A5057394108- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Transplantation: Methods and Outcomes
- Sarcoidosis and Beryllium Toxicity Research
- Medical Imaging and Pathology Studies
- Systemic Sclerosis and Related Diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pulmonary Hypertension Research and Treatments
- Occupational and environmental lung diseases
- Organ Transplantation Techniques and Outcomes
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Respiratory viral infections research
- Inflammatory Myopathies and Dermatomyositis
- Renal Transplantation Outcomes and Treatments
- Occupational exposure and asthma
- Voice and Speech Disorders
- Radiomics and Machine Learning in Medical Imaging
- Ultrasound in Clinical Applications
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Polyomavirus and related diseases
- Eosinophilic Disorders and Syndromes
- Advanced X-ray and CT Imaging
- Mechanical Circulatory Support Devices
KU Leuven
2017-2025
German Center for Lung Research
2023-2024
Helmholtz Zentrum München
2023-2024
Ludwig-Maximilians-Universität München
2023
Universitair Ziekenhuis Leuven
2018-2022
Ziekenhuis Oost-Limburg
2021
Hôpital Louis Pradel
2019
University of Padua
2019
Royal Brompton Hospital
2019
To develop a systems biology model of fibrosis progression within the human lung we performed RNA sequencing and microRNA analysis on 95 samples obtained from 10 idiopathic pulmonary (IPF) 6 control lungs. Extent in each sample was assessed by microCT-measured alveolar surface density (ASD) confirmed histology. Regulatory gene expression networks were identified using linear mixed-effect models dynamic regulatory events miner (DREM). Differential core set genes increased or decreased before...
Fibroblast-to-myofibroblast conversion is a major driver of tissue remodelling in organ fibrosis. Distinct lineages fibroblasts support homeostatic niche functions, yet their specific activation states and phenotypic trajectories during injury repair have remained unclear.
Background: Although the third most frequent interstitial lung disease, hypersensitivity pneumonitis (HP) remains an enigmatic disease without clear diagnostic and therapeutic guidelines. We assessed effect of commonly used interventions (i.e. exposure avoidance corticosteroid treatment) in HP cohort. Methods: collected clinical data all patients followed at our centre between January 1, 2005, December 31, 2016. were stratified according to presence fibrosis on chest CT. Survival was...
Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms and fibrogenesis directly in human tissue has been hampered by the lack organotypic models analytical techniques. In this work, we coupled ex vivo cytokine drug perturbations precision-cut lung slices (hPCLS) with single-cell RNA sequencing induced multilineage circuit fibrogenic cell states hPCLS. We showed that these were highly similar to multicohort atlas from patients pulmonary...
Telomere shortening has been associated with several lung diseases. However, telomere length is generally measured in peripheral blood leucocytes rather than tissue, where disease occurs. Consequently, dynamics have not established for the normal human nor diseased tissue. We hypothesized an age- and disease-dependent of tissue telomeres. At time (re-)transplantation or autopsy, 70 explant lungs were collected: from unused donors (normal, n = 13) patients cystic fibrosis (CF, 12), chronic...
Rationale: Fibrotic hypersensitivity pneumonitis (fHP) is an interstitial lung disease caused by sensitization to inhaled allergen. Objectives: To identify the molecular determinants associated with progression of fibrosis. Methods: Nine fHP explant lungs and six unused donor (as controls) were systematically sampled (4 samples/lung). According microcomputed tomography measures, cores clustered into mild, moderate, severe fibrosis groups. Gene expression profiles assessed using weighted gene...
In this research, we delve into the association between epigenetic aging and idiopathic pulmonary fibrosis (IPF), a debilitating lung disease that progresses over time. Utilizing Illumina MethylationEPIC array, assessed DNA methylation levels in donated human tissue from IPF patients, categorizing mild, moderate, severe stages based on clinical assessments. We employed seven clocks to determine age acceleration, which is discrepancy biological (epigenetic) chronological age. Our findings...
Objectives Interstitial lung disease associated with rheumatoid arthritis (RA-ILD) is linked to high mortality. Currently, effective screening tools are lacking. We assessed the role of symptoms and ultrasound (LUS) as potential tools. Methods 116 adult patients RA presenting rheumatology outpatient clinic underwent high-resolution CT (HRCT) scans, pulmonary function tests, LUS (72 zones) completed a Visual Analogue Scale (VAS) for cough modified Medical Research Council dyspnoea scale...
Spatial heterogeneity in disease patterns is a key hallmark of interstitial lung diseases (ILDs), such as systemic sclerosis (SSc)-ILD. High-dimensional image analysis (radiomics) computed tomography (CT) scans offers quantitative insights into organ-scale pathophysiology, creating digital fingerprints. Here, we aimed to integrate spatially resolved radiomic profiles extracted from CT with matched molecular data decipher the cellular programs underlying spatial ILDs and how they are...
Introduction Compared with traditional static ice storage, controlled hypothermic storage (CHS) at 4–10°C may attenuate cold-induced lung injury between procurement and implantation. In this study, we describe the first European transplant (LTx) experience a portable CHS device. Methods A prospective observational study was conducted of all consecutively performed LTx following (11 November 2022 31 January 2024) two high-volume centers. The LUNGguard device used for CHS. preservation...
COPD is characterized by chronic airway inflammation, small airways changes, with disappearance and obstruction, also distal/alveolar destruction (emphysema). The chronology which these three features evolve altered mucosal immunity remains elusive. This study assessed the immune defense in human control end-stage lungs, detailed microCT RNA transcriptomic analysis of diversely affected zones.In 11 (non-used donors) (end-stage) explant frozen 4 cylinders/cores were processed per lung for...
Idiopathic pulmonary fibrosis (IPF) with coexistent emphysema, termed combined and emphysema (CPFE) may associate reduced forced vital capacity (FVC) declines compared to non-CPFE IPF patients. We examined associations between mortality functional measures of disease progression in two cohorts.
Abstract Background and objective Although idiopathic pulmonary fibrosis (IPF) patients experience a worse survival compared with chronic hypersensitivity pneumonitis (CHP), organic dust exposure is known risk factor for both IPF CHP. Methods We divided diagnosed IPF, based on their to moulds/birds (absent: group A; present: B). retrospectively function between groups A B, separate CHP cohort (group C). Results total of 293 were included A: n = 171, B: 73, C: 49). Demographics baseline did...