A5070480931- Growth Hormone and Insulin-like Growth Factors
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Regulation of Appetite and Obesity
- Diet and metabolism studies
- Bariatric Surgery and Outcomes
- Fibroblast Growth Factor Research
- Pituitary Gland Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Biochemical Analysis and Sensing Techniques
- Lipid metabolism and disorders
- Gestational Diabetes Research and Management
- Adrenal Hormones and Disorders
- Diabetes Treatment and Management
- Systemic Lupus Erythematosus Research
- Metabolism, Diabetes, and Cancer
- Pregnancy and preeclampsia studies
- Kruppel-like factors research
- Bone health and treatments
- Eating Disorders and Behaviors
- Epigenetics and DNA Methylation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Atherosclerosis and Cardiovascular Diseases
- Peroxisome Proliferator-Activated Receptors
Charles University
2014-2023
Institute of Clinical and Experimental Medicine
2017-2023
General University Hospital in Prague
2008-2021
Centre of Experimental Medicine of the Slovak Academy of Sciences
2019
University Hospital Kralovske Vinohrady
1990-2016
Third Way
2008-2014
Czech Academy of Sciences
2002-2003
Czech Academy of Sciences, Institute of Physiology
2002-2003
Ocní klinika
1990
Fibroblast growth factor-21 (FGF21) is a novel endocrine and paracrine regulator of metabolic homeostasis. The aim our study was to measure its serum concentrations in patients with obesity, obesity type 2 diabetes mellitus (T2DM) healthy subjects (C), assess the changes circulating levels mRNA expression after dietary pharmacological interventions.We measured biochemical parameters, FGF21, adiponectin, leptin insulin by commercial ELISA RIA kits, liver, subcutaneous visceral fat RT PCR 26...
Hyperglycemia and insulin resistance frequently occur in critically ill patients even without a history of diabetes.Our objective was to study the role adipose tissue hormonal production development cardiac surgery patients. PARTICIPANTS, INTERVENTIONS, AND SETTINGS: Fifteen with elective underwent blood sampling before, at end, 6, 12, 24, 48, 120 h after end their operation. Epicardial sc done beginning Department Cardiac Surgery.We measured serum concentrations epicardial mRNA expression...
Objective Macrophage inhibitory cytokine-1 (MIC-1) is a novel regulator of energy homeostasis. We explored whether alterations in MIC-1 levels contribute to metabolic disturbances patients with obesity and/or and type 2 diabetes mellitus (T2DM). Design measured serum its mRNA expression subcutaneous visceral adipose tissue 17 obese nondiabetic women, 14 women T2DM 23 healthy lean women. also the relationship anthropometric biochemical parameters studied influence 2-week very low calorie diet...
Reactive oxygen species generated by hyperglycaemia modify structure and function of lipids, proteins other molecules taking part in chronic vascular changes diabetes mellitus (DM). Low activity scavenger enzymes has been observed patients with DM. Protective role may be deteriorated oxidative stress. This study was undertaken to investigate the association between gene polymorphisms selected antioxidant complications DM.Significant differences allele genotype distribution among T1DM, T2DM...
Objective Fibroblast growth factor (FGF)‐19 and FGF‐21 are novel metabolic regulators that improve insulin resistance obesity in rodents. The aim of the study was to assess effects laparoscopic sleeve gastrectomy (LSG) on serum concentrations FGF‐19 along with circulating bile acids other relevant hormonal biochemical parameters. Design Methods Seventeen females undergoing LSG 15 lean healthy were included into study. Anthropometric parameters, ‐21, insulin, adiponectin, leptin, C‐reactive...
Fibroblast growth factor 19 (FGF19) and FGF21 are novel metabolic regulators that improve insulin sensitivity decrease adiposity in mice. However, little is known about the nutritional regulation of these factors humans.The objective this study was to measure plasma FGF19 levels patients with anorexia nervosa (AN) explore its relationship anthropometric endocrine parameters.This a single-center cross-sectional study.The performed university hospital.Seventeen untreated women restrictive type...
Paraoxonase 1 (PON1), an antioxidant enzyme closely associated with HDL (high-density lipoproteins), preserves LDL (low-density lipoproteins) against oxidation. Less protection may be therefore supposed by decreased PON1 activity. This study was undertaken to investigate the association of gene polymorphisms diabetic angiopathy and evaluate relationship these Total 86 Type (T1DM) 246 2 (T2DM) patients together 110 healthy subjects were examined. DNA isolated from leukocytes amplified...
Abstract Background Angiopoietin-like proteins (ANGPTLs) 3 and 4 are circulating factors that participate in the regulation of lipid glucose metabolism. Subjects methods We measured serum ANGPTL3 levels 23 patients with obesity, 40 obesity type 2 diabetes mellitus (T2DM), 22 anorexia nervosa (AN), 15 subjects undergoing 72-h fasting, 12 short bowel syndrome (SBS), their changes after very-low-calorie diet (VLCD), bariatric surgery, partial realimentation, acute parenteral nutrition order to...
Abstract Context: Low-grade inflammation links obesity, type 2 diabetes mellitus (T2DM), and cardiovascular diseases. Objective: To explore the expression profile of genes involved in inflammatory pathways adipose tissue peripheral monocytes (PM) obese patients with without T2DM at baseline after dietary intervention. Design: Two-week intervention study very-low-calorie diet (VLCD). Setting: University hospital. Patients: Twelve females T2DM, 8 nondiabetic (OB) 15 healthy age-matched...
Increased circulating adhesion molecules in patients with obesity play an important role the development of endothelial dysfunction/atherosclerosis. The aim this study was to assess contribution various fat depots production obesity. 12 women 1st and 2nd degree obesity, 13 3rd 14 lean age-matched were included into study. Circulating levels vascular cell molecule-1 (VCAM-1), intercellular (ICAM-1), E-selectin measured by Luminex kits. mRNA expression ICAM-1, VCAM-1, E-selectin, monocyte...
The aim of our study was to measure serum concentrations fibroblast growth factor 19 (FGF-19) in patients with obesity (OB), and type 2 diabetes mellitus (T2DM) healthy subjects (C) at baseline after selected interventions. We measured FGF-19 levels other biochemical hormonal parameters 29 OB T2DM females 30 sex- age-matched control subjects. interventions were acute hyperinsulinemia during isoglycemic-hyperinsulinemic clamp (n=11 for 10 C), very-low calorie diet (VLCD, n=12 OB) 3 months...
Summary Objective To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function adipose tissue both circulating and subcutaneous fat mRNA expression level. Patients design A total 12 18 normal weight age‐matched women underwent anthropometric examination, single blood drawing biopsy. Measurements Serum concentrations high‐sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin‐6 insulin were measured by Luminex,...
Omentin is a novel adipokine with insulin-sensitizing effects expressed predominantly in visceral fat. We investigated serum omentin levels and its mRNA expression subcutaneous adipose tissue (SCAT) of 11 women type 2 diabetes mellitus (T2DM), 37 obese non-diabetic (OB) 26 healthy lean (C) before after various weight loss interventions: 2-week very-low-calorie diet (VLCD), 3-month regular exercise laparoscopic sleeve gastrectomy (LSG). At baseline, both T2DM OB groups had decreased...
Duodenal-jejunal bypass liner (DJBL) is an endoscopically implantable device designed to noninvasively mimic the effects of gastrointestinal operations by excluding duodenum and proximal jejunum from contact with ingested food. The aim our study was assess influence DJBL on anthropometric parameters, glucose regulation, metabolic hormonal profile in obese patients type 2 diabetes mellitus (T2DM) characterize both magnitude possible mechanisms its effect. Thirty poorly controlled T2DM...
We studied the effect of peroxisome proliferator-activated receptor-α (PPAR-α) activation on serum concentrations and tissue expression resistin, adiponectin, adiponectin receptor-1 -2 (AdipoR1 AdipoR2) mRNA in normal mice with insulin resistance induced by lipogenic, simple-carbohydrate diet (LD). Sixteen weeks LD feeding obesity liver steatosis increased levels but did not significantly affect circulating or resistin. Treatment PPAR-α agonist fenofibrate decreased body weight fat pad...
Summary background Growth hormone deficiency (GHD) in adult life has been associated with increased central adiposity, decreased insulin sensitivity, dyslipidaemia and risk of cardiovascular disease. The effects GH replacement on adiponectin resistin, adipokines which have a role modulating sensitivity not previously reported. aim To examine the adipokine levels resistance GHD patients. design Seventeen patients were examined at baseline after 1 year treatment recombinant human (mean dose...
Genes for adiponectin and resistin are candidate genes of insulin resistance type 2 diabetes mellitus. The aim our study was to determine the frequency single nucleotide polymorphisms (SNP) 45T>G 276G>T gene 62G>A -180C>G in patients with obesity (OB), anorexia nervosa (AN) control healthy normal-weight women (NW) influence particular genotypes on serum concentrations these hormones sensitivity. Serum adiponectin, resistin, tumor necrosis factor alpha (TNF-alpha), insulin,...
Ghrelin is a novel peptide hormone which was identified as an endogenous growth secretagogue. It mainly secreted in THE stomach, but important sites of its secretion are other parts the gastrointestinal tract. thought to be involved not only regulation also food intake and nutritional status. This study aimed investigate changes plasma ghrelin levels patients with short bowel syndrome. Twenty-four malnutrition due syndrome eleven healthy controls were included study. They underwent clinical...