A5035211563- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
- Digestive system and related health
- Radiation Therapy and Dosimetry
- Immune Cell Function and Interaction
- DNA Repair Mechanisms
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Mathematical Biology Tumor Growth
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Cell Image Analysis Techniques
- Osteoarthritis Treatment and Mechanisms
- NF-κB Signaling Pathways
- RNA Interference and Gene Delivery
- Gastric Cancer Management and Outcomes
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Rheumatoid Arthritis Research and Therapies
University of Amsterdam
2016-2025
Oncode Institute
2017-2025
Amsterdam University Medical Centers
2013-2025
Cancer Center Amsterdam
2008-2025
Amsterdam UMC Location University of Amsterdam
2013-2023
Amsterdam UMC Location Vrije Universiteit Amsterdam
2023
Leiden University Medical Center
1999-2022
Sidra Medical and Research Center
2022
University Medical Center Utrecht
2015-2022
Radboud University Nijmegen
2022
Colon carcinoma is one of the leading causes death from cancer and characterized by a heterogenic pool cells with distinct differentiation patterns. Recently, it was reported that population undifferentiated primary tumor, so-called stem (CSC), can reconstitute original tumor on xenotransplantation. Here, we show spheroid cultures these colon CSCs contain expression CD133, CD166, CD44, CD29, CD24, Lgr5, nuclear beta-catenin, which have all been suggested to mark (cancer) cell population....
Betulinic Acid (BetA) and its derivatives have been extensively studied in the past for their anti-tumor effects, but relatively little is known about precursor Betulin (BE). We found that BE induces apoptosis utilizing a similar mechanism as BetA prevented by cyclosporin A (CsA). cell death more rapidly compared to BetA, achieve amounts of considerably higher concentration needed. Interestingly, we observed cholesterol sensitized cells BE-induced apoptosis, while there was no effect when...
Induction of apoptosis by the cell surface receptor CD95 (APO-1/Fas) has been shown to involve activation a family cysteine proteases (caspases). Recently, new member this identified, designated FLICE (caspase-8/MACH/Mch5). is part death-inducing signaling complex and therefore most upstream caspase in apoptotic pathway. A total eight different isoforms (caspase-8/a–h) have described. To determine which are expressed cells we generated panel monoclonal antibodies directed against all...
Colon cancer stem cells (CSC) can be identified with AC133, an antibody that detects epitope on CD133. However, recent evidence suggests expression of CD133 is not restricted to CSCs, but also expressed differentiated tumor cells. Intriguingly, we observed detection the AC133 cell surface decreased upon differentiation CSC in a manner correlated loss clonogenicity. this event did coincide change promoter activity, mRNA, splice variant, protein expression, or even In contrast, noted...
The metastatic process of colorectal cancer (CRC) is not fully understood and effective therapies are lacking. We show that activation NOTCH1 signaling in the murine intestinal epithelium leads to highly penetrant metastasis (100% metastasis; with >80% liver metastases) Kras
Abstract In colorectal cancer (CRC), a subpopulation of tumor cells, called stem cell (CSC) fraction, is suggested to be responsible for initiation, growth, and metastasis. The search reliable marker identify these CSCs ongoing as current markers, like CD44 CD133, are more broadly expressed therefore not highly selective currently also lack function in CSC biology. Here, we analyzed whether the Wnt target Lgr5, which has earlier been identified murine intestinal could potentially serve...