A5011527418- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Extracellular vesicles in disease
- Medical Imaging and Pathology Studies
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Tracheal and airway disorders
- Venous Thromboembolism Diagnosis and Management
- Advanced X-ray and CT Imaging
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Renal and related cancers
- Ubiquitin and proteasome pathways
- Iron Metabolism and Disorders
- NF-κB Signaling Pathways
- Diabetes and associated disorders
- TGF-β signaling in diseases
- Cardiac tumors and thrombi
- Pulmonary Hypertension Research and Treatments
- S100 Proteins and Annexins
University Hospital Ulm
2019-2025
Ludwig-Maximilians-Universität München
2022-2025
German Center for Lung Research
2022-2025
First Affiliated Hospital of Henan University
2024
Helmholtz Zentrum München
2022-2024
Klinikum rechts der Isar
2019
Wuxi People's Hospital
2018
Nanjing Medical University
2018
Xiamen University
2015
Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations individual the variability present in population. Here we integrated Human Lung Cell Atlas (HLCA), combining 49 respiratory system into single atlas spanning over 2.4 million cells from 486 individuals. The HLCA presents consensus re-annotation with...
Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with single-cell RNA sequencing induced multilineage circuit fibrogenic cell states hPCLS. We showed that these were highly similar to multicohort atlas from patients pulmonary...
To date, the molecular mechanism underlying constitutive signal transducer and activator of transcription 3 (STAT3) activation in gliomas is largely unclear. In this study, we report that Smad6 overexpressed nuclei glioma cells, which correlates with poor patient survival regulates STAT3 activity via negatively regulating Protein Inhibitors Activated (PIAS3). Mechanically, interacts directly PIAS3, interaction mediated through Mad homology 2 (MH2) domain Ring PIAS3. recruits Smurf1 to...
Hepatocellular carcinoma (HCC) remains the most prevalent type of primary liver cancer worldwide. p53 is one frequently mutated tumor-suppressor genes in HCC and its deficiency hepatocytes triggers tumor formation mice. To investigate iron metabolism during carcinogenesis, we employed a model chronic carbon tetrachloride injections liver-specific p53-deficient mice to induce fibrosis, cirrhosis subsequent carcinogenesis. A transcriptome analysis was identify p53-dependent gene expression...
Alveolar regeneration requires the coordinated engagement of epithelial stem cells and mesenchymal niche to restore intricate alveolar architecture lung. The current paradigm is that certain aspects lung organogenesis are mimicked during injury repair in adult stage. Here, we employ a longitudinal single-cell transcriptomic survey fate map throughout development adulthood. We show myofibroblasts reminiscent developmental (AMFs), termed AMF-like cells, activated following...
The aim of the present study was to investigate correlation between protein expression A-kinase anchor 95 (AKAP95), cyclin D3 and AKT with pathological indicators in lung cancer tissues. Immunohistochemistry used detect levels proteins 51 tissue samples 15 pericarcinoma samples. percentage positive tissues 68.63 28.57%, respectively, difference statistically significant (P<0.01). However, not shown correlate differentiation grade, histological type or lymph node metastasis. In addition,...
ABSTRACT Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with scRNAseq induced multi-lineage circuit fibrogenic cell states hPCLS, which show to be highly similar multi-cohort atlas from pulmonary patients. Using micro-CT staged...
Background: Most liver tumors arise on the basis of chronic diseases that trigger inflammatory responses. Besides inflammation, subsequent defects in p53-signaling pathway frequently occurs cancer. In this study, we analyzed consequences inflammation and p53 loss carcinogenesis. Methods: We used inducible liver-specific transgenic mouse strains to analyze NF-κB/p65 activation mimicking loss. Results: Ikk2ca driven mice results fibrosis, formation ectopic lymphoid structures carcinogenesis...
<title>Abstract</title> Chronic gastritis (CG) is a type of chronic inflammatory response the gastric mucosa. Qingwei San mainly used to treat accumulation heat in stomach and upward attack fire, which has effect clearing cooling blood. This prescription clinically effective treatment gastritis. However, its mechanism action not fully understood needs further study. To explore compounds potential targets molecular (QWS) on gastritis, main active components QWS were obtained through TCMSP,...
This protocol was used to generate spatial proteomics dataset from pediatric lung, as a part of the CZI-MPII project. For more information: Mapping Pediatric Inhalation Interface: Nose, Mouth, and Airways, - Chan Zuckerberg Initiative
<title>Abstract</title> Integration of single cell mRNA sequencing data from millions cells revealed a high diversity types in the healthy and diseased human lung. In large complex organ, which is also constantly exposed to external agents, it crucial understand influence lung tissue topography or factors on gene expression variability within each type. Here, we applied three spatial transcriptomics approaches, to: (i) localize majority types, including rare epithelial topography, (ii)...
This protocol was used to generate spatial proteomics dataset from pediatric lung, as a part of the CZI-MPII project. For more information: Mapping Pediatric Inhalation Interface: Nose, Mouth, and Airways, - Chan Zuckerberg Initiative
This protocol was used to generate spatial proteomics dataset from pediatric lung, as a part of the CZI-MPII project. For more information: Mapping Pediatric Inhalation Interface: Nose, Mouth, and Airways, - Chan Zuckerberg Initiative
Pulmonary fibrosis (PF) is the shared central pathomechanism across multiple entities of interstitial lung diseases. Studying mechanisms regeneration and fibrogenesis directly in human tissue has been hampered by lack organotypic models analytical techniques. Here, we coupled <i>ex vivo</i> cytokine drug perturbations precision-cut slices (hPCLS) with single-cell RNA sequencing to study early tissue. Comparing hPCLS perturbation data against an <i>in multi-cohort atlas from PF patients...
The pulmonary extracellular matrix (ECM) provides structural integrity and essential mechanical properties such as elastic recoil, but also serves an information-rich signaling template that instructs cell identity activity in health disease. spatial distribution specificity of ECM proteins to the lungs distinct tissue niches is mostly unknown. In this study, we use spatially resolved mass spectrometry-based proteomics define global differences proteomic composition anatomically...
Aging is a major risk factor for lung fibrosis. While bleomycin injury in young mice leads to resolving fibrosis, aged develop non-resolving resembling human disease. Here, we conducted longitudinal single-cell RNA profiling of (3 mo) and (18 after bleomycin-induced lung (days 0/3/10/20/30/37 p.i., n=55 mice), coupled multiplexed immunofluorescence uncover spatially-resolved cellular programs responsible this impaired regeneration. Single-cell analyses over 160,000 cells across 69 cell...
Epithelial-mesenchymal crosstalk is essential in lung development and repair upon injury. Such occurs specific subtissular niches, which niche-specific epithelial cell states interact with their mesenchymal counterparts, either driving alveologenesis or re-epithelialization. IPF characterized by a fulminant profibrotic niche, the fibroblast focus, CTHRC1+ myofibroblasts reside close contact KRT17+/KRT5- basaloid cells. Although it currently unknown how these fibrosis-specific evolve,...
Abstract Organ- and body-scale cell atlases have the potential to transform our understanding of human biology. To capture variability present in population, these must include diverse demographics such as age ethnicity from both healthy diseased individuals. The growth size number single-cell datasets, combined with recent advances computational techniques, for first time makes it possible generate comprehensive large-scale through integration multiple datasets. Here, we integrated Human...
Advanced age is a significant risk factor for chronic lung diseases such as idiopathic pulmonary fibrosis (IPF). IPF debilitating disease featuring non-resolving fibrotic remodeling of the lung. While Bleomycin instillation in young mice leads to resolving fibrosis, old develop thereby mimicking human disease. The molecular and cellular programs underlying this age-related impaired regenerative response are incompletely understood. Here, we performed longitudinal single-cell RNA sequencing...