A5001561535- Biochemical and Molecular Research
- Metal-Catalyzed Oxygenation Mechanisms
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
- Advanced Nanomaterials in Catalysis
- Cellular transport and secretion
- Oxidative Organic Chemistry Reactions
- Microbial Natural Products and Biosynthesis
- Enzyme Structure and Function
- Adenosine and Purinergic Signaling
- Advanced Photocatalysis Techniques
- Genetics and Neurodevelopmental Disorders
- Parasites and Host Interactions
- Peroxisome Proliferator-Activated Receptors
- Calcium signaling and nucleotide metabolism
- Synthetic Organic Chemistry Methods
- Parasitic Infections and Diagnostics
- Microbial Metabolic Engineering and Bioproduction
- Protein Kinase Regulation and GTPase Signaling
- Metalloenzymes and iron-sulfur proteins
- Electrochemical sensors and biosensors
- Endoplasmic Reticulum Stress and Disease
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Synthesis and Catalytic Reactions
University of York
2012-2024
Walter Reed Army Institute of Research
2009
University of Liverpool
1996-2006
The Pirbright Institute
2002
University of Dundee
2002
University of Warsaw
2002
Substrate channeling in morphine biosynthesis Poppies are still the most economically viable source of excellent painkiller morphine. Winzer et al. have now identified a key enzyme poppy's biosynthetic pathway for The turns out to be an unusual protein that contains both cytochrome P-450 and oxidoreductase modules. Together these modules process two subsequent steps pathway. identification this may enable alternate routes less dependent on poppy cultivation. Science , issue p. 309
Abstract Controlling the selectivity of a chemical reaction with external stimuli is common in thermal processes, but rare visible‐light photocatalysis. Here we show that redox potential carbon nitride photocatalyst (CN‐OA‐m) can be tuned by changing irradiation wavelength to generate electron holes different oxidation potentials. This tuning was key realizing photo‐chemo‐enzymatic cascades give either ( S )‐ or R )‐enantiomer phenylethanol. In combination an unspecific peroxygenase from...
Aps1 from <i>Schizosaccharomyces pombe</i>(Ingram, S. W., Stratemann, A., and Barnes, L. D. (1999) <i>Biochemistry</i> 38, 3649–3655) YOR163w from<i>Saccharomyces cerevisiae</i> (Cartwright, J. L., McLennan, A. G. <i>J. Biol. Chem.</i> 274, 8604–8610) have both previously been characterized as MutT family hydrolases with high specificity for diadenosine hexa- pentaphosphates (Ap<sub>6</sub>A Ap<sub>5</sub>A). Using purified recombinant preparations of these enzymes, we now discovered that...
Abstract Having identified inconsistencies when repeating literature examples of photochemical transformations and difficulties recreating experimental setups, we devised several criteria that an ideal lab‐scale reactor should achieve. Herein, introduce a versatile photoreactor for high‐throughput screening, preparative‐scale batch reactions continuous processing, all with single light source. The utilizes interchangeable arrays pseudo‐monochromatic high‐power LEDs in range synthetically...
The PCD1 nudix hydrolase gene of Saccharomyces cerevisiae has been cloned and the Pcd1p protein characterized as a diphosphatase (pyrophosphatase) with specificity for coenzyme A CoA derivatives. Oxidized disulfide is preferred over substrate K(m) k(cat) values 24 micrometer 5.0 s(-1), respectively, compared 280 4.6 s(-1) respectively. products hydrolysis were 3'-phosphoadenosine 5'-monophosphate 4'-phosphopantetheine. F(-) ions inhibited activity an IC(50) 22 micrometer. sequence contains...
A total of 17 Nudix hydrolases were tested for their ability to hydrolyze 5-phosphoribosyl 1-pyrophosphate (PRPP). All 11 enzymes that active toward dinucleoside polyphosphates with 4 or more phosphate groups as substrates also able PRPP, whereas the 6 could not and have coenzyme A, NDP-sugars, pyridine nucleotides preferred did degrade PRPP. The products hydrolysis ribose 1,5-bisphosphate Pi. Active PRPP pyrophosphatases included diphosphoinositol polyphosphate phosphohydrolase (DIPP)...
A gene from the marine bacterium Stenotrophomonas maltophilia encodes a 38.6 kDa FAD-containing flavoprotein (Uniprot B2FLR2) named S. flavin-containing monooxygenase (SMFMO), which catalyses oxidation of thioethers and also regioselective Baeyer-Villiger model substrate bicyclo[3.2.0]hept-2-en-6-one. The enzyme was unusual in its ability to employ either NADH or NADPH as nicotinamide cofactor. K(M) k(cat) values for were 23.7±9.1 μM 0.029 s(-1) 27.3±5.3 0.022 NADPH. However, /K(M) value...
Abstract H 2 O ‐driven enzymes are of great interest for industrial biotransformations. Herein, we show the first time that oxalate oxidase (OXO) is an efficient in situ source one these biocatalysts, which known as unspecific peroxygenase (UPO). OXO reasonably robust, produces only CO a by‐product and uses cheap sacrificial electron donor. UPO has significant potential catalyst selective C−H oxyfunctionalisations, confirm herein by testing diverse drug panel using miniaturised...
Unspecific peroxygenases (UPOs) have emerged as attractive biocatalysts for selective oxygenations because, unlike cytochromes P450, they can be employed easy-to-use lyophilized powders and depend only upon hydrogen peroxide the external oxidant. The application of UPOs to a range synthetic challenges relies on characterization activity specificity complementary enzymes. Here, we show that two UPOs, artUPO rAaeUPO-PaDa-I-H, which are representative members "short" family I "long" II display...
The YOR163w open reading frame on chromosome XV of the Saccharomyces cerevisiae genome encodes a member MutT motif (nudix hydrolase) family enzymes Mr 21,443. By cloning and expressing this gene in Escherichia coli S. cerevisiae, we have shown product to be (di)adenosine polyphosphate hydrolase with previously undescribed substrate specificity. Diadenosine 5',5"'-P1, P6-hexaphosphate is preferred substrate, hydrolysis H218O shows that ADP adenosine 5'-tetraphosphate are produced by attack at...
The human homologue of the Saccharomyces cerevisiae YSA1 protein, YSA1H, has been expressed as a thioredoxin fusion protein in Escherichia coli. It is an ADP-sugar pyrophosphatase with similar activities towards ADP-ribose and ADP-mannose. Its ADP-glucose diadenosine diphosphate were 56% 20% that respectively, whereas its activity other nucleoside 5'-diphosphosugars was typically 2-10%. cADP-ribose not substrate. products hydrolysis AMP ribose 5-phosphate. K(m) k(cat) values 60 microM 5.5...
A lyophilized preparation of an unspecific peroxygenase variant from Agrocybe aegerita (rAaeUPO-PaDa-I-H) is a highly effective catalyst for the oxygenation diverse range N-heterocyclic compounds. Scalable biocatalytic oxygenations (27 preparative examples, ca. 100 mg scale) have been developed across wide substrates, including alkyl pyridines, bicyclic N-heterocycles and indoles. H
Unspecific Peroxygenases (UPOs) are increasingly significant enzymes for selective oxygenations as they stable, highly active and catalyze their reactions at the expense of only hydrogen peroxide oxidant. Their structural similarity to chloroperoxidase (CPO) means that UPOs can also halogenation based upon generation hypohalous acids from halide H
Abstract Unspecific Peroxygenases (UPOs) have emerged as robust biocatalysts for selective oxygenation reactions, they are easily produced at scale and require only hydrogen peroxide the external oxidant. UPOs can catalyze of primary benzylic carbons toluenes to give alcohol, aldehyde carboxylic acid products. They also hydroxylation position ethylbenzenes, subsequent oxidation secondary alcohols ketones. In this study, we investigated factors that affect balance products in UPO‐catalyzed...
The unspecific peroxygenase (UPO) from
The African swine fever virus (ASFV) g5R gene encodes a protein containing Nudix hydrolase motif which in terms of sequence appears most closely related to the mammalian diadenosine tetraphosphate (Ap4A) hydrolases. However, purified recombinant (g5Rp) showed much wider range nucleotide substrate specificity compared eukaryotic Ap4A hydrolases, having highest activity with GTP, followed by adenosine 5'-pentaphosphate (p5A) and dGTP. Diadenosine diguanosine nucleotides were substrates, but...
Proteasomes are molecular machines found in virtually all cells that provide one of the mechanisms for protein turnover. We have analysed 20S proteasome Schistosoma mansoni, first multimeric complex isolated from this helminth parasite. Three chromatographic steps were employed to yield a highly homogeneous preparation. 2-DE purified revealed 58 spots, which 46 could be assigned either an alpha or beta signature by MS. Most 14 transcripts (7alpha and 7beta) encoded parasite genome...
ARF GTPases are central regulators of membrane trafficking that control local identity and remodeling facilitating vesicle formation. Unraveling their function is complicated by the overlapping association ARFs with guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), numerous interactors. Through a functional genomic screen three-dimensional (3D) prostate cancer cell behavior, we explore contribution GTPases, GEFs, GAPs, interactors to collective invasion. This...
Controlling the selectivity of a chemical reaction with external stimuli is common in thermal processes, but rare visible-light photocatalysis. Here we show that redox potential carbon nitride photocatalyst (CN-OA-m) can be tuned by changing irradiation wavelength to generate electron holes different oxidation potentials. This tuning was key realizing photo-chemo-enzymatic cascades give either (