A5032022921- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Force Microscopy Techniques and Applications
- Metal and Thin Film Mechanics
- Extracellular vesicles in disease
- Occupational and environmental lung diseases
- Lung Cancer Treatments and Mutations
- Acoustic Wave Resonator Technologies
- Occupational exposure and asthma
- Renal and related cancers
- Single-cell and spatial transcriptomics
Helmholtz Zentrum München
2021-2023
German Center for Lung Research
2021-2023
Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with single-cell RNA sequencing induced multilineage circuit fibrogenic cell states hPCLS. We showed that these were highly similar to multicohort atlas from patients pulmonary...
Fibrogenic processes instigate fatal chronic diseases leading to organ failure and death. Underlying biological involve induced massive deposition of extracellular matrix (ECM) by aberrant fibroblasts. We subjected diseased primary human lung fibroblasts an advanced three-dimensional phenotypic high-content assay screened a repurposing drug library small molecules for inhibiting ECM deposition. Fibrotic Pattern Detection Artificial Intelligence identified tranilast as effective inhibitor....
ABSTRACT Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with scRNAseq induced multi-lineage circuit fibrogenic cell states hPCLS, which show to be highly similar multi-cohort atlas from pulmonary patients. Using micro-CT staged...
Pulmonary fibrosis (PF) is the shared central pathomechanism across multiple entities of interstitial lung diseases. Studying mechanisms regeneration and fibrogenesis directly in human tissue has been hampered by lack organotypic models analytical techniques. Here, we coupled <i>ex vivo</i> cytokine drug perturbations precision-cut slices (hPCLS) with single-cell RNA sequencing to study early tissue. Comparing hPCLS perturbation data against an <i>in multi-cohort atlas from PF patients...
Idiopathic pulmonary fibrosis (IPF) represents a progressive interstitial lung disease with mean survival time of 3-5 years. Approved pharmacological therapies may slow down, but not stop progression. IPF pathogenesis is characterized by the deposition excessive amounts fibrotic extracellular matrix activated myofibroblasts resulting in loss function. We developed phenotypic drug discovery assay measuring ECM primary human fibroblasts (pHLFs) derived from patients treated transforming growth...