A5018107375- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Cell Adhesion Molecules Research
- Pulmonary Hypertension Research and Treatments
- Epigenetics and DNA Methylation
- Medical Imaging and Pathology Studies
- Childhood Cancer Survivors' Quality of Life
- Single-cell and spatial transcriptomics
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Extracellular vesicles in disease
- Cellular Mechanics and Interactions
- Blood properties and coagulation
- TGF-β signaling in diseases
- Peptidase Inhibition and Analysis
- Occupational exposure and asthma
- Tracheal and airway disorders
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Health, Environment, Cognitive Aging
- Occupational and environmental lung diseases
- Protease and Inhibitor Mechanisms
- Immune Response and Inflammation
- Fibroblast Growth Factor Research
- NF-κB Signaling Pathways
University of North Carolina at Chapel Hill
2018-2025
Lung Institute
2022-2024
Interstitial Cystitis Association
2013-2024
Cohen Children's Medical Center
2023
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2023
Children's Mercy Hospital
2023
University of North Carolina System
2023
Schneider Children's Hospital
2023
Children's Hospital of Pittsburgh
2023
Stanford University
2023
Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations individual the variability present in population. Here we integrated Human Lung Cell Atlas (HLCA), combining 49 respiratory system into single atlas spanning over 2.4 million cells from 486 individuals. The HLCA presents consensus re-annotation with...
Transformative technologies are enabling the construction of three dimensional (3D) maps tissues with unprecedented spatial and molecular resolution. Over next seven years, NIH Common Fund Human Biomolecular Atlas Program (HuBMAP) intends to develop a widely accessible framework for comprehensively mapping human body at single-cell resolution by supporting technology development, data acquisition, detailed mapping. HuBMAP will integrate its efforts other funding agencies, programs,...
Section:ChooseTop of pageAbstract <<Biology Alveolar Injur...Role ECMPreclinical ModelingThe Role Inflammation ...Genetic, Epigenetic, and ...Translation Discoverie...SummaryReferencesCITING ARTICLES
DNA methylation is an important epigenetic mechanism, which often occurs in response to environmental stimuli and crucial regulating gene expression. It likely that alterations contribute pathogenesis idiopathic pulmonary fibrosis (IPF).To determine the changes IPF their effects on expression.Total methyltransferase expression were compared normal control lung tissues. tissues subjected comparative analysis of genome-wide RNA using hybridization Illumina HumanMethylation27 BeadChip...
A subset of individuals who recover from coronavirus disease 2019 (COVID-19) develop post-acute sequelae severe acute respiratory syndrome 2 (SARS-CoV-2) (PASC), but the mechanistic basis PASC-associated lung abnormalities suffers a lack longitudinal tissue samples. The mouse-adapted SARS-CoV-2 strain MA10 produces an distress in mice similar to humans. To investigate PASC pathogenesis, studies MA10-infected were extended clinical recovery phases. At 15 120 days after virus clearance,...
Interleukin-6 (IL-6) and glucocorticoids are important mediators of inflammatory immunological responses. Glucocorticoids known to synergistically enhance IL-6-mediated cellular We now show that IL-6 also has a synergistic effect upon glucocorticoid signaling. In particular, IL-6-activated STAT3 associates with ligand-bound receptor form transactivating/signaling complex, which can function through either an IL-6-responsive element or glucocorticoid-responsive element. These findings reveal...
Mechanisms regulating myofibroblastic differentiation of fibroblasts within fibroblastic foci in idiopathic pulmonary fibrosis (IPF) remain unclear. Epigenetic processes, including DNA methylation, produce heritable but potentially reversible changes or its associated proteins and are prominent development oncogenesis. We have shown that Thy-1 suppresses lung Thy-1(-). down-regulation has been demonstrated cellular transformation clinical cancer. hypothesized epigenetic regulation affects...
Idiopathic pulmonary fibrosis (IPF) is a fatal disease, and therapeutic agents have shown only modest efficacy. Epigenetic alterations contribute to the pathogenesis of IPF. The histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), has been approved for clinical use in cancer; however, its potential efficacy modulating fibroblast survival lung not extensively investigated. We investigated effects SAHA on apoptosis primary IPF myofibroblasts injury-induced murine model....
Fibrosis is characterized by persistent deposition of extracellular matrix (ECM) fibroblasts. Fibroblast mechanosensing a stiffened ECM hypothesized to drive the fibrotic program; however, spatial distribution mechanics and their derangements in progressive fibrosis are poorly characterized. Importantly, presents with significant histopathological heterogeneity at microscale. Here, we report that fibroblastic foci (FF), regions active fibrogenesis idiopathic pulmonary (IPF), surprisingly...
Thy-1 is a glycosylphosphatidyl-inositol-linked cell surface glycoprotein whose exact biological role remains unclear. Differential expression of affects fibroblast proliferation and fibrogenic signaling. In idiopathic pulmonary fibrosis, the proliferating myofibroblasts within fibroblastic foci are Thy-1(-), whereas normal lung fibroblasts predominantly Thy-1(+). this study, we used rat sorted for to examine myofibroblastic differentiation in response stimuli. We examined effects...
Myofibroblasts, key effector cells in tissue fibrosis, are specialized contractile cells. Lung myofibroblast contraction induces integrin alpha(v)beta(5)-dependent latent transforming growth factor (TGF)-beta1 activation suggests that contractility may be a driving force for the persistent differentiation observed fibrotic lungs. Understanding mechanisms regulate fibroblast and mechanotransduction will add new insights into pathogenesis of lung fibrosis lead to therapeutic approaches...
Background: Epidemiologic data from Asia have documented the rapid and extensive emergence of macrolide resistance in Mycoplasma pneumoniae. This drug has also been Europe recently United States, but there is very little information currently available on its prevalence. A technique to identify macrolide-resistant M. pneumoniae needed guide management patients with community-acquired respiratory infections. Methods: Culture Minimum Inhibitory Concentration testing identified infection 2...