A5029268728- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- interferon and immune responses
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bacillus and Francisella bacterial research
- CRISPR and Genetic Engineering
- Biosimilars and Bioanalytical Methods
- Respiratory viral infections research
- Cell Image Analysis Techniques
- Mycobacterium research and diagnosis
- Dengue and Mosquito Control Research
- HIV Research and Treatment
- Virus-based gene therapy research
- Parasitic Infections and Diagnostics
- Adenosine and Purinergic Signaling
- Genetic Neurodegenerative Diseases
- Viral gastroenteritis research and epidemiology
- Amoebic Infections and Treatments
- Viral Infections and Outbreaks Research
University of North Carolina at Chapel Hill
2021-2024
UCLouvain Saint-Louis Brussels
2023
Saint Louis University
2023
Max Planck Institute for Biology
2023
Fred Hutch Cancer Center
2023
Public Health Department
2022
Creighton University
2018
A subset of individuals who recover from coronavirus disease 2019 (COVID-19) develop post-acute sequelae severe acute respiratory syndrome 2 (SARS-CoV-2) (PASC), but the mechanistic basis PASC-associated lung abnormalities suffers a lack longitudinal tissue samples. The mouse-adapted SARS-CoV-2 strain MA10 produces an distress in mice similar to humans. To investigate PASC pathogenesis, studies MA10-infected were extended clinical recovery phases. At 15 120 days after virus clearance,...
The coronavirus disease 2019 (COVID-19) pandemic remains uncontrolled despite the rapid rollout of safe and effective severe acute respiratory syndrome 2 (SARS-CoV-2) vaccines, underscoring need to develop highly antivirals. In setting waning immunity from infection vaccination, breakthrough infections are becoming increasingly common treatment options remain limited. addition, emergence SARS-CoV-2 variants concern, with their potential escape neutralization by therapeutic monoclonal...
Despite the wide availability of several safe and effective vaccines that prevent severe COVID-19, persistent emergence acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VOCs) can evade vaccine-elicited immunity remains a global health concern. In addition, SARS-CoV-2 VOCs therapeutic monoclonal antibodies underscores need for additional, variant-resistant treatment strategies. Here, we characterize antiviral activity GS-5245, obeldesivir (ODV), an oral prodrug parent...
Significance A potential outbreak of swine acute diarrhea syndrome coronavirus (SADS-CoV) in the human population could be devastating. Using genomewide CRISPR knockout screening, we identified placenta-associated 8 protein (PLAC8) as an essential host factor for SADS-CoV infection, uncovering a novel antiviral target CoV infection. The PLAC8-related pathway may also have implications other infections. Given ability to infect primary cultures without adaptation, our findings lay foundation...
Infectious diseases have shaped the human population genetic structure, and variation influences susceptibility to many viral diseases. However, a variety of challenges made implementation traditional Genome-wide Association Studies (GWAS) approaches study these infectious outcomes challenging. In contrast, mouse models provide an experimental control precision, which facilitates analyses mechanistic studies role on infection. Here we use mapping cross between two distinct Collaborative...
Abstract The four dengue virus serotypes co-circulate globally and cause significant human disease. Dengue vaccine development is challenging because some virus-specific antibodies are protective, while others implicated in enhanced viral replication more severe Current tetravalent vaccines contain live attenuated formulated to theoretically induce balanced protective immunity. Among the number of candidates clinical trials, only Dengvaxia licensed for use DENV seropositive individuals. To...
Mature DENVs represent the dominant form in vivo and are target for vaccine development. Here, we used multiple strategies, including protein engineering natural directed evolution to generate DENV1, -2, -3, -4 variants that highly mature without compromising replication efficiency compared parental strains.
COVID-19 survivors develop post-acute sequelae of SARS-CoV-2 (PASC), but the mechanistic basis PASC-associated lung abnormalities suffers from a lack longitudinal samples. Mouse-adapted MA10 produces an acute respiratory distress syndrome (ARDS) in mice similar to humans. To investigate PASC pathogenesis, studies MA10-infected were extended disease through clinical recovery. At 15-120 days post-virus clearance, histologic evaluation identified subpleural lesions containing collagen,...
Despite the wide availability of several safe and effective vaccines that can prevent severe COVID-19 disease, emergence SARS-CoV-2 variants concern (VOC) partially evade vaccine immunity remains a global health concern. In addition, highly mutated neutralization-resistant VOCs such as BA.1 BA.5 or fully (1) many therapeutic monoclonal antibodies in clinical use underlines need for additional treatment strategies. Here, we characterize antiviral activity GS-5245, Obeldesivir (ODV), an oral...
A hallmark of dengue virus (DENV) pathogenesis is the potential for antibody-dependent enhancement, which associated with deadly DENV secondary infection, complicates identification correlates protection, and negatively impacts safety efficacy vaccines. Antibody-dependent enhancement linked to antibodies targeting fusion loop (FL) motif envelope protein, completely conserved in mosquito-borne flaviviruses required viral entry fusion. In current study, we utilized saturation mutagenesis...
A hallmark of dengue virus (DENV) pathogenesis is the potential for antibody-dependent enhancement, which associated with deadly DENV secondary infection, complicates identification correlates protection, and negatively impacts safety efficacy vaccines. Antibody-dependent enhancement linked to antibodies targeting fusion loop (FL) motif envelope protein, completely conserved in mosquito-borne flaviviruses required viral entry fusion. In current study, we utilized saturation mutagenesis...
Infectious diseases have shaped the human population genetic structure, and variation influences susceptibility to many viral diseases. However, a variety of challenges made implementation traditional Genome-wide Association Studies (GWAS) approaches study these infectious outcomes challenging. In contrast, mouse models provide an experimental control precision, which facilitates analyses mechanistic studies role on infection. Here we use mapping cross between two distinct Collaborative...
Abstract Maturation of Dengue viruses (DENV) alters the structure, immunity and infectivity virion highly mature particles represent dominant form in vivo . The production virions principally relies on structure function viral premature protein (prM) its cleavage by host protease furin. We developed a reliable clonal cell line which produces single-round DENVs without need for DENV reverse genetics. More importantly, using engineering coupled with natural directed evolution prM site, we...
ABSTRACT A hallmark of Dengue virus (DENV) pathogenesis is the potential for antibody-dependent enhancement, which associated with deadly DENV secondary infection, complicates identification correlates protection, and negatively impacts safety efficacy vaccines. ADE linked to antibodies targeting fusion loop (FL) motif envelope protein, completely conserved in mosquito-borne flaviviruses required viral entry fusion. In current study, we utilized saturation mutagenesis directed evolution...
A hallmark of Dengue virus (DENV) pathogenesis is the potential for antibody-dependent enhancement, which associated with deadly DENV secondary infection, complicates identification correlates protection, and negatively impacts safety efficacy vaccines. Antibody-dependent enhancement linked to antibodies targeting fusion loop (FL) motif envelope protein, completely conserved in mosquito-borne flaviviruses required viral entry fusion. In current study, we utilized saturation mutagenesis...
A hallmark of Dengue virus (DENV) pathogenesis is the potential for antibody-dependent enhancement, which associated with deadly DENV secondary infection, complicates identification correlates protection, and negatively impacts safety efficacy vaccines. ADE linked to antibodies targeting fusion loop (FL) motif envelope protein, completely conserved in mosquito-borne flaviviruses required viral entry fusion. In current study, we utilized saturation mutagenesis directed evolution engineer a...