A5089300962- COVID-19 Clinical Research Studies
- Fern and Epiphyte Biology
- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Nematode management and characterization studies
- Genetics, Aging, and Longevity in Model Organisms
- Hydrocarbon exploration and reservoir analysis
- Climate Change and Health Impacts
- Cancer Cells and Metastasis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Respiratory Support and Mechanisms
- Lipid metabolism and disorders
- Multiple Myeloma Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Angiogenesis and VEGF in Cancer
- Prion Diseases and Protein Misfolding
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Adipose Tissue and Metabolism
- Parasite Biology and Host Interactions
- Seed Germination and Physiology
- Plant Diversity and Evolution
- Plant and animal studies
Da Nang University of Technology
2024-2025
National Cancer Center
2025
Translational Genomics Research Institute
2019-2024
Creighton University
2022-2023
White Memorial Medical Center
2022
Loma Linda University
2022
University of Houston
2019-2020
Indiana University Bloomington
2018-2019
University of Iowa
2012-2018
Donald Danforth Plant Science Center
2015
Single-cell RNA sequencing provides new insights into pathologic epithelial and mesenchymal remodeling in the human lung.
Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations individual the variability present in population. Here we integrated Human Lung Cell Atlas (HLCA), combining 49 respiratory system into single atlas spanning over 2.4 million cells from 486 individuals. The HLCA presents consensus re-annotation with...
Cellular diversity of the lung endothelium has not been systematically characterized in humans. We provide a reference atlas human endothelial cells (ECs) to facilitate better understanding phenotypic and composition comprising endothelium.We reprocessed control single-cell RNA sequencing (scRNAseq) data from 6 datasets. EC populations were through iterative clustering with subsequent differential expression analysis. Marker genes validated by fluorescent microscopy situ hybridization....
ABSTRACT The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, creates an urgent need for identifying molecular mechanisms that mediate viral entry, propagation, and tissue pathology. Cell membrane bound angiotensin-converting enzyme 2 (ACE2) associated proteases, transmembrane protease serine (TMPRSS2) Cathepsin L (CTSL), were previously identified as mediators of SARS-CoV2 cellular entry. Here, we assess cell type-specific RNA expression ACE2 , TMPRSS2 CTSL through integrated...
Asexual reproduction is widespread in land plants, including ferns where 10% of all species are obligate asexuals. In these ferns, apogamous sporophytes generated directly from gametophytes, bypassing fertilization. the model fern Ceratopteris richardii, a sexual species, apogamy can be induced by culture on high sugar media. BABY BOOM (BBM) genes angiosperms known to promote somatic embryogenesis, which like produce without Here, Brassica napus BBM (BnBBM) was used investigate genetic...
Abstract Patients with chronic lung disease (CLD) have an increased risk for severe coronavirus disease-19 (COVID-19) and poor outcomes. Here, we analyze the transcriptomes of 611,398 single cells isolated from healthy CLD lungs to identify molecular characteristics that may account worse COVID-19 outcomes in patients diseases. We observe a similar cellular distribution relative expression SARS-CoV-2 entry factors control lungs. AT2 express higher levels genes linked directly efficiency...
Abstract Polyphenism, the extreme form of developmental plasticity, is ability a genotype to produce discrete morphologies matched alternative environments. Because polyphenism likely be under switch-like molecular control, comparative genetic approach could reveal targets plasticity evolution. Here we report that lineage-specific sulfotransferase SEUD-1, which responds environmental cues, dosage-dependently regulates mouthparts in nematode Pristionchus pacificus . SEUD-1 expressed cells...
Ferns, being vascular yet seedless, present unparalleled opportunities to investigate important questions regarding the evolution and development of land plants. Ceratopteris richardii, a diploid, homosporous fern has been advanced as model system; however, tenuous ability transform genome this greatly limited its usefulness organism. Here we report simple reliable Agrobacterium-mediated method for generating transient stable transformants mature C. richardii gametophytes. Transformation...
Abstract Pulmonary fibrosis is a form of chronic lung disease characterized by pathologic epithelial remodeling and accumulation extracellular matrix. In order to comprehensively define the cell types, mechanisms mediators driving fibrotic in lungs with pulmonary fibrosis, we performed single-cell RNA-sequencing suspensions from 10 non-fibrotic control 20 PF lungs. Analysis 114,396 cells identified 31 distinct types. We report remarkable shift phenotypes occurs peripheral PF, identify...
Glioblastoma (GBM) is the most aggressive and lethal primary brain tumor. The prognosis for patients diagnosed with GBM remains very poor median survival time of only 8 months if nothing done, 12 - 15 surgery, radiotherapy, chemotherapy are carried out. This therapeutic outcome abysmal; hence, many new modalities being investigated especially in combination standard care. Nanoparticle-mediated chemo- radiotherapy among such modalities. Here, we use a cloud-based, real live cell imager an...
Abstract Background: Excess mortality serves as a valuable metric for evaluating the comprehensive health impact of healthcare systems worldwide during COVID-19 pandemic, particularly affecting vulnerable populations, including cancer patients. This study aims to estimate excess attributable all causes and cause-specific mortality, cancer, pandemic in South Korea. Methods: Monthly data from January 2000 December 2022 was obtained Statistics Using World Health Organization International...
Abstract The ability to translate a single genome into multiple phenotypes, or developmental plasticity, defines how phenotype derives from more than just genes. However, study the evolutionary targets of plasticity and their fates, we need understand genetic regulators control downstream gene expression. Here, have identified transcriptional response specific polyphenism (i.e., discrete plasticity) in nematode Pristionchus pacificus. This species produces alternative resource-use...
Abstract 27-Hydroxycholesterol (27HC) is an abundant cholesterol metabolite and has detrimental effects on the cardiovascular system, whereas its impact adiposity not well known. In this study, we found that elevations in 27HC cause increased body weight gain mice fed a high-fat/high-cholesterol diet estrogen receptor α–dependent manner. Regardless of type, fat mass was by without changes food intake or absorption. did alter energy expenditure normal chow visceral white adipose inducing...
The excretion and reabsorption of uric acid both to from urine are tightly regulated by transporters. Metabolic syndrome conditions, such as obesity, hypercholesterolemia, insulin resistance, believed regulate the expression transporters decrease acid. However, mechanisms driving cholesterol impacts on have been unknown. Here, we show that metabolite 27-hydroxycholesterol (27HC) upregulates transporter URAT1 encoded SLC22A12 via estrogen receptors (ER). Transcriptional motif analysis showed...
In land plants, two distinct generations, gametophyte and sporophyte, alternate to complete the life cycle. Sporophytes undergo meiosis produce spores, from which gametophytes develop. Gametophytes gametes, participate in fertilization zygote, first cell of sporophyte generation. addition this sexual reproduction pathway, some fern species can apospory or apogamy, processes that bypass fertilization, respectively, between generations without changing chromosome number. Apospory is inducible...
Abstract Background Despite its importance in health and disease, the cellular diversity of lung endothelium has not been systematically characterized humans. Here we provide a reference atlas human endothelial cells (ECs), to facilitate better understanding phenotypic composition comprising endothelium, both disease. Methods We reprocessed control single cell RNA sequencing (scRNAseq) data from five datasets whole lungs that were used for analysis pan-endothelial markers, later included...
Abstract Appendiceal neoplasms include a heterogeneous group of epithelial and non-epithelial tumors with varying malignant potential. Despite the rise in incidence appendiceal recent years, limited progress has been made understanding, management therapeutic treatment. To comprehensively characterize cell types molecular mechanisms driving cellular remodeling neoplasms, we performed an integrated scRNA-seq study. We analyzed 126,998 cells from 16 appendix samples (11 peritoneal metastases...
Abstract Multiple Myeloma (MM) is a hematological malignancy associated with poor prognosis and relapses. MM has strong inherited component, while the mutational transcriptomic landscapes of have been characterized extensively, molecular consequences germline genetic variation in not previously explored. Here, we leveraged matched genotype expression data from 607 patients to map somatic regulatory MM. We find that non-coding shapes transcriptional landscape MM, contributing overall...