A5069396153- Connective Tissue Growth Factor Research
- Chronic Kidney Disease and Diabetes
- Systemic Sclerosis and Related Diseases
- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Chronic Myeloid Leukemia Treatments
- AI in cancer detection
- Protein Degradation and Inhibitors
- Radiomics and Machine Learning in Medical Imaging
- Renal and Vascular Pathologies
- Chronic Lymphocytic Leukemia Research
- Biomarkers in Disease Mechanisms
- Cardiac Arrhythmias and Treatments
- Dialysis and Renal Disease Management
- Genetic and Kidney Cyst Diseases
- Connective tissue disorders research
- Artificial Intelligence in Healthcare and Education
- Cardiac electrophysiology and arrhythmias
- Parathyroid Disorders and Treatments
- Atrial Fibrillation Management and Outcomes
- TGF-β signaling in diseases
- Cardiac pacing and defibrillation studies
- MRI in cancer diagnosis
- Birth, Development, and Health
- Nuclear reactor physics and engineering
University Medical Center Utrecht
2016-2025
Heidelberg University
2010-2025
University Hospital Heidelberg
2010-2025
Abbott (Canada)
2025
Pennsylvania State University
2022-2024
Erasmus MC
2023
University Medical Center Groningen
2023
University of Groningen
2023
Wilhelmina Children's Hospital
2023
Leiden University Medical Center
2023
The reversibility of diabetic nephropathy remains controversial. Here, we tested whether replacing leptin could reverse the advanced modeled by leptin-deficient BTBR ob/ob mouse. Leptin replacement, but not inhibition renin-angiotensin-aldosterone system (RAAS), resulted in near-complete reversal both structural (mesangial matrix expansion, mesangiolysis, basement membrane thickening, podocyte loss) and functional (proteinuria, accumulation reactive oxygen species) measures nephropathy....
Large studies on long-term kidney outcome in patients with anti-glomerular basement membrane (anti-GBM) GN are lacking. This study aimed to identify clinical and histopathologic parameters that predict these patients.This retrospective analysis included a total of 123 anti-GBM between 1986 2015 from six centers worldwide. Their biopsy samples were classified according the classification for ANCA-associated GN. Clinical data such as details treatment retrieved records. The primary parameter...
BackgroundHistopathological assessment of transplant biopsies is currently the standard method to diagnose allograft rejection and can help guide patient management, but it one most challenging areas pathology, requiring considerable expertise, time, effort. We aimed analyse utility deep learning preclassify histology kidney into three main broad categories (ie, normal, rejection, other diseases) as a potential biopsy triage system focusing on rejection.MethodsWe performed retrospective,...
The rapid introduction of digital pathology has greatly facilitated development artificial intelligence (AI) models in that have shown great promise assisting morphological diagnostics and quantitation therapeutic targets. We are now at a tipping point where companies started to bring algorithms the market, questions arise whether community is ready implement AI routine workflow. However, concerns also about use pathology. This article reviews pros cons introducing diagnostic
In diabetic nephropathy, connective tissue growth factor (CTGF) is upregulated and bone morphogenetic protein 7 (BMP-7) downregulated. CTGF known to inhibit BMP-4, but similar cross-talk between BMP-7 has not been studied. this study, it was hypothesized that acts as an inhibitor of signaling activity in nephropathy. Compared with wild-type CTGF(+/+) mice, CTGF(+/-) mice had approximately 50% lower mRNA protein, less severe albuminuria, no thickening the glomerular basement membrane,...
We evaluated the predictive value of baseline plasma connective tissue growth factor (CTGF) in a prospective study patients with type 1 diabetes.Subjects were 198 diabetic established nephropathy and 188 persistent normoalbuminuria. Follow-up time was 12.8 years. Prediction end-stage renal disease (ESRD) mortality by CTGF analyzed conjunction conventional risk factors.Plasma higher than normoalbuminuria (median 381 [interquartile range 270-630] vs. 235 [168-353] pmol/l). In nephropathy,...
Arrhythmogenic ventricular remodeling is hallmarked by both reduced gap junction expression and increased collagen deposition. We hypothesized that connexin43 (Cx43) responsible for enhanced fibrosis in the remodeled heart, resulting an arrhythmogenic substrate. Therefore, we investigated effect of normal or Cx43 on formation a physiological (aging) pathophysiological (transverse aortic constriction [TAC]) mouse model.The Cx43(fl/fl) Cx43(CreER(T)/fl) mice were aged 18 to 21 months or, at...
We recently reported that centrosomal protein 164 (CEP164) regulates both cilia and the DNA damage response in autosomal recessive polycystic kidney disease nephronophthisis. Here we examine functional role of CEP164 nephronophthisis-related ciliopathies concomitant fibrosis. Live cell imaging RPE-FUCCI (fluorescent, ubiquitination-based cycle indicator) cells after siRNA knockdown revealed an overall quicker than control cells, although early S-phase was significantly longer. Follow-up FACS...
Obesity-induced inflammation presumably accelerates the development of chronic kidney diseases. However, little is known about sequence these inflammatory events and their contribution to renal pathology. We investigated effects obesity on evolution age-dependent complications in mice conjunction with systemic low-grade (LGI). C57BL/6J susceptible develop sclerotic pathologies amyloid features kidney, were fed low (10% lard) or high-fat diets (45% for 24, 40 52 weeks. HFD-feeding induced...
Abstract Pathologists’ assessment of sentinel lymph nodes (SNs) for breast cancer (BC) metastases is a treatment-guiding yet labor-intensive and costly task because the performance immunohistochemistry (IHC) in morphologically negative cases. This non-randomized, single-center clinical trial (International Standard Randomized Controlled Trial Number:14323711) assessed efficacy an artificial intelligence (AI)-assisted workflow detecting BC SNs while maintaining diagnostic safety standards....
ABSTRACT Septins have been shown to play important roles in cytokinesis diverse organisms ranging from yeast mammals. In this study, we show that both the unc-59 and unc-61 loci encode Caenorhabditis elegans septins. Genomic database searches indicate are probably only septin genes C. genome. UNC-59 UNC-61 localize leading edge of cleavage furrows eventually reside at midbody. Analysis mutants revealed each requires presence other for localization cytokinetic furrow. Surprisingly, generally...
OBJECTIVE—Levels of connective tissue growth factor (CTGF; CCN-2) in plasma are increased various fibrotic disorders, including diabetic nephropathy. Recently, several articles have reported a strong increase urinary CTGF excretion (U-CTGF) patients with However, these studies addressed too small number to allow general conclusions be drawn. Therefore, we evaluated U-CTGF large cross-sectional study type 1 diabetes. RESEARCH DESIGN AND METHODS—Subjects were 318 and 29 normoglycemic control...
OBJECTIVE—Levels of connective tissue growth factor (CTGF; CCN-2) in plasma are increased various fibrotic disorders, including diabetic nephropathy. Recently, several articles have reported a strong increase urinary CTGF excretion (U-CTGF) patients with However, these studies addressed too small number to allow general conclusions be drawn. Therefore, we evaluated U-CTGF large cross-sectional study type 1 diabetes.