A5035548711- Bladder and Urothelial Cancer Treatments
- Urological Disorders and Treatments
- Urinary Bladder and Prostate Research
- Prostate Cancer Diagnosis and Treatment
- Urinary and Genital Oncology Studies
- Ureteral procedures and complications
- Renal cell carcinoma treatment
- Prostate Cancer Treatment and Research
- Pediatric Urology and Nephrology Studies
- Urinary Tract Infections Management
- Pelvic floor disorders treatments
- Urologic and reproductive health conditions
- Renal and Vascular Pathologies
- Renal and related cancers
- Education, Psychology, and Social Research
- Adrenal and Paraganglionic Tumors
- Cancer, Lipids, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Multiple and Secondary Primary Cancers
- Anorectal Disease Treatments and Outcomes
- Systemic Sclerosis and Related Diseases
- Cardiac tumors and thrombi
- Cancer Genomics and Diagnostics
Charles University
2011-2023
General University Hospital in Prague
2002-2023
University Hospital in Motol
2016
Urinary bladder carcinoma contributes to 4% of newly diagnosed oncological diseases in the Czech Republic.Biomarkers for its early non-invasive detection are therefore highly desirable.Urine seems be an ideal source such biomarkers due content cell-free nucleic acids, especially microRNAs (miRNAs).To find potential among miRNAs urine supernatant, we examined total 109 individuals (36 controls and 73 cancer patients) three phases.In first -discovery -phase, microarray cards with 381 were used...
<b><i>Objectives:</i></b> To determine the combination of urinary protein markers for noninvasive detection primary and recurrent urothelial bladder carcinomas. <b><i>Methods:</i></b> Urinary concentrations 27 biomarkers (NSE, ATT, AFABP, Resistin, Midkine, Clusterin, Uromodulin, ZAG2, HSP27, HSP 60, NCAM1/CD56, Angiogenin, Calreticulin, Chromogranin A, CEACAM1, CXCL1, IL13Ra2, Progranulin, VEGFA, CarbAnhydIX, Annexin-V, TIM4, Galectin1,...
Concentration of urinary cell-free DNA (ucfDNA) belongs to potential bladder cancer markers, but the reported results are inconsistent due use various non-standardised methodologies. The aim study was standardise methodology for ucfDNA quantification as a non-invasive tumour biomarker.In total, 66 patients and 34 controls were enrolled into study. Volumes each urine portion (V) recorded concentrations (c) measured using real-time PCR. Total amounts (TA) calculated compared between controls....
Tissue inhibitor of metalloproteinases 1 (TIMP-1) and matrix metalloproteinase 7 (MMP-7) were reported to have potent growth promoting activity. Lack balance between MMPs TIMPs is an important factor in the development gastrointestinal malignancies.We collected serum samples from 97 patients with metastatic colorectal cancer 79 healthy controls. Serum levels TIMP-1 MMP-7 measured immunochemically compared standard tumor markers carcinoembryonic antigen CA19-9.Serum significantly higher...
BACKGROUND: GDF-15 is a protein belonging to the transforming growth factor beta superfamily that has role in regulating inflammatory and apoptotic pathways. High level tumor tissues plasma correlate with an increased risk of recurrence reduced overall survival. OBJECTIVE: The im this study was screen capacity detecting metastatic CRC compare it standard markers CEA CA19-9. METHODS: We collected serum samples from 97 patients colorectal cancer 79 healthy controls. Serum levels GDF-15, CA19-9...
Colorectal cancer (CRC) is the second leading tumor diagnosis in women and men Czech Republic. Patient outcome depends on stage at time of and, metastatic disease, localization extent distant metastases. The early detection liver disease an important indication for surgery. Therefore, novel biomarkers are urgently required. Serum samples were collected from 97 patients with histologically confirmed CRC or progression during palliative treatment, 79 healthy controls. All exhibited adequate...
Pygeum africanum extract is available as Tadenan in many countries, including those central and eastern Europe, for the treatment of mild to moderate BPH. Its efficacy acceptability have been demonstrated numerous open placebo-controlled studies large populations. The present three-centre safety study was conducted according common protocol at urology clinics Czech Slovak Republics Poland, order confirm therapeutic profile conditions daily practice, using International Prostate Symptom Score...
Objective: To evaluate the prognostic value of depth lamina propria invasion in patients with T1 bladder cancer. Subjects and Methods: 200 were treated between years 2002 2009. Tumours above muscularis mucosae level categorised as pT1a those up to or beyond pT1b. Results: Categorisation for pT1b was performed 176 (88%). In 10 a muscle-invasive tumour found re-transurethral resection samples. 131 (79%) 166 analysed had 35 (21%) tumour. During follow-up, 101 (61%) recurred 27 (16.3%)...
Trefoil factor family (TFF) is composed of three secretory proteins (TFF1, TFF2 and TFF3) that play an important role in mucosal protection gastrointestinal tract. Their overexpression colorectal tumors seems to be associated with more aggressive disease. We collected serum samples from 79 healthy controls 97 patients metastatic cancer at the time diagnosis or progression. Serum levels TTF1-3, CEA CA19-9 were measured by ELISA. TFF1 TFF3 significantly higher compared (p < 0.0001). Moreover,...
<b><i>Objective:</i></b> To determine a predictive model for the primary diagnosis of prostate cancer (PC) based on multiple serum biomarker assay. <b><i>Material and Methods:</i></b> Between August 2011 February 2013, total 387 biopsies were performed. Serum or plasma concentrations 22 biomarkers (neopterin, IGF-1, IGFBP-2, IGFBP-3, sarcosine, endoglin, TGF-β1, periostin, sPLA2-IIa, chromogranin A, ZAG2, clusterin, PSP94, PSP94bp, leptin,...
Aim of the study is to define diagnostic accuracy selected urinary protein biomarkers in non-invasive detection primary and recurrent urothelial carcinoma bladder.The levels calprotectin, CD147, APOA4 deglycase DJ-1 were examined 255 individuals, including 60 controls with non-malignant urological disease, 61 patients a history bladder cancer negative cytology cystoscopy 134 cancer.Urinary concentrations determined by Enzyme-Linked Immunosorbent Assay (ELISA).During follow-up non-muscle...
Podoplanin, D2-40, has been described in a variety of normal and neoplastic tissues. It is often used for highlighting lymphatics. We evaluated the expression podoplanin α-smooth muscle actinpositive myofibroblasts producing suburothelial layer tunica propria urinary bladder that have some similar features with telocytes. Our results showed these cells demonstrate distinct D2-40 immunoreactivity from telocytes occurring renal pelvis ureter. observed positive reaction not only bioptic...
Aim: To assess the prognostic importance of serum levels retinol, retinol-binding protein 4 (RBP4) and vitamin E at time diagnosis in patients with renal cell carcinoma (RCC). Patients Methods: In this prospective study, a cohort 102 patients, relationships between aforementioned markers recurrence-free survival (RFS), overall (OS), as well cancer-specific (CSS), were evaluated. The A determined by high-performance liquid chromatography (HPLC), while RBP4 level enzyme-linked immunosorbent...
To identify non-invasive biomarkers of non-metastatic pancreatic cancer (PC), the blood from 186 patients (PC n=28; DM-diabetes mellitus n=60; ChP-chronic pancreatitis n=47; healthy controls n=51) was analyzed for 58 candidate biomarkers. Their effectiveness to PC compared with CA19-9. Panel defined by Random-forest (RF) analysis (CA19-9, AAT, IGFBP2, albumin, ALP, Reg3A, HSP27) outperforms CA19-9 in discrimination DM (AUC 0.92 vs. 0.82). (S100A11, CA72-4, CA19-9, CB, MMP-7, S100P-s, Reg3A)...