A5023396427- Acute Ischemic Stroke Management
- Traumatic Brain Injury and Neurovascular Disturbances
- Stroke Rehabilitation and Recovery
- Cerebrovascular and Carotid Artery Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Nanoparticle-Based Drug Delivery
- Barrier Structure and Function Studies
- Blood Coagulation and Thrombosis Mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- Cerebrospinal fluid and hydrocephalus
- Intracranial Aneurysms: Treatment and Complications
- S100 Proteins and Annexins
- Neurological Disease Mechanisms and Treatments
- Peripheral Artery Disease Management
- Nanoplatforms for cancer theranostics
- Characterization and Applications of Magnetic Nanoparticles
- Nerve injury and regeneration
- Inflammatory Bowel Disease
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Anesthesia and Neurotoxicity Research
Cyceron
2014-2024
Inserm
2015-2024
Université de Caen Normandie
2015-2024
Centre Hospitalier Universitaire de Caen Normandie
2013-2024
Normandie Université
2016-2024
Physiopathologie et imagerie des troubles neurologiques
2017-2024
Centre Hospitalier Universitaire de Limoges
2022
Centre Hospitalier Sainte-Anne
2021
University of Pennsylvania
2017
Direction de la Recherche Fondamentale
2017
The aim of the present study was to investigate impact different stroke subtypes on glymphatic system using MRI.We first improved and characterized an in vivo protocol measure perfusion MRI after minimally invasive injection a gadolinium chelate within cisterna magna. Then, integrity evaluated 4 models mice including subarachnoid hemorrhage (SAH), intracerebral hemorrhage, carotid ligature, embolic ischemic stroke.We were able reliably evaluate function MRI. Moreover, we provided evidence...
INTRODUCTION:According to the "glymphatic system" hypothesis, brain waste clearance is mediated by a continuous replacement of interstitial milieu bulk flow cerebrospinal fluid (CSF).Previous reports suggested that this cerebral CSF circulation only active during general anesthesia or sleep, an effect dilatation extracellular space.Given controversies regarding plausibility phenomenon and limitations currently available methods image glymphatic system, we developed original whole-brain in...
Platelet cross-linking during arterial thrombosis involves von Willebrand Factor (VWF) multimers. Therefore, proteolysis of VWF appears promising to disaggregate platelet-rich thrombi and restore vessel patency in acute thrombotic disorders such as ischemic stroke, coronary syndrome, or limb ischemia. N-Acetylcysteine (NAC, a clinically approved mucolytic drug) can reduce intrachain disulfide bonds large polymeric proteins. In the present study, we postulated that NAC might cleave multimers...
Subarachnoid hemorrhage (SAH) is a devastating form of stroke with neurological outcomes dependent on the occurrence delayed cerebral ischemia. It has been shown in rodents that some mechanisms leading to ischemia are related decreased circulation cerebrospinal fluid (CSF) within brain parenchyma. Here, we evaluated CSF nonhuman primate physiological condition and after SAH.We first Macacafacicularis, using magnetic resonance imaging temporal DOTA-Gd distribution its injection into CSF....
Hyperfibrinolysis is a systemic condition occurring in various clinical disorders such as trauma, liver cirrhosis, and leukemia. Apart from increased bleeding tendency, the pathophysiological consequences of hyperfibrinolysis remain largely unknown. Our aim was to develop an experimental model study its effects on homeostasis blood-brain barrier (BBB). We induced sustained hyperfibrinolytic state mice by hydrodynamic transfection plasmid encoding for tissue-type plasminogen activator (tPA)....
Our aim was to assess the spatiotemporal evolution of cerebrovascular inflammation occurring after ischemic and hemorrhagic strokes using a recently developed, fast, ultra-sensitive molecular MRI method.We first assessed longitudinally triggered by collagenase-induced hemorrhage permanent/transient middle cerebral artery occlusion in mice, injection microparticles iron oxide targeted vascular cell adhesion molecule-1 (MPIOs-αVCAM-1). Thereafter, we used this method study anti-inflammatory...
We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on composition protein corona and their correspondence toxicological issues.SPIONs different sizes surface charges were exposed fetal bovine serum. The structure/composition biological corona-SPION complexes probed.The affinity level adsorption specific proteins is strongly dependent size charge SPIONs. In vivo experiments mouse blood-brain barrier model revealed that nontargeted SPIONs...
Background and Purpose— In experimental models of ischemic stroke, abrupt reperfusion is associated with secondary brain damages, responsible for up to 70% the final lesion size. Whether this remains true in humans unknown. Methods— Using data from ASTER randomized trial (Aspiration vs Stent Retriever Successful Revascularization), we investigated effect complete (defined as a modified Thrombolysis Cerebral Infarction 3) after endovascular thrombectomy on early growth assessed by...
Stroke induces a multiphasic systemic immune response, but the consequences of this response on atherosclerosis-a major source recurrent vascular events-have not been thoroughly investigated. We show that stroke exacerbates atheroprogression via alarmin-mediated propagation inflammation. The prototypic brain-released alarmin high-mobility group box 1 protein induced monocyte and endothelial activation receptor for advanced glycation end products (RAGE)-signaling cascade increased plaque load...
Neurointerventionists lack guidelines for the use of antithrombotic therapies in their clinical practice; consequently, there is likely to be significant heterogeneity between centers. Through a nationwide survey, we aimed obtain an exhaustive cross-sectional overview neurointerventional procedures France.In April 2021, French surgery centers were invited participate 51-question survey disseminated through active trainee-led research collaborative network (the JENI-RC).All 40 answered...
Tissue plasminogen activator (tPA) is the only available treatment for acute stroke. In addition to its vascular fibrinolytic action, tPA exerts various effects within brain, ranging from synaptic plasticity control of cell fate. To date, influence in ischemic brain has been investigated on neuronal, microglial, and endothelial We addressed mechanism action oligodendrocyte (OL) survival extent white matter lesions also impact aging these processes. observed that, parallel reduced levels OLs,...
Tissue-type plasminogen activator (tPA) is the only drug approved for acute treatment of ischemic stroke but with two faces in disease: beneficial fibrinolysis vasculature and damaging effects on neurovascular unit brain parenchyma. To improve this profile, we developed a novel strategy, relying antibodies targeting proneurotoxic tPA.After production characterization (αATD-NR1) that specifically prevent interaction tPA ATD-NR1 N-methyl-d-aspartate receptors, have evaluated their efficacy...
Vesicular (v)- and target (t)-SNARE proteins assemble in SNARE complex to mediate membrane fusion. Tetanus neurotoxin-insensitive vesicular-associated protein (TI-VAMP/VAMP7), a vesicular expressed several cell types including neurons, was previously shown play major role exocytosis involved neurite growth cultured neurons. Here we generated complete constitutive knock-out by deleting the exon 3 of Vamp7 . Loss TI-VAMP expression did not lead any striking developmental or neurological...
In the field of ischemic cerebral injury, precise characterization neurovascular hemodynamic is required to select candidates for reperfusion treatments.It thus admitted that advanced imaging-based approaches would be able better diagnose and prognose those patients contribute clinical care.Current imaging modalities like MRI allow a diagnostic injury but suffer from limited availability transportability.The recently developed ultrafast ultrasound could powerful tool perform emergency long...
Procedural complications in thrombectomy for large vessel occlusions of the anterior circulation are not well described. We investigated incidence, risk factors, and clinical implications daily practice.We used data from ongoing prospective multicenter observational Endovascular Treatment Ischemic Stroke Registry France. The present study is a retrospective analysis 4029 stroke patients with treated between January 2015 May 2020 18 centers. systematically collected procedural data, incidence...