A5044176362- Connective Tissue Growth Factor Research
- Cardiovascular Function and Risk Factors
- Peroxisome Proliferator-Activated Receptors
- Cardiac Fibrosis and Remodeling
- Connective tissue disorders research
- Metabolism and Genetic Disorders
- Systemic Sclerosis and Related Diseases
- Adipose Tissue and Metabolism
- Cardiac Valve Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- Cardiac Ischemia and Reperfusion
- Ion channel regulation and function
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Atrial Fibrillation Management and Outcomes
- TGF-β signaling in diseases
- Cardiac Structural Anomalies and Repair
- Viral Infections and Immunology Research
- Cardiac electrophysiology and arrhythmias
- Fuel Cells and Related Materials
- Cardiovascular Disease and Adiposity
- Erythrocyte Function and Pathophysiology
- Cardiovascular Effects of Exercise
- Heat shock proteins research
Maastricht University
2013-2025
University Medical Center
2021-2022
Universidad de Navarra
2017
Navarre Institute of Health Research
2017
Instituto de Salud Carlos III
2017
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017
Leiden University Medical Center
2017
Netherlands Heart Institute
2017
VIB-KU Leuven Center for Cancer Biology
2017
University Medical Center Utrecht
2003-2008
BackgroundIn proliferative diabetic retinopathy (PDR), vascular endothelial growth factor (VEGF) and connective tissue (CTGF) cause blindness by neovascularization subsequent fibrosis, but their relative contribution to both processes is unknown. We hypothesize that the balance between levels of pro-angiogenic VEGF pro-fibrotic CTGF regulates angiogenesis, angio-fibrotic switch, resulting fibrosis scarring.Methods/Principal FindingsVEGF were measured ELISA in 68 vitreous samples patients...
Background Myoglobin and fatty acid–binding protein (FABP) each are useful as early biochemical markers of muscle injury. We studied whether the ratio myoglobin over FABP in plasma can be used to distinguish myocardial from skeletal Methods Results were assayed immunochemically tissue samples human heart serial 22 patients with acute infarction (AMI), 9 undergoing aortic surgery (causing injury muscles), 10 cardiac surgery. In tissue, myoglobin/FABP was 4.5 muscles varied 21 73. After AMI,...
Pressure overload causes cardiac fibroblast activation and transdifferentiation, leading to increased interstitial fibrosis formation subsequently myocardial stiffness, diastolic systolic dysfunction, eventually heart failure. A better understanding of the molecular mechanisms underlying pressure overload–induced remodeling will have implications for failure treatment strategies. The microRNA (miRNA)-221/222 family, consisting miR-221-3p miR-222-3p, is differentially regulated in mouse human...
Piezo1 is a mechanosensitive cation channel with widespread physiological importance; however, its role in the heart poorly understood. Cardiac fibroblasts help preserve myocardial integrity and play key regulating repair remodeling following stress or injury. Here we investigated expression function cultured human mouse cardiac fibroblasts. RT-PCR experiments confirmed that mRNA expressed at levels similar to those endothelial cells. The results of Fura-2 intracellular Ca2+ assay validated...
The precise mechanism of uptake long-chain fatty acids (FA) by cardiac myocytes is incompletely understood. We examined the involvement sarcolemmal proteins in initial FA isolated rat myocytes, and relation between metabolism. Cardiac were incubated presence 90 microns [1-14C]palmitate complexed to 300 bovine serum albumin (BSA), presenting a physiologically relevant condition. During palmitate (3 min), 56% intracellularly sequestered was esterified, an additional 21% converted into...
BACKGROUND--There are substantial amounts of cytoplasmic heart-type fatty-acid-binding protein (FABP) (15 kDa) in myocardial tissue. The rapid release FABP into plasma during ischaemia indicates the possibility using this as a biochemical marker for ischaemic injury. OBJECTIVE--To study completeness from damaged tissue patients with acute infarction (AMI) and suitability serial concentrations estimation infarct size. METHODS--Immunochemically assayed enzymatically creatine kinase isoenzyme...
In diabetic nephropathy, connective tissue growth factor (CTGF) is upregulated and bone morphogenetic protein 7 (BMP-7) downregulated. CTGF known to inhibit BMP-4, but similar cross-talk between BMP-7 has not been studied. this study, it was hypothesized that acts as an inhibitor of signaling activity in nephropathy. Compared with wild-type CTGF(+/+) mice, CTGF(+/-) mice had approximately 50% lower mRNA protein, less severe albuminuria, no thickening the glomerular basement membrane,...
AimsPeroxisome proliferator-activated receptor-α (PPARα) is a nuclear receptor regulating cardiac metabolism that also has anti-inflammatory properties. Since the activation of inflammatory signalling pathways considered to be important in hypertrophy and fibrosis, it anticipated PPARα modulates remodelling. Accordingly, this study hypothesis was tested absence aggravates hypertrophic response pressure overload.
We evaluated the predictive value of baseline plasma connective tissue growth factor (CTGF) in a prospective study patients with type 1 diabetes.Subjects were 198 diabetic established nephropathy and 188 persistent normoalbuminuria. Follow-up time was 12.8 years. Prediction end-stage renal disease (ESRD) mortality by CTGF analyzed conjunction conventional risk factors.Plasma higher than normoalbuminuria (median 381 [interquartile range 270-630] vs. 235 [168-353] pmol/l). In nephropathy,...
Tubulointerstitial fibrosis is an important component in the development of diabetic nephropathy. Various renal cell types, including fibroblasts, contribute to excessive matrix deposition kidney. Although transforming growth factor-β (TGF-β) has been thought play a major role during fibrosis, other factors are also involved. Here we examined effects connective tissue factor (CTGF) and IGF-I on collagen type I III production by human fibroblasts their involvement glucose-induced...
To generate antibodies to defined cell‐surface antigens, we used a large phage antibody fragment library select on cell transfectants expressing one of three chosen receptors. First, in vitro panning procedures and screening ELISAs were developed using whole live cells stably the antigen interest. When these methodologies applied Chinese hamster ovary (CHO) receptors for neuropeptide, somatostatin, either direct or strategy depletion ligand‐directed elution, many different pan‐CHO‐cell...
OBJECTIVE—Connective tissue growth factor (CTGF) is strongly upregulated in fibrotic disorders and has been hypothesized to play a role the development progression of diabetes complications. The aim present study was investigate possible association plasma CTGF levels type 1 diabetic patients with markers relevant RESEARCH DESIGN AND METHODS—Plasma (full-length NH2-terminal fragments) were determined 62 well-characterized 21 healthy control subjects. Correlations these glycemic control,...
OBJECTIVE—Levels of connective tissue growth factor (CTGF; CCN-2) in plasma are increased various fibrotic disorders, including diabetic nephropathy. Recently, several articles have reported a strong increase urinary CTGF excretion (U-CTGF) patients with However, these studies addressed too small number to allow general conclusions be drawn. Therefore, we evaluated U-CTGF large cross-sectional study type 1 diabetes. RESEARCH DESIGN AND METHODS—Subjects were 318 and 29 normoglycemic control...
<h3>Objective</h3> To investigate the expression of profibrotic connective tissue growth factor (CTGF) in relation to severity intraocular fibrosis and neovascularization human vitreoretinal disorders for identification potential therapeutic targets prevent fibrosis. <h3>Methods</h3> Concentrations CTGF were measured by enzyme-linked immunosorbent assay 119 vitreous samples from patients with proliferative diabetic retinopathy, vitreoretinopathy, epiretinal membrane, macular hole. Clinical...
Abstract Aim: To identify the initial alterations in myocardial tissue associated with early signs of diabetic cardiac haemodynamic dysfunction, we monitored changes function, structural remodelling and gene expression hearts type 2 db/db mice. Methods: Cardiac dimensions function were determined echocardiographically at 8, 12, 16 18 weeks age. Left ventricular pressure characteristics measured under baseline conditions upon dobutamine infusion. Results: The mice severely already 8 after...
Abstract Heart failure is accompanied by extracellular matrix (ECM) remodelling, often leading to cardiac fibrosis. In the present study we explored significance of cartilage intermediate layer protein 1 (CILP1) as a novel mediator ECM remodelling. Whole genome transcriptional analysis human tissue samples revealed strong association CILP1 with many structural (e.g. COL1A2 r 2 = 0.83) and non-structural TGFB3 0.75) proteins. Gene enrichment further underscored involvement in remodelling TGFβ...
The diabetic heart is characterized by extensive lipid accumulation which often leads to cardiac contractile dysfunction. underlying mechanism involves a pivotal role for vacuolar-type H+-ATPase (v-ATPase, functioning as endosomal/lysosomal proton pump). Specifically, oversupply the causes disassembly of v-ATPase and endosomal deacidification. Endosomes are storage compartments transporter CD36. However, upon deacidification, CD36 expelled translocate sarcolemma, thereby inducing myocardial...