A5073579582- Prenatal Screening and Diagnostics
- Molecular Biology Techniques and Applications
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- MicroRNA in disease regulation
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Forensic and Genetic Research
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Renal cell carcinoma treatment
- Bladder and Urothelial Cancer Treatments
- Telomeres, Telomerase, and Senescence
- Parvovirus B19 Infection Studies
- Blood groups and transfusion
- Renal Transplantation Outcomes and Treatments
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Genomics and Phylogenetic Studies
- Cytomegalovirus and herpesvirus research
- Pregnancy and preeclampsia studies
- Extracellular vesicles in disease
- Dialysis and Renal Disease Management
- Salivary Gland Tumors Diagnosis and Treatment
Charles University
2014-2024
General University Hospital in Prague
2013-2024
University of Trnava
2022-2024
Lesy České republiky
2023
Jan Evangelista Purkyně University in Ústí nad Labem
2020
Institute of Medical Biology
2014
Weatherford College
2012
High-grade transformation of acinic cell carcinoma (AciCC) (previously referred to as dedifferentiation) is a rare phenomenon characterized by histologic progression low-grade AciCC high-grade adenocarcinoma or undifferentiated carcinoma. We report 9 new cases with immunohistochemical analysis and examination HER-2/neu p53 genes further define the profile this tumor. Histologically, component was composed polymorphic cells high mitotic rate arranged in glandular solid growth patterns...
Urinary bladder carcinoma contributes to 4% of newly diagnosed oncological diseases in the Czech Republic.Biomarkers for its early non-invasive detection are therefore highly desirable.Urine seems be an ideal source such biomarkers due content cell-free nucleic acids, especially microRNAs (miRNAs).To find potential among miRNAs urine supernatant, we examined total 109 individuals (36 controls and 73 cancer patients) three phases.In first -discovery -phase, microarray cards with 381 were used...
Abstract The cell-free DNA (cfDNA) is always present in plasma, and it biomarker of growing interest prenatal diagnostics as well oncology transplantology for therapy efficiency monitoring. But does this cfDNA have a physiological role? Here we show that presence clearance plasma healthy individuals plays an indispensable role immune system regulation. We exposed THP1 cells to individuals’ with (NP) without (TP) cfDNA. In treated NP, found elevated expression genes whose products maintain...
Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations abundance of telomeric sequences plasma and serum young healthy volunteers ability cfDNA contained these samples to co-activate TNF-α m RNA expression monocytes. performed qPCR determine relative telomere length (T/S ratios) plasma, whole blood 36 volunteers. Using paired DNase treatment, we analysed contribution co-activation mRNA THP1 monocytic cell line. found significant differences...
Concentration of urinary cell-free DNA (ucfDNA) belongs to potential bladder cancer markers, but the reported results are inconsistent due use various non-standardised methodologies. The aim study was standardise methodology for ucfDNA quantification as a non-invasive tumour biomarker.In total, 66 patients and 34 controls were enrolled into study. Volumes each urine portion (V) recorded concentrations (c) measured using real-time PCR. Total amounts (TA) calculated compared between controls....
We developed an image-to-answer algorithm available for chip-based dPCR systems with single fluorescence image capture and known image-related error removal. It automatically corrects the chip structure damage non-uniformity of light intensity.
Abstract This study elaborates on the design, fabrication, and data analysis details of SPEED, a recently proposed smartphone-based digital polymerase chain reaction (dPCR) device. The dPCR chips incorporate partition diameters ranging from 50 μm to 5 μm, these partitions are organized into six distinct blocks facilitate image processing. Due superior thermal conductivity Si its potential for mass production, were fabricated substrate. A temperature control system based high-power density...
The mechanisms of clearance circulating plasma DNA are not fully understood, and so we aimed to examine it in patients with impaired renal function compared healthy individuals. We also assessed the effect peritoneal dialysis hemodialysis on cell‐free (cfDNA) our treated patients. Overall, 20 volunteers, chronic kidney disease (CKD), 18 undergoing (PD), 17 (HD; high‐flux polysulfone membrane) were examined. Cell‐free levels determined using real‐time GADPH gene sequence amplification. cfDNA...
Insertion-deletion polymorphisms (INDELs) are diallelic markers derived from a single mutation event. Their low frequency makes them suitable for forensic and parentage testing. The examination of INDELs thus combines advantages both short tandem repeats (STR) nucleotide (SNP). This type may be examined using as small amplicon size SNP (about 100 bp) but could analyzed by techniques used routine STR analysis. For our population study, we genotyped 55 unrelated Czech individuals. We also 11...
Real-time (quantitative) polymerase chain reaction (qPCR) has been widely applied in molecular diagnostics due to its immense sensitivity and specificity. qPCR multiplexing, based either on fluorescent probes or intercalating dyes, greatly expanded PCR capability the concurrent amplification of several deoxyribonucleic acid sequences. However, probe-based multiplexing requires multiple channels, while dye-based needs primers be designed for amplicons having different melting temperatures....
A microfluidic-based digital polymerase chain reaction (dPCR) chip requires precise temperature control as well uniform distribution to ensure PCR efficiency. However, measuring local and its over thousands of μL/nL-volume samples with minimum disturbance is challenging. Here, we present a method non-contact localized measurement for determination the non-uniformity dPCR chip. We filled solution containing amplified DNA fragments known melting (TM). then captured fluorescent images when it...
Abstract Background Whole exome sequencing (WES) and whole genome (WGS) have become standard methods in human clinical diagnostics as well population genomics (POPGEN). Blood-derived genomic DNA (gDNA) is routinely used the environment. Conversely, many POPGEN studies commercial tests benefit from easy saliva sampling. Here, we evaluated quality of variant call sets level genotype concordance single nucleotide variants (SNVs) small insertions deletions (indels) for WES WGS using paired...
Autosomal dominant polycystic kidney disease (ADPKD) is the most common form of inherited that results in renal failure. ADPKD a systemic disorder with cysts and connective tissue abnormalities involving many organs. caused by mutations PKD1 gene significantly more severe than cases PKD2 mutations. The large intra-familial variability highlights role for genetic background. Here we report case family initially appearing unlinked to or loci influence mosaicism hypomorphic allele on clinical...
Detection and characterization of circulating cell-free fetal DNA (cffDNA) from maternal circulation requires an extremely sensitive precise method due to very low cffDNA concentration. In our study, droplet digital PCR (ddPCR) was implemented for RHD genotyping plasma compare this new quantification alternative with real-time (qPCR) as a golden standard quantitative analysis cffDNA. the first stage used. Clinical samples, including 10 non-pregnant 35 pregnant women, were analyzed next step....
Oncocytic papillary carcinoma with lymphoid stroma (Warthin‐like tumour) of the thyroid: a distinct entity favourable prognosis Aims : We report clinicopathological and immunohistochemical characteristics 12 cases recently recognized entity, oncocytic thyroid (PC) tumour). Methods results The were retrieved from surgical pathology files our departments. There 11 female patients one male patient; they ranged in age 45 to 85 years (mean 64.2 years). profile demonstrated positivity tumour cells...