A5039345319- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Tissue Engineering and Regenerative Medicine
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Education, Psychology, and Social Research
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- Reproductive Biology and Fertility
- Regulation of Appetite and Obesity
- Pluripotent Stem Cells Research
- Biochemical Analysis and Sensing Techniques
- Sperm and Testicular Function
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Angiogenesis and VEGF in Cancer
- Multiple Myeloma Research and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Analytical Methods in Pharmaceuticals
- Bone and Dental Protein Studies
Charles University
2013-2024
Imperial College London
2018
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2011-2014
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2014
Czech Academy of Sciences
2011-2014
United States Department of Agriculture
2001
Czech University of Life Sciences Prague
2000
Abstract Severe influenza infection has no effective treatment available. One of the key barriers to developing host-directed therapy is a lack reliable prognostic factors needed guide such therapy. Here, we use network analysis approach identify host associated with severe and fatal outcome. In patients moderate-to-severe diseases, uncover complex landscape immunological pathways, main changes occurring in pathways related circulating neutrophils. Patients disease display excessive...
Chimeric antigen receptor anti-CD19 (CAR19)-T cell immunotherapy-induced clinical remissions in CD19+ B lymphomas are often short lived. We tested whether CAR19-engineering of the CD1d-restricted invariant natural killer T (iNKT) cells would result enhanced anti-lymphoma activity. CAR19-iNKT co-operatively activated by CD1d- and CAR19-CD19-dependent interactions more effective than CAR19-T against CD1d-expressing vitro vivo. The swifter vivo activity their ability to eradicate brain...
In 2019, the EPMA celebrated its 10th anniversary at 5th World Congress in Pilsen, Czech Republic. The history of International Professional Network dedicated to Predictive, Preventive and Personalised Medicine (PPPM / 3PM) is rich achievements. Facing coronavirus COVID-19 pandemic it getting evident globally that predictive approach, targeted prevention personalisation medical services optimal paradigm healthcare demonstrating high potential save lives benefit society as a whole. Supplement...
Accumulating evidence indicates that immune checkpoint inhibitors (ICIs) can restore CD8+ cytotoxic T lymphocyte (CTL) functions in preclinical models of acute myeloid leukemia (AML). However, ICIs targeting programmed cell death 1 (PDCD1, best known as PD-1) and lymphocyte-associated protein 4 (CTLA4) have limited clinical efficacy patients with AML. Natural killer (NK) cells are central players AML-targeting responses. little is on the relationship between co-inhibitory receptors expressed...
While type I interferon (IFN) is best known for its key role against viral infection, accumulating preclinical and clinical data indicate that robust IFN production in the tumor microenvironment promotes cancer immunosurveillance contributes to efficacy of various antineoplastic agents, notably immunogenic cell death inducers. Here, we report malignant blasts from patients with acute myeloid leukemia (AML) release via a Toll-like receptor 3 (TLR3)-dependent mechanism not driven by treatment....
In some settings, cancer cells responding to treatment undergo an immunogenic form of cell death that is associated with the abundant emission danger signals in damage-associated molecular patterns. Accumulating preclinical and clinical evidence indicates play a crucial role (re-)activation antitumor immune responses vivo, thus having major impact on patient prognosis. We have previously demonstrated presence calreticulin surface malignant blasts positive prognostic biomarker for patients...
Sutherland et al. recently published the Practical Guidelines for high-sensitivity detection of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry (FCM), containing concise protocols PNH testing.Using this approach, we studied intra- and interlaboratory variability observed in a multicenter study which fresh blood samples three clinically relevant clone sizes within granulocytic, monocytic, red cell (RBC) populations were shipped to each participating center.Coefficients...
Abstract Translating insights from single-cell analysis into actionable indicators of health and disease requires large-scale confirmatory studies. We introduce biocytometry, a novel method utilizing engineered bioparticles for multiparametric immunophenotyping in suspension, enabling simultaneous measurement across thousands assays with sensitivity wide dynamic range (1 to 1,000 target cells/sample). The technical validation biocytometry revealed strong alignment established technologies...
Mesenchymal stem cells (MSCs) have been repeatedly shown to be able repair bone defects. The aim of this study was characterize the osteogenic differentiation miniature pig MSCs and markers in vitro. Flow-cytometrically characterized were seeded on cultivation plastic (collagen I vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate (PAC) scaffolds differentiated medium. During three weeks differentiation, formation nodules deposition calcium visualized by Alizarin Red Staining....
Cryopreserved haematopoietic progenitor cells are used to restore autologous haematopoiesis after high dose chemotherapy. Although the routinely stored for a long period, concerns remain about maximum storage time and possible negative effect of on their potency. We evaluated cryopreservation quality peripheral stem cell grafts short (3 months) (10 years) period we compared it native products.The viability CD34+ remained unaffected during storage, apoptotic were represented up 10% did not...
The aim of our study was to investigate the parthenogenetic activation in vitro matured pig oocytes after their combined treatment with calcium ionophore A 23187 and inhibitor protein kinases, 6‐dimethylaminopurine (6‐DMAP) further embryonic development activated using this treatment. were exposed (10, 25 or 50 μ M ) for 0.5, 1, 3, 5 7 min then cultured 6‐DMAP (0 2 highest rate (up 88% eggs reached pronuclear stage) observed 6‐DMAP. without 6‐DMAP, when up 51% developed beyond two‐cell...
Mesenchymal stromal cells (MSC) represent a promising treatment of graft-versus-host disease (GVHD) in patients after allogeneic haematopoietic stem cell transplantation. We performed co-cultivation experiments with non-specifically stimulated lymphocytes to characterize the immunosuppressive activity MSC. MSC influenced expression some activation antigens. CD25 was lower and reached 55.2 % vs. 84.9 (CD4 + , P = 0.0006) 38.8 86.6 (CD8 0.0003) on day +4. Conversely, CD69 antigen remained...