A5011565179- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Congenital Diaphragmatic Hernia Studies
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Renal and related cancers
- Information and Cyber Security
- Tuberous Sclerosis Complex Research
- Cancer Genomics and Diagnostics
- Experimental Learning in Engineering
- Network Security and Intrusion Detection
- Advanced Malware Detection Techniques
- FOXO transcription factor regulation
- Teaching and Learning Programming
- Epigenetics and DNA Methylation
- Eosinophilic Disorders and Syndromes
- Mobile Learning in Education
- Congenital Heart Disease Studies
- Cardiac Valve Diseases and Treatments
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Peer-to-Peer Network Technologies
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
Cincinnati Children's Hospital Medical Center
2016-2025
Xiamen University
2025
University of Cincinnati
2014-2024
University of Cincinnati Medical Center
2020-2024
Perinatal Institute
2019-2023
Washington University in St. Louis
2020-2023
Oncology Hematology Care
2023
Stanford Medicine
2022
Pulmonary Associates
2019-2021
Children's Hospital of Los Angeles
2020
Idiopathic pulmonary fibrosis (IPF) is a lethal interstitial lung disease characterized by airway remodeling, inflammation, alveolar destruction, and fibrosis. We utilized single-cell RNA sequencing (scRNA-seq) to identify epithelial cell types associated biological processes involved in the pathogenesis of IPF. Transcriptomic analysis normal human cells defined gene expression patterns with highly differentiated type 2 (AT2) cells, indicated enrichment RNAs critical for surfactant...
Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations individual the variability present in population. Here we integrated Human Lung Cell Atlas (HLCA), combining 49 respiratory system into single atlas spanning over 2.4 million cells from 486 individuals. The HLCA presents consensus re-annotation with...
A major challenge in developmental biology is to understand the genetic and cellular processes/programs driving organ formation differentiation of diverse cell types that comprise embryo. While recent studies using single transcriptome analysis illustrate power measure heterogeneity complex biological systems, processing large amounts RNA-seq data from heterogeneous populations creates need for readily accessible tools single-cell (scRNA-seq) profiles. The present study presents a generally...
ABSTRACT The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, creates an urgent need for identifying molecular mechanisms that mediate viral entry, propagation, and tissue pathology. Cell membrane bound angiotensin-converting enzyme 2 (ACE2) associated proteases, transmembrane protease serine (TMPRSS2) Cathepsin L (CTSL), were previously identified as mediators of SARS-CoV2 cellular entry. Here, we assess cell type-specific RNA expression ACE2 , TMPRSS2 CTSL through integrated...
The respiratory system undergoes a diversity of structural, biochemical, and functional changes necessary for adaptation to air breathing at birth. To identify the heterogeneity pulmonary cell types dynamic in gene expression mediating respiration, here we perform single RNA analyses mouse lung on postnatal day 1. Using an iterative type identification strategy unbiasedly murine types. We distinct populations epithelial, endothelial, mesenchymal, immune cells, each containing subpopulations....
A complex organ contains a variety of cell types, each with its own distinct lineage and function. Understanding the differentiation state is fundamentally important for ultimate delineation formation We developed SLICE, novel algorithm that utilizes single-cell RNA-seq (scRNA-seq) to quantitatively measure cellular states based on single entropy predict lineages via construction directed trajectories. validated our approach using three independent data sets known developmental time...
Respiratory failure associated with COVID-19 has placed focus on the lungs. Here, we present single-nucleus accessible chromatin profiles of 90,980 nuclei and matched transcriptomes 46,500 in non-diseased lungs from donors ~30 weeks gestation,~3 years years. We mapped candidate
The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing genetic lineage tracing have revealed novel cell types enriched functional properties of existing lung. time has come to take a new census. Initiated by members the NHLBI-funded LungMAP Consortium aided experts biology community, we synthesized current data into comprehensive practical cellular census Identities normal are captured...
ABSTRACT Organ- and body-scale cell atlases have the potential to transform our understanding of human biology. To capture variability present in population, these must include diverse demographics such as age ethnicity from both healthy diseased individuals. The growth size number single-cell datasets, combined with recent advances computational techniques, for first time makes it possible generate comprehensive large-scale through integration multiple datasets. Here, we integrated Human...
Abstract Accurate cell type identification is a key and rate-limiting step in single-cell data analysis. Single-cell references with comprehensive types, reproducible functionally validated identities, common nomenclatures are much needed by the research community for automated annotation, integration, sharing. Here, we develop computational pipeline utilizing LungMAP CellCards as dictionary to consolidate transcriptomic datasets of 104 human lungs 17 mouse lung samples construct reference...
Adult mammalian cardiomyocytes (CMs) have the potential to proliferate, but this is not sufficient generate adequate CMs after myocardial infarction (MI). The transcription factor Tbx20 required for CM proliferation during development and adult homeostasis. ability of overexpression (Tbx20(OE)) promote improve cardiac function MI was examined.Tbx20(OE) induced specifically in mouse differentiated CMs. Increased fetal-like characteristics were found Tbx20(OE) hearts compared with controls...
‘LungGENS’, our previously developed web tool for mapping single-cell gene expression in the developing lung, has been well received by pulmonary research community. With continued support from ‘LungMAP’ consortium, we extended scope of LungGENS database to accommodate transcriptomics data tissues and cells human mouse at different stages lung development. Lung Gene Expression Analysis (LGEA) portal is an version useful analysis, display interpretation patterns obtained single cells, sorted...
Research Article2 March 2017Open Access Source DataTransparent process Gene signature driving invasive mucinous adenocarcinoma of the lung Minzhe Guo Perinatal Institute, Divisions Neonatology, and Pulmonary Biology, Cincinnati Children's Hospital Medical Center University College Medicine, Cincinnati, OH, USA Department Electrical Engineering Computing Systems, Search for more papers by this author Koichi Tomoshige Michael Meister Translational Unit, Thoraxklinik at Heidelberg, Lung...
Rationale: Lymphangioleiomyomatosis (LAM) is a metastatic neoplasm of reproductive-age women associated with mutations in tuberous sclerosis complex genes. LAM causes cystic remodeling the lung and progressive respiratory failure. The sources cellular characteristics cells underlying disease pathogenesis remain elusive.Objectives: Identification characterization human uterus using single-cell approach.Methods: Single-cell single-nuclei RNA sequencing on (n = 4) control 7) lungs,...
Despite high expectations for lung tumoroids, they have not been applied in the clinic due to difficulty of their long-term culture. Here, however, using AO (airway organoid) media developed by Clevers laboratory, we succeeded generating 3 tumoroid lines culture (>13 months) from 41 cancer cases (primary or metastatic). Use nutlin-3a was key selecting tumoroids that harbor mutant p53 order eliminate normal epithelial organoids. Next-generation sequencing (NGS) analysis indicated each carried BRAF
Software-defined Network (SDN) is a new architectural framework for networking that decouples control plane from data and provides programmatic interface to network control. SDN enables innovations of technologies Internet centers today in order ease the management highly dynamic complex infrastructure, support fine-grained traffic engineering, better address mobility virtual machines, services, end-points. However, itself opens many unanswered questions regarding reliability, performance,...
Disruption of alveologenesis is associated with severe pediatric lung disorders, including bronchopulmonary dysplasia (BPD). Although c-KIT
Although modern clinical practices such as cesarean sections and perinatal antibiotics have improved infant survival, treatment with broad-spectrum alters intestinal microbiota causes dysbiosis. Infants exposed to an increased likelihood of life-threatening infections, including pneumonia. Here, we investigated how the gut sculpt pulmonary immune responses, promoting recovery resolution infection in newborn rhesus macaques. Early-life antibiotic exposure interrupted maturation commensal...
In order to reach the goals of Information Security Automation Program (ISAP) [1], we propose an ontological approach capturing and utilizing fundamental concepts in information security their relationship, retrieving vulnerability data reasoning about cause impact vulnerabilities. Our ontology for management (OVM) has been populated with all vulnerabilities NVD [2] additional inference rules, knowledge representation, data-mining mechanisms. With seamless integration common related such as...