A5009917845- Calcium signaling and nucleotide metabolism
- Monoclonal and Polyclonal Antibodies Research
- Adenosine and Purinergic Signaling
- Signaling Pathways in Disease
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Systemic Sclerosis and Related Diseases
- Toxin Mechanisms and Immunotoxins
- Cell Adhesion Molecules Research
- Ion Channels and Receptors
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- DNA Repair Mechanisms
- Chronic Lymphocytic Leukemia Research
- Eosinophilic Disorders and Syndromes
- Protein purification and stability
- Complement system in diseases
- PARP inhibition in cancer therapy
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Immunodeficiency and Autoimmune Disorders
- Mitochondrial Function and Pathology
- Graphene and Nanomaterials Applications
- Systemic Lupus Erythematosus Research
- Cutaneous lymphoproliferative disorders research
University of Turin
2016-2025
Institut d'Investigació Biomédica de Bellvitge
2012
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2012
Ospedale Sant'Anna
2010
Azienda Ospedaliero Universitaria San Giovanni Battista
2003-2007
Torino e-district
2003
San Raffaele University of Rome
1994
Institute of Cell Biology and Neurobiology
1993
Ospedale Regina Margherita
1993
IRCCS Ospedale San Raffaele
1993
Systemic sclerosis (scleroderma) is characterized by immunologic abnormalities, injury of endothelial cells, and tissue fibrosis. Abnormal oxidative stress has been documented in scleroderma linked to fibroblast activation. Since platelet-derived growth factor (PDGF) stimulates the production reactive oxygen species (ROS) since IgG from patients with reacts human fibroblasts, we tested hypothesis that have serum autoantibodies stimulate PDGF receptor (PDGFR), activating collagen-gene expression.
Event Abstract Back to CD157-extracellular matrix proteins interactions enhance integrin-mediated signalling cascade in monocytes. Simona Morone1*, Stefania Augeri1, Alice Giacomino1, Rossella Parrotta1, Nicola Lo Buono1, Massimiliano Cuccioloni2, Erika Ortolan1 and Ada Funaro1 1 University of Torino, Department Medical Sciences, Italy 2 Camerino, School Biosciences Biotechnology, CD157 is a GPI-anchored ectoenzyme belonging the NADase/ADP-ribosyl cyclase gene family involved control human...
Ghrelin is a novel gastrointestinal hormone produced by rat and human gastric X-like neuroendocrine cells, which strongly stimulates GH secretion influences energy balance, motility, acid secretion. expressed in pituitary endocrine tumors. It binds to the secretagogue receptor (GHS-R), present wide variety of central peripheral tissues. The aim study was 2-fold: 1) determine, immunohistochemistry mRNA analysis, whether pancreatic islet cells produce ghrelin express GHS-R; 2) investigate...
We report clear evidence that the interaction of CD38 molecule with specific mAb A10 on normal human cells and lines modulates expression surface activation markers relevant to T, NK, plasma cell biology functions. Moreover binding is followed by proliferation effects all target analyzed, phenomenon accessory IL-2 dependent. The synergizing both CD2 CD3 pathways indicate signal transduction mechanism(s) are apparently different from aforementioned. Nevertheless decreased A10-driven after...
Abstract CD38 is a type II transmembrane glycoprotein, which widely used as marker for immature and activated lymphocytes, well plasma cells. Although its functional role natural ligand are not known, has been shown to transduce activation signals lymphocytes. Our work shows that preferentially expressed by CD4+CD45RA+ cells, but CD4+CD45R0+ cells reported respond poorly stimuli acting through the CD3/TCR in vitro display unique migration pathways vivo. Cross-linking of mAb did overcome...
Abstract The structure of the CD38 molecule has been evaluated by one- and two-dimensional gel analysis enzymatic digestions. source Ag was mainly membrane preparations obtained from MLC cells, normal thymocytes, plasmocytoma line LP-1. Membranes were solubilized in NP-40 extracts fractionated immunoaffinity chromatography [using a specific anti-CD38 antibody (A10 mAb) covalently linked to Sepharose protein A]. purified migrated as single chain Mr = 45,000 not associated with beta...
The CD38 antigen displays restricted functional associations with surface molecules involved in immune system and complement. Capping of the molecule normal or neoplastic T cells is followed by rapid specific co-modulation CD3-T cell receptor (TcR) complex. In tumor B lineage, was found to be also associated Ig (sIg) complement 2 (CR2)/CD19 expressed purified natural killer displayed an association low affinity IgG Fc (Fc gamma RIII) CD16. These observations suggest that specialized areas...
Abstract Human CD38, a pleiotropic molecule with ADP-ribosyl cyclase activity, regulates activation and growth of several cell types. Its in vivo function is incompletely determined, mainly due to the lack evidence concerning existence single or multiple ligands. We recently observed that CD38 rules selectin-type adhesion between lymphoid cells HUVECs. A panel murine mAbs raised against HUVEC included one (Moon-1) constantly blocking CD38-mediated lines HUVEC. Tissue distribution studies an...
Human CD38 is a signal transduction molecule, and, concurrently, an ectoenzyme catalyzing the synthesis and degradation of cyclic ADP-ribose (cADPR), potent Ca2+ mobilizer. One facet that has not yet been addressed its role in NK cells. To this end, events triggered by ligation with agonistic mAb were analyzed on freshly purified human Ligation was followed (i) significant rise intracellular level Ca2+, (ii) increased expression HLA class II CD25, (iii) tyrosine phosphorylation discrete...
Abstract Human CD38 is a surface glycoprotein expressed by different immuno‐competent cells such as immature and activated lymphocytes, plasma natural killer cells. It has recently been reported that the molecule exerts adenosine diphosphate ribosyl cyclase activity associated with distinct transmembrane signaling molecules. This study reports ligation of specific monoclonal antibodies (mAb) induces multiple cytokine mRNA expression in cultured peripheral blood mononuclear (PBMC). The for...
Objective:Our objective was to perform mutation analysis of the LHR gene patients with Leydig cell adenoma or hyperplasia. Functional studies were conducted compare D578H-LHR mutant wild-type (WT)-LHR and D578G-LHR mutant, a classic cause testotoxicosis. The three main signal transduction pathways in which is involved studied.
CD157, an ADP-ribosyl cyclase-related cell surface molecule, regulates leukocyte diapedesis during inflammation. Because CD157 is expressed in mesothelial cells and resembles tumor migration, we investigated the role of ovarian carcinoma.We assayed surgically obtained cancer both native engineered lines for expression using flow cytometry reverse transcription-polymerase chain reaction (RT-PCR), adhesion to extracellular matrices, invasion cell-based assays. We human peritoneal by serous...
CD157, a member of the CD38 gene family, is an NAD-metabolizing ectoenzyme and signaling molecule whose role in polarization, migration, diapedesis human granulocytes has been documented; however, molecular events underpinning this remain to be elucidated. This study focused on exerted by CD157 monocyte migration across endothelial lining adhesion extracellular matrix proteins. The results demonstrated that anti-CD157 antibodies block transmigration fibronectin fibrinogen but cross-linking...