A5035843711- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Cardiac Valve Diseases and Treatments
- Coronary Artery Anomalies
- Congenital Heart Disease Studies
- Cardiac Fibrosis and Remodeling
- Colorectal Cancer Treatments and Studies
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Chemotherapy-induced cardiotoxicity and mitigation
- Aortic aneurysm repair treatments
- Acute Myeloid Leukemia Research
- Multilevel Inverters and Converters
- Coronary Interventions and Diagnostics
- Circular RNAs in diseases
- Cancer Cells and Metastasis
- Cancer Treatment and Pharmacology
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Cancer, Lipids, and Metabolism
- Genetic factors in colorectal cancer
- Cardiovascular Disease and Adiposity
- Virus-based gene therapy research
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
Leiden University Medical Center
2016-2024
Oncode Institute
2018-2020
Amsterdam UMC Location University of Amsterdam
2016-2018
Universidad de Navarra
2017
Maastricht University
2017
Navarre Institute of Health Research
2017
Instituto de Salud Carlos III
2017
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017
VIB-KU Leuven Center for Cancer Biology
2017
Netherlands Heart Institute
2017
Pressure overload causes cardiac fibroblast activation and transdifferentiation, leading to increased interstitial fibrosis formation subsequently myocardial stiffness, diastolic systolic dysfunction, eventually heart failure. A better understanding of the molecular mechanisms underlying pressure overload–induced remodeling will have implications for failure treatment strategies. The microRNA (miRNA)-221/222 family, consisting miR-221-3p miR-222-3p, is differentially regulated in mouse human...
Significance Anthracyclines like doxorubicin are anticancer drugs, used by over 1 million cancer patients annually. However, they cause severe side effects, most notably, cardiotoxicity and therapy-related malignancies. It is unclear whether these effects directly linked to their activity. Doxorubicin exerts two activities: DNA damage chromatin damage. Here, we show that both activities conspire in the cardiotoxicity, while variants with only chromatin-damaging activity remain active drugs...
Abstract Background The epicardium, a cell layer covering the heart, plays an important role during cardiogenesis providing cardiovascular types and instructive signals, but becomes quiescent adulthood. Upon cardiac injury epicardium is activated, which includes induction of developmental gene program, epithelial-to-mesenchymal transition (EMT) migration. However, response adult suboptimal compared to active contribution fetal heart development. To understand therapeutic value...
Patient‐derived xenograft (PDX) models have become an important asset in translational cancer research. However, to provide a robust preclinical platform, PDXs need accommodate the tumor heterogeneity that is observed patients. Colorectal (CRC) can be stratified into four consensus molecular subtypes (CMS) with distinct biological and clinical features. Surprisingly, using set of CRC patients, we revealed partial representation PDX models. The epithelial subtypes, largest subgroups subtype,...
The epicardium, an epithelial cell layer covering the myocardium, has essential role during cardiac development, as well in repair response of heart after ischemic injury. When activated, epicardial cells undergo a process known to mesenchymal transition (EMT) provide regenerating myocardium. Furthermore, epicardium contributes via secretion paracrine factors. To fully appreciate regenerative potential human model is required. Here we outline novel culture derive primary derived (EPDCs) from...
The epicardium plays an essential role in cardiogenesis by providing cardiac cell types and paracrine cues to the developing myocardium. human adult is quiescent, but recapitulation of developmental features may contribute repair. fate epicardial cells proposed be determined persistence specific subpopulations. Reports on this heterogeneity have been inconsistent, data regarding are scarce. Here we specifically isolated fetal used single-cell RNA sequencing define its composition identify...
The data and information presented here refer to the research article entitled: "Reactivating endogenous mechanisms of cardiac regeneration via paracrine boosting with human amniotic fluid stem cell secretome" (Balbi et al., 2019, Apr 04). This dataset illustrates in vitro effect exerted by secretome on rodent neonatal cardiomyocytes, endothelial progenitors different subsets progenitor cells. Cytokine/chemokine profiling is provided as well. can provide useful insights regenerative medicine...
ABSTRACT Patients with a congenital bicuspid aortic valve (BAV), two instead of three leaflets, have an increased risk developing thoracic aneurysms and dissection. The mechanisms underlying BAV-associated aortopathy are poorly understood. This study examined in Nos3−/− mice, model BAV formation. A combination histological examination vivo ultrasound imaging was used to investigate dilation dissections mice. Moreover, cell lineage analysis single-cell RNA sequencing were observe the...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Dutch Heart Foundation During development, the epicardium forms as a single cell layer on outside heart. Part these cells undergo epithelial-to-mesenchymal transition (EMT) and migrate into myocardium where epicardial-derived (EPDCs) participate in formation heart by contributing producing paracrine factors. In adult exists dormant until there is ischemic injury which leads to partial reactivation...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Dutch Heart Foundation Background The outside mesothelial layer the heart, epicardium, is essential for cardiac development. Cells from this undergo epithelial-to-mesenchymal transition (EMT), migrate into tissue and partake in cardiogenesis by contributing cells secreting growth factors. In adult epicardium quiescent but upon ischemic injury it activated conveys a partial regenerative response. This...
The epicardium, the mesothelial layer covering heart, is a crucial cell source for cardiac development and repair. It provides cells biochemical signals to heart facilitate vascularization myocardial growth. An essential element of epicardial behavior epithelial mesenchymal transition (epiMT), which initial step become motile invade myocardium. To identify targets optimize epicardium-driven repair it vital understand pathways are involved in regulation epiMT. Therefore, we established...
Abstract Background: Recent consensus on molecular classification categorizes colorectal cancer (CRC) into 4 robust subtypes: CMS1 (epithelial-MSI), CMS2 (epithelial-canonical), CMS3 (epithelial-metabolic) and CMS4 (mesenchymal)1. is linked to poor prognosis characterized by mesenchymal epithelial-to-mesenchymal transition (EMT) gene expression2,3. attempts deconvolute the transcriptome from CRC tumors have suggested that expression results a large stromal compartment not due epithelial...
Abstract Background The outside mesothelial layer of the heart, epicardium, is essential during cardiac development. Cells from this undergo epithelial-to-mesenchymal transition (EMT), migrate into tissue and partake in cardiogenesis by contributing cells secretion growth factors. In adult epicardium quiescent but upon ischemic injury it activated conveys a regenerative response. This activation important for repair, suboptimal compared to cardiogenic potential Understanding processes that...
after, and 24h post cell delivery.In vitro bioluminescence signal was used to identify tissue samples containing GFP-Luc-MSCs.Myocardial matrix metalloproteinase 2 (MMP2) (index of ischemic/oxidative stress) CXCR4 receptor expression homing signal) were measured in positive negative myocardial areas one day transfer.Biodistribution the implanted cells quantified by using Luciferase assay confirmed fluorescence immunochemistry.Global left ventricular ejection fraction (LVEF) at baseline month...
Introduction: -Protein quality is assured at a cellular level by finely tuned biological machinery called unfolded protein response (UPR) devoted to recognize, unfold, and refold abnormally arranged proteins.A growing set of previously unrelated disorders has been associated adverse effects deriving from the accumulation non-functional aggregates proteins named oligomers.Those can perturb homeostasis, ultimately survival cell itself.As far as heart concerned, an elevated oligomer presence...
Abstract Background The autonomic nerve system is essential to maintain homeostasis in the body. In heart, innervation important for adjusting physiology continuously changing demands such as stress responses. After cardiac damage, excessive neurite outgrowth, referred hyperinnervation, can occur which related ventricular arrhythmias and sudden death. cellular basis this hyperinnervation yet unresolved. Here we hypothesize a role epicardium derived cells (EPDCs) stimulating sympathetic...
Abstract Background The epicardium, the outer layer of heart, is an indispensable source cells and paracrine factors during embryonic heart formation. In adult epicardium quiescent unless there injury. Cardiac damage results in partial recapitulation developmental processes including epithelial-to-mesenchymal transition (EMT), expression Wilms' Tumor-1 (WT1), proliferation, migration epicardial-derived (EPDCs). Aim Given their vital role development, EPDCs represent appealing for endogenous...
Abstract Background Since the adult heart has minimal capacity to repair itself, myocardial infarction often leads pathological remodelling and consequently development of failure. Unfortunately, no therapy is available restore damaged tissue. The epicardium, outer layer heart, plays an important role during via contribution cells paracrine factors myocardium. In this process, epicardial epithelial-to-mesenchymal transition (EMT) essential step. developmental behaviour recapitulated upon...
Abstract Background The heart is covered by the epicardium, consisting of epithelial cells and a mesenchymal layer. epicardium has been shown to be essential during cardiac development contributing through epithelial-to-mesenchymal transition (EMT) secretion paracrine factors. In adult, conveys cardioprotective response after myocardial infarction, albeit suboptimal compared epicardial contribution development. Although developing characterised in mice zebrafish, knowledge on human fetal...