A5082667243- Transplantation: Methods and Outcomes
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Mechanical Circulatory Support Devices
- Immune Response and Inflammation
- Polyomavirus and related diseases
- Respiratory viral infections research
- Antifungal resistance and susceptibility
- Immune Cell Function and Interaction
- Immune cells in cancer
- Connective Tissue Growth Factor Research
- Respiratory Support and Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Medical Imaging and Pathology Studies
- Thermal Regulation in Medicine
- RNA Interference and Gene Delivery
- Telomeres, Telomerase, and Senescence
- Orthopaedic implants and arthroplasty
- Extracellular vesicles in disease
- COVID-19 and healthcare impacts
- T-cell and B-cell Immunology
KU Leuven
2016-2024
Universitair Ziekenhuis Leuven
2017-2024
VIB-KU Leuven Center for Microbiology
2017
Rationale: Fibrotic hypersensitivity pneumonitis (fHP) is an interstitial lung disease caused by sensitization to inhaled allergen. Objectives: To identify the molecular determinants associated with progression of fibrosis. Methods: Nine fHP explant lungs and six unused donor (as controls) were systematically sampled (4 samples/lung). According microcomputed tomography measures, cores clustered into mild, moderate, severe fibrosis groups. Gene expression profiles assessed using weighted gene...
Pirfenidone may attenuate the decline of pulmonary function in restrictive allograft syndrome (RAS) after lung transplantation. We retrospectively assessed all transplant recipients with RAS who were treated pirfenidone for at least 3 months (n = 11) our center and report on their long-term outcomes following initiation pirfenidone. Main outcome parameters included evolution overall survival. appears to forced vital capacity expiratory volume 1 second. Notably, patients bridged...
Rationale Recent studies suggest that similar injury mechanisms are in place across different solid organ transplants, resulting the identification of a common rejection module (CRM), consisting 11 genes overexpressed during acute and, to lesser extent, chronic allograft rejection. Objectives We wanted evaluate usefulness CRM identifying (AR) and phenotypes lung transplant (CLAD), i.e., bronchiolitis obliterans syndrome (BOS) restrictive (RAS), using transbronchial brushings,...
Introduction Circulating anti-human leukocyte antigen (HLA) serum donor-specific antibodies (sDSAs) increase the risk of chronic lung allograft dysfunction (CLAD) and mortality. Discrepancies between serological pathological/clinical findings are common. Therefore, we aimed to assess presence tissue-bound graft DSAs (gDSAs) in CLAD explant tissue compared with sDSAs. Methods Tissue cores, obtained from lungs unused donors (n=10) patients bronchiolitis obliterans syndrome (BOS; n=18)...
Long-term survival after lung transplantation (LTx) is hampered by development of chronic allograft dysfunction (CLAD). Pseudomonas aeruginosa an established risk factor for CLAD. Therefore, we investigated the effect P. eradication on CLAD-free and graft survival. Patients who underwent first LTx between July, 1991, February, 2016, were free from CLAD, retrospectively classified according to presence in respiratory samples September, 2011, 2016. -positive patients subsequently stratified...
Eosinophils play a role in many chronic lung diseases. In transplantation (LTx), increased eosinophils bronchoalveolar lavage (BAL) was associated with worse outcomes. However, the effect of peripheral blood eosinophilia after LTx has not been investigated thoroughly. A retrospective study performed including all patients between 2011-2016. Chronic allograft dysfunction (CLAD)-free and graft survival were compared high low using an 8% threshold ever during follow-up. total 102 (27.1%) had...
Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of immune from the myeloid lineage. MDSCs expand in pathological situations, such as chronic infection, cancer, autoimmunity and allograft rejection. As lung dysfunction (CLAD) limits long-term survival after transplantation (LTx), may play role its pathophysiology. We assessed phenotype frequency peripheral blood transplant recipients relationship to post-transplant complications immunosuppression. Granulocytic (G)-MDSC were...
Despite donor organ shortage, a large proportion of possible lungs are declined for transplantation. Criteria accepting/declining remain controversial because the lack adequate tools to aid in decision-making. We collected, air-inflated, and froze series declined/unused subjected these lung specimens CT examination. Affected target regions were scanned by using micro-CT. Lungs from 28 donors collected. Two unused, six non-allograft-related reasons (collectively denominated nonallograft...
In chronic obstructive pulmonary disease (COPD), the bronchial epithelium is first immune barrier that triggered by cigarette smoke. Although vitamin D (vitD) has proven anti-inflammatory and antimicrobial effects in alveolar macrophages, little known about direct role of vitD on smoke-exposed epithelial cells. We examined a human cell line (16HBE) air–liquid culture primary cells (PBEC) COPD patients controls exposed for 24 h to smoke extract (CSE). VitD decreased CSE-induced IL-8 secretion...
Background Connective tissue growth factor (CTGF) is an important mediator in several fibrotic diseases, including lung fibrosis. We investigated CTGF-expression chronic allograft dysfunction (CLAD) and pulmonary graft-versus-host disease (GVHD). Materials Methods CTGF expression was assessed by quantitative real-time polymerase chain reaction (qPCR) immunohistochemistry end-stage CLAD explant (bronchiolitis obliterans syndrome (BOS), n=20; restrictive (RAS), n=20), GHVD (n=9). Unused donor...
Background. Bronchiolitis obliterans syndrome (BOS) after lung transplantation is characterized by fibrotic small airway remodeling, recognizable on high-resolution computed tomography (HRCT). We studied the prognostic value of key HRCT features at BOS diagnosis transplantation. Methods. The presence and severity bronchiectasis, mucous plugging, peribronchial thickening, parenchymal anomalies, air trapping, summarized in a total score, were assessed using simplified Brody II scoring system...
Ex vivo lung perfusion (EVLP) is a widespread accepted platform for preservation and evaluation of donor lungs prior to transplantation (LTx). Standard are ventilated using volume-controlled ventilation (VCV). We investigated the effects flow-controlled (FCV) in large animal EVLP model. Fourteen porcine were mounted on after warm ischemic interval 2 h randomized two groups (n = 7/group). In VCV, 7 grafts conventionally FCV, by ventilation. physiologic parameters (compliance, pulmonary...
Primary graft dysfunction (PGD) remains a major post-transplant complication and is associated with increased morbidity mortality. Mechanisms evoking PGD are not completely clear, but inflammation plays central role. We investigated the association between inflammatory proteins present in immediate postoperative bronchoalveolar lavage.All double-lung recipients transplanted at our institution from 2002 to 2018 were included study. retrospectively selected 80 consecutive lung transplant...
Fungal exposure and sensitization negatively affect outcomes in various respiratory diseases, however, the effect of fungal lung transplant (LTx) recipients is still unknown. We performed a retrospective cohort study prospectively collected data on circulating specific IgG/IgE antibodies, their correlation with isolation, chronic allograft dysfunction (CLAD) overall survival after LTx. 311 patients transplanted between 2014 2019 were included. Patients elevated Aspergillus fumigatus or...