A5059275651- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Bacterial Identification and Susceptibility Testing
- Cell Image Analysis Techniques
- Medical Imaging Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- AI in cancer detection
- Anatomy and Medical Technology
- Health, Environment, Cognitive Aging
- Pediatric Urology and Nephrology Studies
- Retinal Diseases and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Cancer Genomics and Diagnostics
- Advanced Biosensing Techniques and Applications
- Antimicrobial Resistance in Staphylococcus
- Glycosylation and Glycoproteins Research
- Infective Endocarditis Diagnosis and Management
- MRI in cancer diagnosis
- Connexins and lens biology
- Advanced Electron Microscopy Techniques and Applications
Vanderbilt University
2016-2025
Vanderbilt University Medical Center
2014-2025
Imaging Center
2019-2023
Delft University of Technology
2023
Bruker (United States)
2020
University of Florida
2020
IONICS Mass Spectrometry (Canada)
2015
University of Delaware
2006-2009
College of Wooster
2005
Transformative technologies are enabling the construction of three dimensional (3D) maps tissues with unprecedented spatial and molecular resolution. Over next seven years, NIH Common Fund Human Biomolecular Atlas Program (HuBMAP) intends to develop a widely accessible framework for comprehensively mapping human body at single-cell resolution by supporting technology development, data acquisition, detailed mapping. HuBMAP will integrate its efforts other funding agencies, programs,...
CD8+ T cells are master effectors of antitumor immunity, and their presence at tumor sites correlates with favorable outcomes. However, metabolic constraints imposed by the microenvironment (TME) can dampen ability to control progression. We describe lipid accumulation in TME areas pancreatic ductal adenocarcinoma (PDA) populated infiltrating both murine human tumors. In this lipid-rich but otherwise nutrient-poor TME, access using metabolism becomes particularly valuable for sustaining cell...
Imaging mass spectrometry (IMS) enables the spatially targeted molecular assessment of biological tissues at cellular resolutions. New developments and technologies are essential for uncovering drivers native physiological function disease. Instrumentation must maximize spatial resolution, throughput, sensitivity, specificity, because tissue imaging experiments consist thousands to millions pixels. Here, we report development application a matrix-assisted laser desorption/ionization (MALDI)...
We have achieved enhanced lipid imaging to a ∼10 μm spatial resolution using negative ion mode matrix assisted laser desorption ionization (MALDI) mass spectrometry, sublimation of 2,5-dihydroxybenzoic acid as the MALDI matrix, and sample preparation protocol that uses aqueous washes. report on effect treating tissue sections by washing with volatile buffers at different pHs prior imaging. The results show ammonium formate, pH 6.4, or acetate, 6.7, significantly increases signal intensity...
A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state capable of pulse repetition rates up to 5 kHz and continuous raster sampling high-throughput data collection. Lipid images sagittal rat brain tissue section were collected in 10 min with an effective acquisition rate roughly 30 pixels/s. These results represent more than 10-fold increase...
Non‐small cell lung cancer (NSCLC) is the leading cause of death globally. To develop better diagnostics and more effective treatments, research in past decades has focused on identification molecular changes genome, transcriptome, proteome, recently also metabolome. Phospholipids, which nevertheless play a central role functioning, remain poorly explored. Here, using mass spectrometry (MS)‐based phospholipidomics approach, we profiled 179 phospholipid species malignant matched non‐malignant...
MALDI imaging mass spectrometry is a powerful analytical tool enabling the visualization of biomolecules in tissue. However, there are unique challenges associated with protein experiments including need for higher spatial resolution capabilities, improved image acquisition rates, and better molecular specificity. Here we demonstrate capabilities ultra-high speed MALDI-TOF high FTICR IMS platforms as they relate to these challenges. High images rat brain tissue cystic fibrosis lung were...
MALDI imaging mass spectrometry is a highly sensitive and selective tool used to visualize biomolecules in tissue. However, identification of detected proteins remains difficult task. Indirect strategies have been limited by insufficient accuracy confidently link ion images proteomics data. Here, we demonstrate the capabilities FTICR MS for intact proteins. IMS provides an unprecedented combination resolving power (~75,000 at m/z 5000) (<5ppm) up ~12kDa, enabling based on correlation with...
Diabetic nephropathy (DN) is a major life-threatening complication of diabetes. Renal lesions affect glomeruli and tubules, but the pathogenesis not completely understood. Phospholipids glycolipids are molecules that carry out multiple cell functions in health disease, their role DN unknown. We employed high spatial resolution MALDI imaging MS to determine lipid changes kidneys eNOS(-/-) db/db mice, robust model DN. Phospholipid glycolipid structures, localization patterns, relative tissue...
Matrix assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) has the ability to provide an enormous amount of information on abundances and spatial distributions molecules within biological tissues. The rapid progress in development this technology significantly improves our analyze smaller areas features mammalian eye evolved over millions years become essential asset for survival, providing important sensory input organism's surroundings. highly complex retina is...
Lipids are a structurally diverse class of molecules with important biological functions including cellular signaling and energy storage. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for direct mapping biomolecules in tissues. Fully characterizing the structural diversity lipids remains challenge due to presence isobaric isomeric species, which greatly complicates data interpretation when only m/z information is available. Integrating ion...
Imaging of proteoforms in human tissues is hindered by low molecular specificity and limited proteome coverage. Here, we introduce proteoform imaging mass spectrometry (PiMS), which increases the size limit for detection identification fourfold compared to reported methods reveals tissue localization at <80-μm spatial resolution. PiMS advances combining ambient nanospray desorption electrospray ionization with ion using individual spectrometry. We demonstrate highly multiplexed kidney,...
Bone and bone marrow are vital to mammalian structure, movement, immunity. These tissues also commonly subjected molecular alterations giving rise debilitating diseases like rheumatoid arthritis osteomyelitis. Technologies such as matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) facilitate the discovery of spatially resolved chemical information in biological tissue samples help elucidate complex processes underlying pathology. Traditionally, preparation...
Abstract Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances field now allow high-parameter data collection (>60 targets); however, considerable expertise capital are needed to construct antibody panels employed by these methods. Organ mapping community-validated resources that save time money, increase reproducibility, accelerate discovery support construction a Human Reference Atlas.
Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since tissue blanks do not exist for metabolites, careful consideration must be given to confirm identity. Here, we present approaches the improvement in detection of amine such as amino acids neurotransmitters tissues through chemical derivatization matrix‐assisted laser desorption/ionization (MALDI) IMS. Chemical with...
Siderophores, iron-scavenging small molecules, are fundamental to bacterial nutrient metal acquisition and enable pathogens overcome challenges imposed by nutritional immunity. Multimodal imaging mass spectrometry allows visualization of host-pathogen iron competition, mapping siderophores within infected tissue. We have observed heterogeneous distributions Staphylococcus aureus across infectious foci, challenging the paradigm that vertebrate host is a uniformly iron-depleted environment...