A5075941702- Transplantation: Methods and Outcomes
- Tracheal and airway disorders
- Organ Transplantation Techniques and Outcomes
- Lung Cancer Diagnosis and Treatment
- Renal Transplantation Outcomes and Treatments
- Pleural and Pulmonary Diseases
- Esophageal Cancer Research and Treatment
- Mechanical Circulatory Support Devices
- Medical Imaging and Pathology Studies
- Esophageal and GI Pathology
- Polyomavirus and related diseases
- Cancer Immunotherapy and Biomarkers
- Sarcoma Diagnosis and Treatment
- Cancer Diagnosis and Treatment
- Metastasis and carcinoma case studies
- Immunotherapy and Immune Responses
- Gastric Cancer Management and Outcomes
- Organ and Tissue Transplantation Research
- Parathyroid Disorders and Treatments
- Congenital Diaphragmatic Hernia Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Soft tissue tumor case studies
- Trauma Management and Diagnosis
- SARS-CoV-2 and COVID-19 Research
University Hospital in Motol
2016-2025
Charles University
2016-2025
Ocní klinika
2004-2023
Creative Commons
2022
Third Way
2018
University Hospital Kralovske Vinohrady
2014
General University Hospital in Prague
2011
Universitätsklinik für Chirurgie Wien
2001
The immunogenicity of the novel mRNA COVID-19 vaccine in immunocompromised lung transplant recipients is still unknown. We compared antibody response after first and second doses BNT162b2 (Pfizer-BioNTech) with natural SARS-CoV-2 infection recipients. None vaccinees tested two developed anti-SARS-CoV-2 IgG, while 85% patients presented an infection. absence to vaccination led us investigate cellular a subset patients. detected specific T-cells 4 out 12 Some therefore might have clinical...
Abstract Intratumoral tertiary lymphoid structures (TLSs) have been associated with improved outcome in various cohorts of patients cancer, reflecting their contribution to the development tumor-targeting immunity. Here, we demonstrate that high-grade serous ovarian carcinoma (HGSOC) contains distinct immune aggregates varying degrees organization and maturation. Specifically, mature TLSs (mTLS) as forming only 16% HGSOCs relatively elevated tumor mutational burden (TMB) are an increased...
To evaluate the safety and effectiveness of novel biodegradable (BD) stents to treat bronchial anastomotic stenosis in patients after lung transplantation.Twenty BD were implanted endoscopically six (median age 41.5 years (range 35-57 years)) with post-transplant stenoses, between 2006 2010. All custom-made from bio-absorbable polydioxanone (PDS). The median stent diameter was 12 mm (8-17 mm) length 20mm (12-30 mm). evaluated clinically, by bronchoscopy high-definition computed tomography...
To predict risk factors of a prolonged air leak following pulmonary lobectomy.From January 2003 to March 2004, 134 patients (95 male, mean age 66+/-7.7 years) underwent lobectomy for lung tumor. Prolonged (PAL) was defined as an lasting 7 days or more postoperative chest tube drainage. Chronic obstructive disease (COPD) FEV1 <70% predicted and FEV1/FVC ratio based on the results preoperative function test.PAL occurred in 13 (9.7%) it lasted 10.4+/-3.2 days. Eleven out with PAL were...
There seems to be a decreased anastomotic leak rate and late stricture formation after linear-stapled (LS) cervical oesophagogastric anastomosis compared with hand-sewn (HS) technique. The aim of our study was compare the surgical outcomes intrathoracic side-to-side LS end-to-end HS transthoracic oesophagectomy. We conducted retrospective review all patients undergoing Ivor Lewis oesophagectomy or for neoplasia at institution from 2005 2012. Anastomotic radiologically clinically graded as...
The presence of circulating tumor cells (CTCs) in patients with metastatic carcinoma is generally associated poor clinical outcome. There have been many investigations showing a possible use CTCs as minimally invasive predictive and prognostic biomarker cancer medicine. In this report size-based method (MetaCell ® ) for quick easy enrichment cultivation presented to enable esophageal (EC) management. total, 43 diagnosed EC, 20 adenocarcinoma (AdenoCa) 23 squamous cell (SCC), were enrolled...
Circulating tumor cells (CTCs) are important targets for treatment and critical surrogate markers when evaluating cancer prognosis therapeutic response. A sensitive methodology detecting CTCs in gastric (GC) patients is needed. In this study we demonstrate a device enrichment cultivation of CTCs. total, 22 with GC, all candidates surgery, were enrolled the study. Peripheral blood samples collected before re-evaluated within operation divided into two groups: resectable non-resectable GC. new...
Background: Extracorporeal membrane oxygenation (ECMO) is frequently used during lung transplantation. Unfractionated heparin (UFH) mainly as part of ECMO support for anticoagulation. One the most common perioperative complications bleeding, which high-dose UFH can aggravate. Methods: We retrospectively analyzed ( n = 141) patients who underwent transplantation between 2020 and 2022. All subjects 109) central cannulated VA with successful intraoperative weaning. Patients on bridge,...
Scoliotic deformity represents a serious spinal disorder that influences the locomotive and cardiopulmonary systems. Some patients with severe scoliosis end-stage lung disease are therefore denied transplantation. In considering transplantation, size match, straight back syndrome, delayed chest closure bronchial stenosis key issues clinicians should evaluate. Therefore, it is vital to determine donor-recipient matches very precisely. Chest opening routine intraoperative primary therapeutic...
The preservation of donor lungs and the effort to safely extend ischemic time while maintaining function is an important topic that transplant community has been addressing for a long time. Recent publications, mainly from Toronto team, have fundamentally influenced existing standard optimal conditions, their results provide scientific basis shift ice controlled hypothermia. Optimal conditions are necessary prerequisite safe extension This brings additional potential development field...
Abstract Background Pulmonary hernia is a rare condition characterized by the protrusion of lung tissue through chest wall defect. Trauma and thoracic surgery are most common causes acquired hernias. We present an unusual case (sequential) bilateral herniation with parenchymal infarction after lobar transplantation. Case presentation A 50-year-old female, wait-listed as high-urgency candidate, body mass index (BMI) 29 kg/m 2 underwent transplantation for pulmonary fibrosis clamshell...
The immunogenicity of the mRNA coronavirus disease 2019 vaccine in thoracic organ transplant recipients is poor.1,2 Early reports provided evidence increased after third dose solid recipients.3,4 However, antibody and cellular responses BNT162b2 (Pfizer-BioNTech) its safety lung (LTRs) are unknown to date. We included 15 LTRs without a history severe acute respiratory syndrome 2 (SARS-CoV-2) infection who received doses 21 d apart with no response. In this cohort, we assessed immediately...
Abstract Background Primary graft dysfunction (PGD) after lung transplantation (LuTx) contributes substantially to early postoperative morbidity. Both intraoperative transfusion of a large amount blood products during the surgery and ischemia–reperfusion injury allograft implantation play an important role in subsequent PGD development. Methods We have previously reported randomized clinical trial 67 patients where point care (POC) targeted coagulopathy management administration 5% albumin...
Introduction Compared with traditional static ice storage, controlled hypothermic storage (CHS) at 4–10°C may attenuate cold-induced lung injury between procurement and implantation. In this study, we describe the first European transplant (LTx) experience a portable CHS device. Methods A prospective observational study was conducted of all consecutively performed LTx following (11 November 2022 31 January 2024) two high-volume centers. The LUNGguard device used for CHS. preservation...
Myasthenia gravis (MG) is an autoimmune disease interfering with neuromuscular transmission. Patients are at risk of postoperative residual curarization (PORC) if nondepolarizing muscle relaxants used. Clinically inapparent insufficient strength may result in hypoventilation and bronchopneumonia. We describe a cohort 117 cases which sugammadex was used MG patients undergoing surgery relaxation rocuronium.We anesthetized using rocuronium as blockade reversal agent. One hundred five underwent...
Transplanted lungs suffer worse outcomes than other organ transplants with many developing chronic lung allograft dysfunction (CLAD), diagnosed by physiologic changes. Histology of transbronchial biopsies (TBB) yields little insight, and the molecular basis CLAD is not defined. We hypothesized that gene expression in TBBs would reveal nature distinguish from changes due simply to time posttransplant. Whole-genome mRNA profiling was performed microarrays 498 prospectively collected INTERLUNG...
High hydrostatic pressure (HHP) induces immunogenic death of tumor cells which confer protective anti-tumor immunity in vivo. Moreover, DC pulsed with HHP-treated induced therapeutic effect mouse cancer model. In this study, we tested the immunogenicity, stability and T cell stimulatory activity human monocyte-derived dendritic (DC)-based HHP lung vaccine generated GMP compliant serum free medium using 250 MPa. HHP-killed poly(I:C) enhanced maturation, chemotactic migration production...