproducibly from top-down proteomics quantify differential enrichment 2841 detected 114 proteins. Furthermore, allowed between ∼1 µg/mL ∼10 pg/mL absolute abundance, providing up 10
F parashift probes with paramagnetically shifted reporter nuclei provide attractive platforms to develop molecular imaging probes. These enable ratiometric detection of disease markers using a direct technique. Here, we describe series trivalent lanthanide (Ln(III)) complexes that are structural analogues the clinically approved MR contrast agent (CA) ProHance obtain
The favorable pH response and coordination properties of the 3-hydroxypyridyl group indicate its potential for further development as a dual responsive-reporter Ln(<sc>iii</sc>) MRS probes.
Abstract Sodium metal trapped in the nanoscale pores of alumina gel efficiently reduces Weinreb amides (I) simple batch reaction mode.
Abstract Background: We recently reported two ESR1 fusions (ESR1-YAP1 and ESR1-PCDH11X) that drive endocrine therapy (ET) resistance metastasis in estrogen receptor positive (ER+) metastatic breast cancer (MBC) (PMC6171747). Here we report additional with diverse C-terminal partner genes - ESR1-DAB2, ESR1-GYG1, ESR1-SOX9, ESR1-ARNT2, ESR1-PCMT1 ESR1-ARID1B. Their functional characteristics effects on kinase biology will be described. Methods: were identified by RNA-seq MBC using ChimeraScan...
Abstract BACKGROUND GBM genomic profiling relies on sequencing a limited tumor tissue sample, which is invasive and may underrepresent molecular heterogeneity. CSF, can be obtained less invasively, in intimate contact with lesions better capture the full profile of tumor. However, datasets contemporaneously collected CSF to support this claim have been lacking. To evaluate performance NGS, we conducted pilot study patients undergoing resection for suspected recurrence following first-line...