A5026092123- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Prenatal Screening and Diagnostics
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer-related molecular mechanisms research
- Lipid metabolism and disorders
- Circular RNAs in diseases
- Bladder and Urothelial Cancer Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Parvovirus B19 Infection Studies
- Corneal surgery and disorders
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- Bone health and osteoporosis research
- Molecular Biology Techniques and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Renal Transplantation Outcomes and Treatments
- Drug-Induced Hepatotoxicity and Protection
- Forensic and Genetic Research
- Blood groups and transfusion
- Corneal Surgery and Treatments
- Genetic Associations and Epidemiology
- Cancer, Hypoxia, and Metabolism
Charles University
2014-2023
General University Hospital in Prague
2013-2023
Third Way
2017
Institute of Medical Biology
2014
Health First
2011
Czech Academy of Sciences
2003
Czech Academy of Sciences, Institute of Physiology
2003
Battelle
2003
Iscare I.V.F
1995
Urinary bladder carcinoma contributes to 4% of newly diagnosed oncological diseases in the Czech Republic.Biomarkers for its early non-invasive detection are therefore highly desirable.Urine seems be an ideal source such biomarkers due content cell-free nucleic acids, especially microRNAs (miRNAs).To find potential among miRNAs urine supernatant, we examined total 109 individuals (36 controls and 73 cancer patients) three phases.In first -discovery -phase, microarray cards with 381 were used...
Abstract Context: Low-grade inflammation links obesity, type 2 diabetes mellitus (T2DM), and cardiovascular diseases. Objective: To explore the expression profile of genes involved in inflammatory pathways adipose tissue peripheral monocytes (PM) obese patients with without T2DM at baseline after dietary intervention. Design: Two-week intervention study very-low-calorie diet (VLCD). Setting: University hospital. Patients: Twelve females T2DM, 8 nondiabetic (OB) 15 healthy age-matched...
Concentration of urinary cell-free DNA (ucfDNA) belongs to potential bladder cancer markers, but the reported results are inconsistent due use various non-standardised methodologies. The aim study was standardise methodology for ucfDNA quantification as a non-invasive tumour biomarker.In total, 66 patients and 34 controls were enrolled into study. Volumes each urine portion (V) recorded concentrations (c) measured using real-time PCR. Total amounts (TA) calculated compared between controls....
We studied the effect of peroxisome proliferator-activated receptor-α (PPAR-α) activation on serum concentrations and tissue expression resistin, adiponectin, adiponectin receptor-1 -2 (AdipoR1 AdipoR2) mRNA in normal mice with insulin resistance induced by lipogenic, simple-carbohydrate diet (LD). Sixteen weeks LD feeding obesity liver steatosis increased levels but did not significantly affect circulating or resistin. Treatment PPAR-α agonist fenofibrate decreased body weight fat pad...
The mechanisms of clearance circulating plasma DNA are not fully understood, and so we aimed to examine it in patients with impaired renal function compared healthy individuals. We also assessed the effect peritoneal dialysis hemodialysis on cell‐free (cfDNA) our treated patients. Overall, 20 volunteers, chronic kidney disease (CKD), 18 undergoing (PD), 17 (HD; high‐flux polysulfone membrane) were examined. Cell‐free levels determined using real‐time GADPH gene sequence amplification. cfDNA...
Insertion-deletion polymorphisms (INDELs) are diallelic markers derived from a single mutation event. Their low frequency makes them suitable for forensic and parentage testing. The examination of INDELs thus combines advantages both short tandem repeats (STR) nucleotide (SNP). This type may be examined using as small amplicon size SNP (about 100 bp) but could analyzed by techniques used routine STR analysis. For our population study, we genotyped 55 unrelated Czech individuals. We also 11...
DNA samples of 523 unrelated anonymized individuals (307 males and 216 females) born living in the Czech Republic were genotyped using Investigator® Argus X-12 system following loci localized four linkage groups: DXS10148, DXS10135, DXS8378, DXS7132, DXS10079, DXS10074, DXS10103, HPRTB, DXS10101, DXS10146, DXS10134, DXS742. Haplotype frequencies calculated for each LG (Linkage Group). The frequency most common haplotype was 0.016, 0.036, 0.042, 0.023 LG1, LG2, LG3, LG4, respectively....
The aim of the study was to define specific genetic profile in Ta and T1 urinary bladder carcinoma patients with without recurrence by gene expression microarrays.Eleven time shorter than one year (patients recurrence) 11 longer 4 years were enrolled.Data from microarrays subjected a panel statistical analyses identify cancer recurrence-associated signatures.Initial screening using GeneSpring Bioconductor software tools revealed putative set 47 genes differing both groups.After validation,...
Posterior polymorphous corneal dystrophy 3 (PPCD3) is a rare autosomal dominant disorder caused by mutations in ZEB1. To date all identified disease-causing variants were unique to the studied families, except for c.1576dup. We have detected six novel ZEB1 mutations; c.1749_1750del; p.(Pro584*) and c.1717_1718del; p.(Val573Phefs*12) two Czech c.1176dup; p.(Ala393Serfs*19), c.1100C>A; p.(Ser367*), c.627del; p.(Phe209Leufs*11) three British families splice site mutation, c.685–2A>G, patient of...
Detection and characterization of circulating cell-free fetal DNA (cffDNA) from maternal circulation requires an extremely sensitive precise method due to very low cffDNA concentration. In our study, droplet digital PCR (ddPCR) was implemented for RHD genotyping plasma compare this new quantification alternative with real-time (qPCR) as a golden standard quantitative analysis cffDNA. the first stage used. Clinical samples, including 10 non-pregnant 35 pregnant women, were analyzed next step....
Oxidative stress and apoptosis are proposed mechanisms of cellular injury in studies xenobiotic hepatotoxicity. This study is focused on addressing the mutual relationship early signals these D-galactosamine lipopolysaccharide (D-GalN/LPS) hepatotoxicity model, with help standard liver function biochemistry tests, histology, measurement gene expression by RT-PCR. Intraperitoneal injection 400 mg/kg D-GalN 50 μg/kg LPS was able to induce rats, as evidenced significant increases enzymes (ALT,...
To analyze methodological influences and characterize the concentrations of cell-free fetal DNA (cffDNA) circulating in maternal plasma at different gestational ages physiological pregnancies.We investigated 238 independent samples from single male-bearing pregnancies gestation age. In other 50 pregnancies, were collected three times during pregnancy (at all trimesters) to evaluate kinetics cffDNA. The manual automated extraction methods (Roche) compared. cffDNA was amplified using real-time...
Objective Molecular pathogenesis of Down syndrome (DS) is still incompletely understood. Epigenetic mechanisms, including miRNAs gene expression regulation, belong to potential influencing factors. The aims this study were compare expressions in placentas with normal and trisomic karyotype associate differentially expressed concrete biological pathways. Methods A total 80 CVS samples – 41 trisomy 21 39 included our study. Results obtained the pilot using real-time PCR technology TaqMan Human...