A5053668530- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Enzyme Catalysis and Immobilization
- RNA Research and Splicing
- RNA modifications and cancer
- Microbial metabolism and enzyme function
- Microbial Metabolic Engineering and Bioproduction
- Ubiquitin and proteasome pathways
- NF-κB Signaling Pathways
- Galectins and Cancer Biology
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- RNA regulation and disease
- Signaling Pathways in Disease
- Neurological diseases and metabolism
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- interferon and immune responses
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Mycobacterium research and diagnosis
- Erythrocyte Function and Pathophysiology
- Bacterial Genetics and Biotechnology
Helmholtz Zentrum München
2016-2025
Target (Germany)
2022-2023
Target (United States)
2022
Center for Integrated Protein Science Munich
2015-2021
Technical University of Munich
2015-2021
Protein Express (United States)
2004-2017
Institute of Bioinformatics and Systems Biology
2015
Center for Environmental Health
2014
European Molecular Biology Laboratory
2003-2013
European Molecular Biology Laboratory
2006-2012
Despite the importance of superoxide dismutases (SODs) in plant antioxidant defence system little is known about their regulation by post-translational modifications. Here, we investigated vitro effects nitric oxide derivatives on seven SOD isoforms Arabidopsis thaliana. S-nitrosoglutathione, which causes S-nitrosylation cysteine residues, did not influence activities. By contrast, peroxynitrite inhibited mitochondrial manganese SOD1 (MSD1), peroxisomal copper/zinc SOD3 (CSD3), and...
Autophagy-driven nuclear transfer of miR-126-5p guided by Mex3a enables direct inhibition caspase-3 and protects against atherosclerosis.
Significance Leucine-rich repeat kinase 2 (LRRK2) is a multidomain protein implicated in Parkinson disease, and cAMP-dependent (PKA) has been suggested to act as an upstream phosphorylating LRRK2. Using phosphoproteomics approach, we identified several novel PKA phosphorylation sites on We could demonstrate that one phosphosite comprises the second most common mutation LRRK2 (R1441C/G/H) attributed disease. Our findings reveal this site mandatory for subsequent 14-3-3 binding affects...
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions size-constrained metal biomineralization. The protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian which sets native (EncB,C,D) engineered self-target enabling localized bimolecular fluorescence enzyme complementation. Encapsulation the tyrosinase leads confinement toxic...
Abstract Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) drives viral B cell transformation and oncogenesis. LMP1’s transforming activity depends on its C-terminal activation region 2 (CTAR2), which induces NF-κB JNK by engaging TNF receptor-associated factor 6 (TRAF6). The mechanism of TRAF6 recruitment to LMP1 role in signalling remains elusive. Here we demonstrate that interacts directly with a binding motif within CTAR2. Functional NMR studies supported molecular modeling...
Abstract Dystonia is a rare-disease trait for which large-scale genomic investigations are still underrepresented. Genetic heterogeneity among patients with unexplained dystonia warrants interrogation of entire genome sequences, but this has not yet been systematically evaluated. To significantly enhance our understanding the genetic contribution to dystonia, we (re)analyzed 2,874 whole-exome sequencing (WES), 564 whole-genome (WGS), as well 80 fibroblast-derived proteomics datasets,...
The stability and homogeneity of a protein sample is strongly influenced by the composition buffer that in. A quick easy approach to identify which increases possibly conformational fluorescence-based thermal-shift assay (Thermofluor). Here, novel 96-condition screen for Thermofluor experiments presented consists additive parts. comprises 23 different buffers includes small-molecule additives such as salts nucleotide analogues. utilization components increase thermal frequently results in...
Stroke induces a multiphasic systemic immune response, but the consequences of this response on atherosclerosis-a major source recurrent vascular events-have not been thoroughly investigated. We show that stroke exacerbates atheroprogression via alarmin-mediated propagation inflammation. The prototypic brain-released alarmin high-mobility group box 1 protein induced monocyte and endothelial activation receptor for advanced glycation end products (RAGE)-signaling cascade increased plaque load...
Nitric oxide (NO) has emerged as a signaling molecule in plants being involved diverse physiological processes like germination, root growth, stomata closing and response to biotic abiotic stress. S-nitrosoglutathione (GSNO) biological NO donor very important function since it can transfer its moiety other proteins (trans-nitrosylation). Such trans-nitrosylation reactions are equilibrium depend on GSNO level. The breakdown of thus the level S-nitrosylated regulated by GSNO-reductase (GSNOR)....
Histone post-translational modifications (PTMs) are key players in chromatin regulation. The identification of novel histone acylations raises important questions regarding their role transcription. In this study, we characterize the an acylation on lateral surface octamer, H3K122 succinylation (H3K122succ), function and Using succinylated at vitro transcription assays, show that presence H3K122succ is sufficient to stimulate line with this, found our ChIP assays enriched promoters active...
Article9 May 2021Open Access Source Data Interferon-induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20 Daniela Stadler orcid.org/0000-0002-7342-9751 Institute Virology, School Medicine, Technical University Munich / Helmholtz Zentrum München, Munich, Germany Search for more papers by this author Martin Kächele GermanyThese authors contributed equally to work Alisha N Jones Structural Biology, Neuherberg, Center Integrated Protein Science and Bavarian NMR at...
Polyubiquitin chains are flexible multidomain proteins, whose conformational dynamics enable them to regulate multiple biological pathways. Their dynamic is determined by the linkage between ubiquitins and number of ubiquitin units. Characterizing polyubiquitin behavior as a function their length hampered because increasing system size variability. Here, we introduce new approach efficiently integrating small-angle x-ray scattering with simulations allowing us accurately characterize linear...
Phosphopantetheine adenylyltransferase (PPAT) catalyzes the penultimate step in coenzyme A (CoA) biosynthesis: reversible adenylation of 4′-phosphopantetheine yielding 3′-dephospho-CoA and pyrophosphate. Wild-type PPAT from Escherichia coli was purified to homogeneity. N-terminal sequence analysis revealed that enzyme is encoded by a gene designated kdtB, purported encode protein involved lipopolysaccharide core biosynthesis. The gene, here renamed coaD, found wide range microorganisms,...
23 different crystal forms of 19 biological macromolecules were examined with respect to their anomalously scattering substructures using diffraction data collected at a wavelength 2.0 Å (6.2 keV). In more than 90% the cases substructure was found contain just protein S atoms. The presented suggest that chloride, sulfate, phosphate or metal ions from buffer even purification protocol are frequently bound molecule and these often overlooked, especially if they not full occupancy. Thus, in...
Multi-domain proteins play critical roles in fine-tuning essential processes cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. (RBPs) represent an important class of multi-domain proteins, which regulate expression recognizing linear structured sequence motifs. Here, we employ segmental perdeuteration the three recognition motif (RRM) RBP TIA-1 using...
Celastrol is a natural pentacyclic triterpene used in traditional Chinese medicine with significant weight-lowering effects. Celastrol-administered mice at 100 μg/kg decrease food consumption and body weight via leptin-dependent mechanism, yet its molecular targets this pathway remain elusive. Here, we demonstrate vivo that celastrol-induced loss largely mediated by the inhibition of leptin negative regulators protein tyrosine phosphatase (PTP) 1B (PTP1B) T-cell PTP (TCPTP) arcuate nucleus...
A helminth larval extract and its component Hpb glutamate dehydrogenase induce an anti-inflammatory mediator switch to suppress type-2 inflammation.