A5020992768- Glaucoma and retinal disorders
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Circadian rhythm and melatonin
- Neonatal Respiratory Health Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Retinal Imaging and Analysis
- Calpain Protease Function and Regulation
- Single-cell and spatial transcriptomics
- Retinopathy of Prematurity Studies
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Retinal and Optic Conditions
- Extracellular vesicles in disease
- Cell Image Analysis Techniques
- Biofield Effects and Biophysics
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neural Engineering
- Ophthalmology and Eye Disorders
- Zebrafish Biomedical Research Applications
- Ocular Diseases and Behçet’s Syndrome
Galapagos (Belgium)
2023
VIB-KU Leuven Center for Brain & Disease Research
2019-2022
KU Leuven
2015-2019
Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with single-cell RNA sequencing induced multilineage circuit fibrogenic cell states hPCLS. We showed that these were highly similar to multicohort atlas from patients pulmonary...
According to the neurotrophin deprivation hypothesis, diminished retrograde delivery of neurotrophic support during an early stage glaucoma pathogenesis is one main triggers that induce retinal ganglion cell (RGC) degeneration. Therefore, interfering with signaling seems attractive strategy achieve neuroprotection. Indeed, exogenous administration eye has been shown reduce loss RGCs in animal models glaucoma; however, neuroprotective effect was mostly insufficient for sustained RGC survival....
Homeostatic turnover of the trabecular meshwork extracellular matrix (ECM) is essential to regulate aqueous humor outflow and maintain intraocular pressure homeostasis. In this study, we evaluated turnover, pressure, organization in MMP-9 null mice.Intraocular were measured versus wild-type mice. Morphology anterior segment eye, with special attention structural meshwork, was investigated by means optical coherence tomography, light microscopy, transmission electron microscopy. Furthermore,...
Glaucoma is characterized by a progressive loss of retinal ganglion cells (RGCs) in the eye, which ultimately results visual impairment or even blindness. Because current therapies often fail to halt disease progression, there an unmet need for novel neuroprotective support RGC survival. Various research lines suggest that target centers brain functioning and Here, we explored whether increasing neuronal activity one these projection areas could improve survival RGCs mouse glaucoma model....
ABSTRACT Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with scRNAseq induced multi-lineage circuit fibrogenic cell states hPCLS, which show to be highly similar multi-cohort atlas from pulmonary patients. Using micro-CT staged...
One important facet of glaucoma pathophysiology is axonal damage, which ultimately disrupts the connection between retina and its postsynaptic brain targets. The concurrent loss retrograde support interferes with functionality survival retinal ganglion cells (RGCs). Previous research has shown that stimulation neuronal activity in a primary target area—i.e., superior colliculus—promotes RGC an acute mouse model glaucoma. To build further on this observation, we applied repeated chemogenetics...
In the August issue of Nature Neuroscience , Lim and co-workers reported on their study axonal regeneration in adult optic nerve, revealing that combined activation mammalian target rapamycin (mTOR) signaling enhancing neural activity leads to successful restoration trajectories visual brain centers (1). Here, we shed light breakthrough findings how these may represent a major leap forward search for novel strategies central nervous system (CNS) repair.
Our overall objective is to investigate whether modulation of neuronal activity in the superior colliculus (SC), principal retinal target structure and main extraretinal neurotrophic factor source mice, can protect against chronic RGC loss, experimentally induced using well-establised optic nerve crush (ONC) mouse model glaucoma. For modulation, we will make use novel revolutionary technology optogenetics. More precisely, this project aim to: 1) validate genetic labeling SC neurons with AAV...
Purpose Aqueous humor outflow resistance depends on a complex equilibrium of extracellular matrix biosynthesis versus proteolysis in the trabecular meshwork. Several members MMP family, including MMP-9, are essential to regulation aqueous resistance, i.e. intraocular pressure (IOP) homeostasis. Here, we characterized baseline ocular phenotype MMP-9 null mice, with emphasis dysregulation IOP Methods and wild type from 3 13 months age, were studied. central corneal thickness measured via...