A5087150935- Thyroid Disorders and Treatments
- Pregnancy and preeclampsia studies
- Thyroid Cancer Diagnosis and Treatment
- Growth Hormone and Insulin-like Growth Factors
- Adrenal Hormones and Disorders
- Vitamin D Research Studies
- Hormonal and reproductive studies
- Complement system in diseases
- BRCA gene mutations in cancer
- Prenatal Screening and Diagnostics
- Hormonal Regulation and Hypertension
- Gestational Diabetes Research and Management
- Hypothalamic control of reproductive hormones
- Genetic factors in colorectal cancer
- Glycosylation and Glycoproteins Research
- Neuroendocrine Tumor Research Advances
- Pharmacological Effects and Assays
- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Antibiotics Pharmacokinetics and Efficacy
- Sepsis Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Risks and Factors
- Gastric Cancer Management and Outcomes
- Cancer Genomics and Diagnostics
Charles University
2013-2024
General University Hospital in Prague
2012-2024
Health First
2006
Queen's University
1979
Background Insufficient function of the thyroid gland can cause low concentrations hormones which are necessary for proper development foetus brain, especially during beginning pregnancy. We determined reference interval TSH in first trimester pregnancy and decision point anti-TPO antibodies' positivity. Methods tested 5520 women from central part Czech Republic serum TSH, FT4 by chemiluminometric immunoanalysis on an ADVIA Centaur system (Siemens). The was using log transformation, then...
The guidelines of American Thyroid Association from 2011 include age over 30 as one the risk factors for hypothyroidism in pregnancy.Our objective was to verify whether increases autoimmune thyroid disease pregnancy.We performed a cross-sectional study 2006-2008 with laboratory assessment single center using primary care gynecological ambulances cooperation referral center.The included 5223 consecutive pregnant women gestational wk 9-12.We assessed occurrence pathological serum...
The aim of the study was to verify differences between reference intervals for thyroid-stimulating hormone (TSH) and free thyroxine (FT4), as well cut-off anti-thyroid peroxidase antibodies (TPO Ab) in pregnant women. Additionally, compare these with those recommended by manufacturers a healthy population. Levels TSH, FT4, TPO Ab were determined group women (n = 216) no history thyroid dysfunction. Simultaneously, function tests established 7 different analytical systems representing...
Toxoplasmosis, one of the most common zoonotic diseases worldwide, can induce various hormonal and behavioural alterations in infected hosts, its form, latent toxoplasmosis, influences course pregnancy. Autoimmune thyroid (AITD) belong to well-defined risk factors for adverse pregnancy outcomes. The aim this study was investigate whether there is a link between toxoplasmosis maternal AITD pregnancy.Cross-sectional 1248 consecutive pregnant women 9-12th gestational weeks. Serum...
Germline CHEK2 pathogenic variants confer an increased risk of female breast cancer (FBC). Here we describe a recurrent germline intronic variant c.1009-118_1009-87delinsC, which showed splice acceptor shift in RNA analysis, introducing premature stop codon (p.Tyr337PhefsTer37). The was found 21/10,204 (0.21%) Czech FBC patients compared to 1/3250 (0.03%) controls (p = 0.04) and 4/3639 (0.11%) from independent German dataset. In addition, this 5/2966 (0.17%) (but none the 443 German) ovarian...
Adiponectin is an adipocyte-derived hormone involved in the regulation of carbohydrate and lipid metabolism. Its concentrations are decreased patients with obesity, type 2 diabetes atherosclerosis higher females than males. Gender differences adiponectin levels raise possibility that sex hormones directly regulate its serum concentrations, which may turn influence insulin sensitivity different phases menstrual cycle. To test this hypothesis we measured adiponectin, estradiol, progesterone,...
The polygenic risk score (PRS) allows the quantification of effect many low-penetrance alleles on breast cancer (BC). This study aimed to evaluate performance two sets comprising 77 or 313 loci (PRS77 and PRS313) in patients with BC Czech population.
Monoallelic germline pathogenic variants (GPVs) in five Fanconi anemia (FA) genes (BRCA1/FANCS, BRCA2/FANCD1, PALB2/FANCN, BRIP1/FANCJ, and RAD51C/FANCO) confer an increased risk of breast (BC) and/or ovarian (OC) cancer, but the role GPVs 17 other FA remains unclear.
<b><i>Introduction:</i></b> Patients with ADTKD-<i>MUC1</i> have one allele producing normal mucin-1 (MUC1) and mutant MUC1, which remains intracellular. We hypothesized that patients, who only 1 secretory-competent wild-type <i>MUC1</i> allele, should exhibit decreased plasma levels. To test this hypothesis, we repurposed the serum CA15-3 assay used to measure MUC1 in breast cancer levels ADTKD-<i>MUC1</i>....
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Abstract It is controversial whether screening program for thyroid disorders in pregnancy should be universal or targeted case-finding. To evaluate the relationship between history, laboratory parameters and ultrasound (TUS) pregnant women positive screening. The included investigation of serum TSH (thyroid stimulating hormone) TPOAb (antithyroperoxidase antibodies) 5 520 unselected 9–11th gestational week. In 822 was positive: abnormal (> 3.67 < 0.06 mIU/l) and/or 143 kIU/l). 200...
Criteria for the evaluation of insulin tolerance test (ITT) and Synacthen are still a matter debate. The objective study was to make comparison serum salivary cortisol during four stimulation tests. Sixty healthy volunteers underwent ITT, with 1 (LDST), 10 (MDST) 250 (HDST) μg dose ACTH. Maximum response observed at 90 min ITT (49 %), HDST (89 %) MDST (56 40 LDST (44 %). Results expressed as 95 % confidence intervals: 408.0-843.6 289.5-868.1 nmol/l in IIT 60 min. In reached maximum...
Numerous diagnostic tests are used to evaluate the hypothalamic-pituitary-adrenal axis (HPA axis). The gold standard is still considered insulin tolerance test (ITT), but this has many limitations. Current guidelines therefore recommend Synacthen first when an HPA insufficiency suspected. However, dose of that diagnostically most accurate and sensitive a matter debate. We investigated 15 healthy men with mean/median age 27.4/26 (SD ±4.8) years, BMI (body mass index) 25.38/24.82 ±3.2) kg/m 2...
The diagnostic and prognostic role of thyroid ultrasound (TUS) in pregnant women positive for antibodies to thyroperoxidase (TPOAb) is unclear. aim our study was compare the relation texture laboratory tests controls. Using a semi-quantitative assessment we compared TUS two groups with TPOAb and/or dysfunction (TSH out 0.06-3.67 mIU/L): 186 1st trimester pregnancy recruited from universal screening 67 asymptomatic age-comparable non-pregnant non-postpartum general population (controls)....
The aim of the current research was to develop a simple and rapid mass spectrometry-based assay for determination 15 steroid hormones in human plasma single run, which would be suitable routine practice setting. For this purpose, we designed procedure based on 2D-liquid chromatography-tandem spectrometry with minimalistic sample pre-treatment. In our arrangement, preparation one takes only 10 min can accommodate 40 samples per hour when tested series. following analytical run is 18 long all...
A rat model of early sepsis induced by lipopolysaccharide (LPS) combined with interleukin-2 (IL-2) was developed. The primary aim to assess the pharmacokinetics gentamicin and sepsis-induced pathophysiological changes. Moreover, effects on glomerular filtration rate tubular function were studied in septic control rats. First, an intravenous (i.v.) bolus LPSIL-2 (1 mg/kg-Pseudomonas aeruginosa, 15 µg/kg IL-2) or saline (controls, C) administred. Wistar rats treated 30 min after as a 3 mg/kg...
Functional deficiency of mannan-binding lectin (MBL) has been associated with adverse pregnancy outcome. Adverse events during have also described in women autoimmune thyroid diseases (AITD), and hormones shown to influence serum levels MBL. Therefore, the aim this study was analyse impact MBL-deficiency on outcome relation presence AITD. Almost one year after delivery, we assessed MBL MBL2-genotypes 212 positively screened for AITD pregnancy. In 103 these women, could measure frozen samples...