A5075817740- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Helminth infection and control
- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Parasitic Diseases Research and Treatment
- Aquaculture disease management and microbiota
- Parasitic infections in humans and animals
- Insects and Parasite Interactions
- Vector-borne infectious diseases
- Trace Elements in Health
- Identification and Quantification in Food
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- Environmental DNA in Biodiversity Studies
- Salmonella and Campylobacter epidemiology
- Entomopathogenic Microorganisms in Pest Control
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Toxoplasma gondii Research Studies
- Microbial infections and disease research
- Insect Resistance and Genetics
- Polyamine Metabolism and Applications
- Agricultural safety and regulations
- SARS-CoV-2 detection and testing
Masaryk University
2016-2025
Institute of Applied Biotechnologies (Czechia)
2020-2024
Charles University
2009-2019
Monitoring gastrointestinal helminth infections in wild ruminants poses significant challenges for managing wildlife health, particularly regarding invasive species. Traditional coprological methods are often limited by their labor-intensive nature and potential erroneous identification due to morphological similarities among parasite This study employed advanced molecular techniques assess the prevalence distribution of several taxa, including nematode Ashworthius sidemi trematode...
Abstract Lymnaeid snails of the genus Radix serve as intermediate hosts some schistosomes and fasciolids. In Europe, delineation species within is unresolved and, therefore, spectrum snail susceptible to trematode infections under discussion. We used compared three criteria for using collected at 43 localities. (a) Sequence analysis ITS-2 rDNA disclosed that belong four — R. auricularia (Linnaeus, 1758), peregra (Müller, 1774), lagotis (Schrank, 1803) labiata (Rossmaessler, 1835) (criteria...
Lymnaea palustris and L. fuscus are members of the European stagnicolines (Gastropoda: Lymnaeidae). The role in transmission Fasciola hepatica has been often proposed. To assess possible relationship between these two F. Sweden, field monitoring parallel with experimental infections were conducted. Stagnicoline snails collected identified on pastures grazed by either sheep or cattle four farms suffering from fasciolosis Sweden during 2011-2012. Field-collected examined for DNA PCR. In...
Abstract Background Whole exome sequencing (WES) and whole genome (WGS) have become standard methods in human clinical diagnostics as well population genomics (POPGEN). Blood-derived genomic DNA (gDNA) is routinely used the environment. Conversely, many POPGEN studies commercial tests benefit from easy saliva sampling. Here, we evaluated quality of variant call sets level genotype concordance single nucleotide variants (SNVs) small insertions deletions (indels) for WES WGS using paired...
Parasitic liver flukes of the family Fasciolidae are responsible for major socioeconomic losses worldwide. However, at present, knowledge fundamental molecular biology these organisms is scant. Here, we characterize, first time, transcriptome and secreted proteome adult stage "giant fluke," Fascioloides magna, using Illumina sequencing technology one-dimensional SDS-PAGE OFFGEL protein electrophoresis, respectively. A total ∼54,000,000 reads were generated assembled into ∼39,000 contiguous...
The giant liver fluke, Fascioloides magna, is of interest to wild-life managers, veterinarians and researchers, due its unusual body size (3-10 cm), high pathogenic potential because it continuously spreading new areas, especially in Europe. Annually, the number cases animal infections (mainly cervids bovids) caused by this fluke monitored many European countries, including Czech Republic (with some foci prevalence over 90%). During years 2009 2010, 1622 survey forms focused on monitoring...
Cysteine peptidases of clan CA, family C1 account for a major part proteolytic activity in the haematophagous monogenean Eudiplozoon nipponicum. The full spectrum cysteine cathepsins is, however, unknown and their particular biochemical properties, tissue localisation, involvement parasite-host relationships are yet to be explored. Sequences L B (EnCL EnCB) were mined from E. nipponicum transcriptome analysed bioinformatically. Genes encoding two EnCLs one EnCB cloned recombinant proteins...
Parasite inhibitors of cysteine peptidases are known to influence a vast range processes linked degradation either the parasites' own proteins or native their hosts. We characterise novel type I cystatin (stefin) found in sanguinivorous fish parasite Eudiplozoon nipponicum (Platyhelminthes: Monogenea). have identified transcript its coding gene transcriptome adult worms. Its amino acid sequence is similar other stefins except for containing legumain-binding domain, which this cystatins...
Single next-generation sequencing (NGS) proved to be an important tool for monitoring the SARS-CoV-2 outbreak at global level Until today, thousands of genome sequences have been published GISAID (Global Initiative on Sharing All Influenza Data) but only a portion are suitable reliable variant analysis. Here we report comparison three commercially available NGS library preparation kits. We discuss advantages and limitations from perspective required input sample quality data advanced
Abstract Background Ectoparasites from the family Diplozoidae (Platyhelminthes, Monogenea) belong to obligate haematophagous helminths of cyprinid fish. Current knowledge these worms is for most part limited their morphological, phylogenetic, and population features. Information concerning biochemical molecular nature physiological processes involved in host–parasite interaction, such as evasion immune system its regulation, digestion macromolecules, suppression blood coagulation...
SUMMARY Cercariae of the bird schistosome Trichobilharzia regenti and human Schistosoma mansoni employ proteases to invade skin their definitive hosts. To investigate whether a similar proteolytic mechanism is used by both species, cercarial extracts T. S. were biochemically characterized, with primary focus on cysteine peptidases. A pattern peptidase activities was detected zymography chromatographic fractions from mansoni. The greatest activity recorded in species against fluorogenic...
Serine peptidases are involved in many physiological processes including digestion, haemostasis and complement cascade. Parasites regulate activities of host serine to their own benefit, employing various inhibitors, which belong the Kunitz-type protein family. In this study, we confirmed presence potential anticoagulants extracts haematophagous monogenean Eudiplozoon nipponicum parasitizes common carp. We then focused on a Kunitz (EnKT1) discovered E. transcriptome, structurally resembles...
Representatives of the trematode family Fasciolidae are responsible for major socio-economic losses worldwide. Fascioloides magna is an important pathogenic liver fluke wild and domestic ungulates. To date, only a limited number studies concerning molecular biology F. exist. Therefore, objective present study was to determine complete mitochondrial (mt) genome sequence magna, assess phylogenetic relationships this with other trematodes based on mtDNA dataset. The mt 14,047 bp. gene content...
To date, most molecular investigations of schistosomatids have focused principally on blood flukes (schistosomes) humans. Despite the clinical importance cercarial dermatitis in humans caused by Trichobilharzia regenti and serious neuropathologic disease that this parasite causes its permissive avian hosts accidental mammalian hosts, almost nothing is known about aspects how fluke invades migrates host tissues it interacts with hosts' immune system. Here, we explored selected using a...
The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of GIN community not available with this and it commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in post-treatment survey, but results provided are usually only qualitative semi-quantitative. updated WAAVP guidelines now recommend assessing anthelmintic for each...
Abstract Experimental infections of Galba sp. (origin, Colombia) with allopatric isolates Fasciola hepatica from France or Fascioloides magna the Czech Republic were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development either parasite. In snails exposed F. , 7 400 harboured several rediae and only two contained a small number free cercariae on day 50 post-exposure. contrast, intensity infection in progressively increased...
The diagnosis of gastrointestinal nematode (GIN) infections in ruminants is routinely based on morphological/morphometric analysis parasite specimens recovered by coprological methods, followed larval culture (LC) techniques. Such an approach laborious, time-consuming, requires a skilled expert, and moreover suffers from certain limitations. Molecular tools are able to overcome the majority these issues, providing accurate identification species and, therefore, may be valuable sustainable...