A5028900781- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- Ultrasound and Hyperthermia Applications
- Toxin Mechanisms and Immunotoxins
- Effects of Radiation Exposure
- Ultrasound and Cavitation Phenomena
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Nanoparticle-Based Drug Delivery
- Radiopharmaceutical Chemistry and Applications
- Blood Coagulation and Thrombosis Mechanisms
- Transgenic Plants and Applications
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Medical Imaging and Pathology Studies
- Sperm and Testicular Function
- Advanced biosensing and bioanalysis techniques
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Laser-Plasma Interactions and Diagnostics
- Plant Genetic and Mutation Studies
- Microbial Inactivation Methods
- Spectroscopy Techniques in Biomedical and Chemical Research
Charles University
2014-2024
Czech Academy of Sciences, Institute of Biophysics
2012-2023
Czech Academy of Sciences, Institute of Molecular Genetics
2002-2008
Instituto de Química Física Blas Cabrera
2007
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2007
University of Verona
2007
Institute of Haematology and Blood Transfusion
2000-2006
Ministry of Health
2006
Ministry of Industry and Trade
2005
The University of Texas at Austin
2002
The porphyrin−cyclodextrin conjugates were prepared and tested for selective effective multifunctional drug delivery therapy. porphyrin receptor system combines efficient binding of the selected to cyclodextrin cavity photosensitizing properties moiety with high accumulation whole complex in cancer tissue. combined effect chemotherapy photodynamic therapy is demonstrated by vitro vivo studies.
Abstract Statins are widely used for the treatment of hypercholesterolemia. However, their inhibitory action on HMG‐CoA reductase also results in depletion intermediate biosynthetic products, which importantly contribute to cell proliferation. The aim present study was compare effects individual commercially available statins experimental pancreatic cancer. vitro (pravastatin, atorvastatin, simvastatin, lovastatin, cerivastatin, rosuvastatin and fluvastatin) viability human cancer were...
Sodium valproate (VPA) belongs to the group of simple branched-chain fatty acids and due its anticonvulsive activity is broadly applied in treatment epilepsy. We previously showed that VPA able induce cellular differentiation, enhance immunogenicity inhibit proliferation human neuroblastoma (NB) cells vitro. Furthermore, we demonstrated inhibits proliferation, enhances neural cell adhesion molecule expression decreases CD44 rat glioma In present study investigated antitumoral effects on...
The design and synthesis of glycol-functionalized porphyrins that contain one to four low molecular weight glycol chains are linked via ether bonds the meta-phenyl positions meso-tetraphenylporphyrin comparison fluorinated nonfluorinated para derivatives reported. cellular uptake photodynamic activity significantly depend on terminal groups substituent. Hydroxy porphyrins, in contrast with methoxy show efficient intracellular transport a high induction apoptosis tumor cell lines vitro....
The lack of cellular and tissue specificities in conventional chemotherapies along with the generation a complex tumor microenvironment (TME) limits dosage active agents that reaches sites, thereby resulting ineffective responses side effects. Therefore, development selective TME-responsive nanomedicines is due relevance toward successful chemotherapies, albeit challenging. In this framework, we have synthesized novel, ready-to-use ROS-responsive amphiphilic block copolymers (BCs) two...
Dimers, trimers, and tetramers of bovine ribonuclease A, obtained by lyophilization the enzyme from 40% acetic acid solutions, were purified isolated cation exchange chromatography. The two conformers constituting each aggregated species assayed for their antitumor, aspermatogenic, or embryotoxic activities in comparison with monomeric RNase A seminal RNase, which is dimeric nature. antitumor action was tested vitro on ML-2 (human myeloid leukemia) HL-60 cell line) cells vivo growth human...
Two porphyrin–brucine quaternary ammonium salts were immobilized on gold nanoparticles and their suitability for both in vitro vivo photodynamic therapy (PDT) was assayed using the basaloid squamous cell carcinoma PE/CA-PJ34 line. In PDT experiments revealed that nanoparticle-bound conjugates less effective than unbound killing cells. However, same more reducing tumor size vivo, with complete regression observed.
The synthesis, characterization, and biolocalization properties of new, fully water-soluble sapphyrins is reported. These systems, which display light absorbing features that make them potential interest as photodynamic therapy (PDT) sensitizers, were found to localize selectively in pancreatic carcinoma tissue a xenographic murine model. In this model, the concentration ratios for malignant surrounding muscle be high 280 most favorable case. concentrations reported here, well porphyrin...
This paper describes the preparation and use of conjugates porphyrins bile acids as ligands to bind tumor expressed saccharides. Bile acid–porphyrin were tested for recognition saccharides that are typically present on malignant cells. Fluorescence microscopy, in vitroPDT cell killing, PDT subcutaneous 4T1 mouse tumors is reported. High selectivity saccharide cancer markers cells was observed. vivo vitro study demonstrated high potential these compounds targeted photodynamic therapy.
Proteinase-activated receptor-2 (PAR-2) is a ubiquitous surface molecule participating in many biological processes. It belongs to the family of G protein-coupled receptors activated by site-specific proteolysis trypsin and similar proteases. Altered function PAR-2 has been described different malignant tumors. In present study, we investigated expression breast cancer surgical specimens role cell line MDA MB-231 proliferation metabolism. A total 40 samples infiltrative ductal were included...
Abstract Survivin, an important antiapoptotic protein, is expressed in tumors, whereas normal tissues the expression of this protein extremely low, defining a role for survivin as cancer gene. Survivin exhibits multifunctional activity tumor cells. However, why sharply and invariably restricted to tissue remains unclear. Here, we identified 11 putative consensus binding sites GLI transcription factors promoter characterized activity. Inhibitors Hedgehog/GLI pathway, cyclopamine GANT61,...
ABSTRACT In the present study we investigated photosensitizing properties of two novel mono‐ and bis‐cyclodextrin tetrakis (pentafluorophenyl) porphyrin derivatives in several tumor cell lines BALB/c mice bearing subcutaneously transplanted syngeneic mouse mammary carcinoma 4T1. Both studied sensitizers were localized mainly lysosomes found to induce death by triggering apoptosis human leukemic cells HL‐60. 4T1 other both apoptotic necrotic modes occurred depending on drug light doses....
A novel method for generation of focused shock waves has been developed. cylindrical pressure wave, created by a high-voltage multichannel discharge in water with an increased electrical conductivity, is metallic parabolic reflector. The formed on composite anode consisting stainless steel electrode covered thin porous ceramic layer. In such arrangement at the applied voltage 30 kV, large number short channels distributed homogeneously surface initiated. Each channel creates semi-spherical...
Abstract Photodynamic therapy (PDT) has garnered immense attention as a minimally invasive clinical treatment modality for malignant cancers. However, its low penetration depth and photodamage of living tissues by UV visible light, which activate photosensitizer, limit the application PDT. In this study, monodisperse NaYF 4 :Yb 3+ /Er nanospheres 20 nm in diameter, that serve near‐infrared (NIR)‐to‐visible light converters activators were synthesized high‐temperature co‐precipitation...