A5023684480- Immunotherapy and Immune Responses
- Dendrimers and Hyperbranched Polymers
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Cervical Cancer and HPV Research
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS Research and Interventions
- Telomeres, Telomerase, and Senescence
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Carbohydrate Chemistry and Synthesis
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
Czech Academy of Sciences, Institute of Molecular Genetics
2016-2025
Czech Academy of Sciences
2008-2024
Institute of Molecular Genetics
2012
New York State Department of Health
2006-2008
Wadsworth Center
2006-2007
University Surgical Associates
2002
National Institute of Public Health
2001
University of Turin
1998
While type I interferon (IFN) is best known for its key role against viral infection, accumulating preclinical and clinical data indicate that robust IFN production in the tumor microenvironment promotes cancer immunosurveillance contributes to efficacy of various antineoplastic agents, notably immunogenic cell death inducers. Here, we report malignant blasts from patients with acute myeloid leukemia (AML) release via a Toll-like receptor 3 (TLR3)-dependent mechanism not driven by treatment....
Summary Epigenetic events play an important role in tumour progression and also contribute to escape of the from immune surveillance. In this study, we investigated up‐regulation major histocompatibility complex (MHC) class I surface expression on cells by epigenetic mechanisms using a murine cell line expressing human E6 E7 papilloma virus 16 (HPV16) oncogenes deficient MHC expression, as result impaired antigen‐presenting machinery (APM). Treatment with histone deacetylase inhibitor...
In some settings, cancer cells responding to treatment undergo an immunogenic form of cell death that is associated with the abundant emission danger signals in damage-associated molecular patterns. Accumulating preclinical and clinical evidence indicates play a crucial role (re-)activation antitumor immune responses vivo, thus having major impact on patient prognosis. We have previously demonstrated presence calreticulin surface malignant blasts positive prognostic biomarker for patients...
Nanrilkefusp alfa (nanril; SOT101) is an interleukin (IL)-15 receptor βγ superagonist that stimulates natural killer (NK) and CD8+ T cells, thereby promoting innate adaptive anti-tumor inflammatory microenvironment in mouse tumor models either monotherapy or combined with anti-programmed cell death protein 1 (PD-1) antibody. In cynomolgus monkeys, a clinical schedule was identified, which translated into the design of phase 1/1b trial, AURELIO-03 (NCT04234113). 51 patients...
Background Prostate cancer (PCa) is one of the most common cancers in men. As a “cold tumor,” it exhibits low immunogenicity. Therefore, dendritic cells vaccines (DC) have been developed to enhance adaptive immunity. However, efficacy ex vivo-produced DC remains suboptimal. The antimicrobial peptide LL-37 has shown various immunomodulatory effects. Our previous studies demonstrated that enhances performance produced from peripheral blood healthy volunteers. Aims In this follow-up study, we...
ABSTRACT MDSCs represent one of the key players mediating immunosuppression. These cells accumulate in TME, lymphoid organs, and blood during tumor growth. Their mobilization was also reported after CY therapy. DNMTi 5AC has been intensively studied as an antitumor agent. In this study, we examined, using two different murine models, modulatory effects on TU-MDSCs CY-MDSCs growth Indeed, percentage TME spleens 5AC-treated mice bearing TRAMP-C2 or TC-1/A9 tumors found decreased. The changes...
Myeloid-derived suppressor cells (MDSC) play an important role in tumor escape from antitumor immunity. MDSC accumulate the lymphoid organs and blood during growth their mobilization was also reported after cyclophosphamide (CY) administration. In this communication, spleen accumulating CY therapy (CY-MDSC) were compared with those expanded mice bearing human papilloma viruses 16-associated TC-1 carcinoma (TU-MDSC). Although both CY-MDSC TU-MDSC accelerated of tumors vivo, phenotype...
Signal transducer and activator of transcription 3 (STAT3) signalling serves an important role in carcinogenesis cellular senescence, its inhibition tumour cells represents attractive therapeutic target. Premature a process permanent proliferative arrest response to various inducers, such as cytostatic drugs or ionizing radiation, is accompanied by morphological secretory changes, altered susceptibility chemotherapeutic agents, which can thereby complicate their eradication cancer therapies....
Epigenetic mechanisms have important roles in the tumour escape from immune responses, such as MHC class I downregulation or altered expression of other components involved antigen presentation. Chemotherapy with DNA methyltransferase inhibitors (DNMTi) can thus influence cell interactions system and their sensitivity to immunotherapy. We evaluated therapeutic effects DNMTi 5-azacytidine (5AC) against experimental I-deficient -positive tumours. The 5AC therapy was combined immunotherapy,...
// Veronika Vlková 1 , Ivan Štěpánek Hrušková Filip Šenigl 2 Mayerová Martin Šrámek Jana Šímová Bieblová Marie Indrová Tomáš Hejhal Nicolas Dérian 3,4,5,6 David Klatzmann Adrien Six and Milan Reiniš Department of Tumour Immunology, Institute Molecular Genetics, Academy Sciences the Czech Republic, v. i., Prague Viral Cellular 3 Sorbonne Universités, UPMC Univ Paris 06, UMR 7211, Immunology-Immunopathology-Immunotherapy (I3), Paris, France 4 CNRS, FRE 3632, 5 INSERM, UMR_S 959, 6 AP-HP,...
High hydrostatic pressure (HHP) has been shown to induce immunogenic cell death of cancer cells, facilitating their uptake by dendritic cells (DC) and subsequent presentation tumor antigens. In the present study, we demonstrated immunogenicity HHP-treated in mice. HHP was able both TC-1 TRAMP-C2 representing murine models for human papilloma virus-associated tumors prostate cancer, respectively. induced stronger immune responses mice immunized with these documented higher spleen cytotoxicity...
Abstract The endocytosis of the human CD38 molecule has been investigated in normal lymphocytes and a number leukemia- lymphoma-derived cell lines. internalization was followed using radioiodinated Abs an acidic elution assay to monitor effects cross-linking on processes quantify ratio internalized molecule. Second, conventional, confocal, electron microscopies were used evaluate morphologic induced by ligation with mimicking natural ligand(s). results demonstrated that is reproducible...
Myelodysplastic syndromes (MDS) represent a heterogeneous group of clonal stem cell disorders characterized by ineffective hematopoiesis frequently progressing into acute myeloid leukemia (AML), with emerging evidence implicating aberrant bone marrow (BM) microenvironment and inflammation-related changes. 5-azacytidine (5-AC) represents standard MDS treatment. Besides inhibiting DNA/RNA methylation, 5-AC has been shown to induce DNA damage apoptosis in vitro. To provide insights vivo...
As a part of cellular pathogen defense, IFNγ triggers induction NADPH oxidase NOX2, which produces superoxide into phagosomes immune cells. Recent data show that similar mechanism can also operate in IFNγ-mediated anticancer control. is capable inducing expression constitutively active NOX4 tumor cells leading to generation reactive oxygen species (ROS) damaging DNA, activation DNA damage response and cell cycle arrest/premature senescence.
Standard-of-care chemo- or radio-therapy can induce, besides tumor cell death, also senescence. While senescence is considered to be a principal barrier against tumorigenesis, senescent cells survive in the organism for protracted periods of time and they promote development. Based on this emerging concept, we hypothesized that elimination such potentially cancer-promoting could offer therapeutic benefit. To assess possibility, here first show growth proliferating mouse TC-1...
Nanoparticles of an Mo 6 cluster compound exert a robust radiosensitizing activity against TRAMP-C2 cells, thanks to their ability produce singlet oxygen upon X-ray irradiation.
Abstract Chronic inflammation represents a major threat to human health since long‐term systemic is known affect distinct tissues and organs. Recently, solid evidence demonstrated that chronic affects hematopoiesis; however, how hematopoietic stem cells (HSCs) on the mechanistic level poorly understood. Here, we employ mouse model of multifocal osteomyelitis (CMO) assess effects spontaneously developed inflammatory condition HSCs. We demonstrate nonhematopoietic compartments in CMO BM...
Dendritic cell (DC)-based vaccines pulsed with high hydrostatic pressure (HHP)-inactivated tumor cells have recently been shown to be a promising tool for prostate cancer chemoimmunotherapy. In this study, DC-based vaccines, both and unpulsed, were as effective docetaxel (DTX) in reducing tumors the orthotopic transgenic adenocarcinoma of mouse (TRAMP) model. However, we did not observe any additive or synergic effects chemoimmunotherapy on growth, while only combination DTX dendritic...