A5021206531- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Ovarian cancer diagnosis and treatment
- Vasculitis and related conditions
- Immunodeficiency and Autoimmune Disorders
- Cancer Mechanisms and Therapy
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Prostate Cancer Treatment and Research
- Renal Diseases and Glomerulopathies
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Sarcoidosis and Beryllium Toxicity Research
- Galectins and Cancer Biology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Phagocytosis and Immune Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glycosylation and Glycoproteins Research
- Erythropoietin and Anemia Treatment
- Otitis Media and Relapsing Polychondritis
- Hematopoietic Stem Cell Transplantation
- Urticaria and Related Conditions
- Virus-based gene therapy research
University Hospital in Motol
2016-2025
Charles University
2016-2025
Institute of Immunology
1998-2024
Sotio (Czechia)
2014-2023
Zdravstveni dom dr. Adolfa Drolca Maribor
2023
Weatherford College
2020-2021
Rockefeller University
2005
Thomayer University Hospital
2005
Institute of Haematology and Blood Transfusion
2005
Prague University of Economics and Business
2003
Immunogenic cell death is characterized by the early surface exposure of chaperones including calreticulin and HSPs, which affect dendritic (DC) maturation uptake presentation tumor antigens. It has also been shown that it late release high mobility group box 1 (HMGB1), acts through Toll-like receptor 4 (TLR4) augments antigens from dying cells to DCs. Most data on immunogenic were obtained using mouse models. In this study, we investigated capacity clinically used chemotherapeutics induce...
Impaired Fas-induced apoptosis of lymphocytes in vitro is a principal feature the autoimmune lymphoproliferative syndrome (ALPS). We studied six children with ALPS whose had normal sensitivity to vitro.
Recent studies have identified molecular events characteristic of immunogenic cell death (ICD), including surface exposure calreticulin (CRT), the heat shock proteins HSP70 and HSP90, release high‐mobility group box protein 1 (HMGB1) ATP from dying cells. We investigated potential high hydrostatic pressure (HHP) to induce ICD in human tumor HHP induced rapid expression HSP70, HSP90 CRT on surface. also HMGB1 ATP. The interaction dendritic cells (DCs) with HHP‐treated led a more rate DC...
// Michal Podrazil 1, * , Rudolf Horvath 7, Etienne Becht 4, 5, 6 Daniela Rozkova 2 Pavla Bilkova Klara Sochorova Hana Hromadkova 1 Jana Kayserova Katerina Vavrova Jan Lastovicka Petra Vrabcova Kubackova 3 Zdenka Gasova 8 Ladislav Jarolim 9 Marek Babjuk Radek Spisek Jirina Bartunkova Jitka Fucikova Department of Immunology, Charles University, 2nd Faculty Medicine and University Hospital Motol, Prague, Czech Republic Sotio, Oncology, 4 Institut National de la Santé et Recherche...
<h3>Importance</h3> DCVAC/PCa is an active cellular immunotherapy designed to initiate immune response against prostate cancer. <h3>Objective</h3> To evaluate the efficacy and safety of plus chemotherapy followed by maintenance treatment in patients with metastatic castration-resistant cancer (mCRPC). <h3>Design, Setting, Participants</h3> The VIABLE double-blind, parallel-group, placebo-controlled, phase 3 randomized clinical trial enrolled mCRPC among 177 hospital clinics US Europe between...
Background Most patients with epithelial ovarian cancer (EOC) relapse despite primary debulking surgery and chemotherapy (CT). Autologous dendritic cell immunotherapy (DCVAC) can present tumor antigens to elicit a durable immune response. We hypothesized that adding parallel or sequential DCVAC CT stimulates antitumor immunity improves clinical outcomes in EOC. Based on the interim results of DCVAC/OvCa administration accommodate increased interest maintenance treatment EOC, trial was...
The successful implementation of immune checkpoint inhibitors (ICI) in the clinical management various solid tumors has raised considerable expectations for patients with epithelial ovarian carcinoma (EOC). However, EOC is poorly responsive to ICIs due immunologic features including limited tumor mutational burden (TMB) and poor lymphocytic infiltration. An autologous dendritic cell (DC)-based vaccine (DCVAC) recently been shown be safe significantly improve progression-free survival (PFS) a...
Abstract The type of immune cells that are present within the tumor microenvironment can play a crucial role in survival patients. However, little is known about dynamics tumor‐infiltrating during disease progression. We studied infiltrated tissues ovarian cancer patients at different stages disease. early development carcinomas were characterized by strong Th17 response, whereas stage II patients, recruitment high numbers Th1 was observed. In disseminated tumors (Stages III–IV), we detected...
Abstract Upregulated Wnt/β-catenin signaling is associated with increased cancer cell resistance and cell-elicited immunosuppression. In non-neoplastic immune cells, upregulated is, however, either immunosuppression or immunostimulation. Therefore, it difficult to predict the therapeutic impact inhibitors of will have when combined immunotherapy. Here, we evaluated benefit(s) inhibitor XAV939 in vitro elimination LNCaP prostate cells cocultured lymphocytes from patients localized...
Background Prostate cancer (PCa) is one of the most common cancers in men. As a “cold tumor,” it exhibits low immunogenicity. Therefore, dendritic cells vaccines (DC) have been developed to enhance adaptive immunity. However, efficacy ex vivo-produced DC remains suboptimal. The antimicrobial peptide LL-37 has shown various immunomodulatory effects. Our previous studies demonstrated that enhances performance produced from peripheral blood healthy volunteers. Aims In this follow-up study, we...
Chronic graft-versus-host disease (cGVHD) represents a major cause of morbidity and mortality after allogeneic bone marrow transplantation (BMT). Extracorporeal photochemotherapy (ECP), technique used in the treatment cutaneous T-cell lymphoma, has also shown clinical efficacy refractory cGVHD.In this study, dynamics dendritic cell (DC) activation were investigated during process photopheresis patients treated for cGVHD.It is reported that myeloid DCs can be isolated from photopheretic...
Abstract Toll‐like receptor (TLR) stimulation plays a crucial role in the homeostasis of human B cells. We investigated expression TLRs 1–9 on cells B‐cell chronic lymphocytic leukemia (B‐CLL) and analyzed functional consequences TLR leukemic showed that B‐CLL express similar set as memory healthy donors, i.e . TLR‐1, TLR‐2, TLR‐6, TLR‐7 TLR‐9. However, contrast to cells, lack TLR‐4 expression. Expression correlates with their capacity respond specific agonists. At level phenotype, ODN2006...
Abstract Background For clinical applications, dendritic cells (DCs) need to be generated using GMP-approved reagents. In this study, we tested the characteristics of DCs in two grade culture media and activated by three maturation stimuli, Poly I: C, LPS mixture proinflammatory cytokines order identify optimal combination activation stimulus for use. Method We generation GMP-certified media, CellGro RPMI+5% human AB serum evaluated morphology, viability capapability mature. PolyI:C,...