A robust and scalable technology to fabricate ordered gold nanoparticle arrangements on epoxy substrates is presented. The nanoparticles are synthesized by solid-state dewetting nanobowled aluminum templates, which prepared the selective chemical etching of porous anodic alumina (PAA) grown an sheet with controlled oxidation. This flexible fabrication provides proper control over size, shape, interparticle distance a large surface area (several cm2), enables fine-tuning optimization their...

This study investigates various microfluidic chip fabrication techniques, highlighting their applicability and limitations in the context of urgent diagnostic needs showcased by COVID-19 pandemic. Through a detailed examination methods such as computer numerical control milling polymethyl methacrylate, soft lithography for polydimethylsiloxane-based devices, xurography glass-glass chips, micromachining-based silicon-glass we analyze each technique's strengths trade-offs. Hence, discuss...

Abstract This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The integrates the extraction purification of nucleic acids, followed by amplification via either reverse transcription-quantitative polymerase chain reaction (RT–qPCR) or transcription loop-mediated isothermal (RT–LAMP). By meeting diverse diagnostic reagent needs, platform yields testing results that closely align with those commercial RT-LAMP RT‒qPCR systems. Notable advantages...

We report on experimental observation of unidirectional propagation zero-momentum magnons in synthetic antiferromagnet consisting strained CoFeB/Ru/CoFeB trilayer. Inherent non-reciprocity spin waves antiferromagnets with uniaxial anisotropy results smooth and monotonous dispersion relation around Gamma point, where the direction phase velocity is reversed, while group conserved. The this phenomenon by intensity-, phase-, time-resolved Brillouin light scattering microscopy corroborated...

We developed an image-to-answer algorithm available for chip-based dPCR systems with single fluorescence image capture and known image-related error removal. It automatically corrects the chip structure damage non-uniformity of light intensity.

A microfluidic-based digital polymerase chain reaction (dPCR) chip requires precise temperature control as well uniform distribution to ensure PCR efficiency. However, measuring local and its over thousands of μL/nL-volume samples with minimum disturbance is challenging. Here, we present a method non-contact localized measurement for determination the non-uniformity dPCR chip. We filled solution containing amplified DNA fragments known melting (TM). then captured fluorescent images when it...

The fabrication technology of surface nanocomposites based on hexagonally ordered gold nanoparticle (AuNP) layers (quasi-arrays) and their possible application as surface-enhanced Raman spectroscopy (SERS) substrates are presented in this paper. prepared using a nanotextured template formed by porous anodic alumina (PAA) combined with thin-film deposition subsequent solid-state dewetting. Three types hexagonal arrangements were different D/D0 values (where D is the interparticle gap, D0...

The fabrication and characterization of a localized surface plasmon resonance (LSPR) based optical nanosensor, which utilizes highly ordered gold nanoparticles as transducers are presented in this work. synthetized using nanodimpled aluminium templates, were prepared by selective chemical etching the porous anodic alumina grown over an sheet. formed directly transferred to PDMS (polydimethylsiloxane) microfluidic cells, where their LSPR transmittance spectra measured. effect particle size...

Localized surface plasmon resonance (LSPR) gas sensitivity is introduced as a new parameter to evaluate the performance of plasmonic sensors. A model proposed consider sensors' and decay length correlate LSPR response, measured upon exchange, with an equivalent refractive index change consistent adsorbed layers. To demonstrate applicability this parameter, ellipsoidal gold nanoparticles (NPs) arranged in densely packed hexagonal lattices were fabricated. The main advantages these sensors are...

The applicability of a novel gold–epoxysurface nanocomposite for surface-enhanced Raman scattering (SERS) is investigated. consists ellipsoidal nanoparticles in hexagonal arrangement, where the average particle diameter (D0) and interparticle gap (D) can be controlled 0.15–1.00 (D/D0) range on large surface area (several cm 2). Numerical simulations were used to estimate SERS enhancement factors substrates with five different arrangements. fabricated substrates' was functionalized 20...

Abstract Increasing the electrochemical surface area of noble metal electrodes is vital for many applications, including catalysis and bioelectronics. Herein, a method presented obtaining porous thin films via reactive magnetron sputtering oxides, MO x , followed by their reduction using chemical reducers or current. Variation conditions yields range different morphological properties. This metals rapid, facile, compatible with microfabrication processes. The resulting metallic are have...

Electrodeposition of tantalum thin coating was attempted from a room temperature ionic liquid, BMP[Tf2N] in presence dissolved TaF5 and LiF. The influence deposition parameters, such as liquid composition, substrate type, electrical potential or upon structure composition observed. Cyclic voltammetry, scanning transmission electron microscopy (STEM) conjunction with energy dispersive X-ray analysis (EDX), photoelectron spectroscopy (XPS), well diffraction (XRD) techniques were applied....

Electrodeposition of tantalum thin coating was attempted from a room temperature ionic liquid, BMP[Tf2N] in presence dissolved TaF5 and LiF. The influence deposition parameters, such as liquid composition, substrate type, electrical potential or upon structure composition observed. Cyclic voltammetry, scanning transmission electron microscopy (STEM) conjunction with energy dispersive X-ray analysis (EDX), photoelectron spectroscopy (XPS), well diffraction (XRD) techniques were applied....

Gold/epoxy nanocomposites were prepared by template-assisted solid-state dewetting and tested as a Surface-Enhanced Raman Scattering (SERS) substrates for DNA detection. The effect of excitation wavelength on the detected peak intensities was investigated with three different laser sources (532, 633, 785 nm). relationship between plasmon absorbance band substrate studied. It shown that shift in band, caused binding target-DNA molecules could have major intensity measured peaks, which can be...

In this work, the effect of proper focal depth on obtained surface-enhanced Raman spectroscopy (SERS) signal a nanostructured surface has been studied. For this, ellipsoidal gold nanoparticles arranged in hexagonal array top an epoxy substrate were used. The functionalized with DNA molecules via thiol-chemistry. By using confocal microscopy setup and pinhole, spectrum sample was recorded 100 nm resolution normal (Z) direction ±15 μm range. results confirm that peaks characteristic to...

We demonstrate a smartphone integrated handheld (SPEED) digital polymerase chain reaction (dPCR) device for point-of-care application. The has dimensions of 100×200×35 mm3 and weight ≈ 400 gm can perform 45 cycles in 25 min. integrated, miniaturized modules thermal cycling, image taking, wireless data communication that are controlled by self-developed android-based applications. only consumable is the developed silicon-based dPCR chip, which potential to be recycled. device’s precision...

surface-enhanced Raman spectroscopy (SERS) is adopted in a microfluidic cell to detect Rhodamine 6G (R6G) dye molecules using gold nanoparticle Au NP’s –epoxy surface nanocomposite as substrates. We studied the effect of changing environment’s refractive index RI at nanocomposites’ by solution 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-4</sup> M different water + ethylene glycol (EG) mixtures with concentrations ranging from 25-100%....