A5021094952- Acute Ischemic Stroke Management
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- Protease and Inhibitor Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Photoacoustic and Ultrasonic Imaging
- Traumatic Brain Injury and Neurovascular Disturbances
- Ultrasound Imaging and Elastography
- Blood Coagulation and Thrombosis Mechanisms
- Ultrasound and Hyperthermia Applications
- Neuroscience and Neuropharmacology Research
- Intracranial Aneurysms: Treatment and Complications
- Memory and Neural Mechanisms
- Moyamoya disease diagnosis and treatment
- Intracerebral and Subarachnoid Hemorrhage Research
- Cell Image Analysis Techniques
- Advanced MRI Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Neurological Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Sirtuins and Resveratrol in Medicine
- Neurosurgical Procedures and Complications
- Cancer, Hypoxia, and Metabolism
Inserm
2015-2024
Université de Caen Normandie
2015-2024
Cyceron
2015-2024
Normandie Université
2016-2024
Physiopathologie et imagerie des troubles neurologiques
2017-2024
Établissement Français du Sang
2022-2024
Hypertension pulmonaire : physiopathologie et innovation thérapeutique
2019-2022
Centre Hospitalier Universitaire de Caen
2017-2021
Vall d'Hebron Institut de Recerca
2021
Universitat Autònoma de Barcelona
2011-2021
Numerous treatments have been reported to provide a beneficial outcome in experimental animal stroke models; however, these (with the exception of tissue plasminogen activator) failed clinical trials. To improve translation treatment efficacy from bench bedside, we performed preclinical randomized controlled multicenter trial (pRCT) test potential therapy under circumstances closer design and rigor control trial. Anti-CD49d antibodies, which inhibit migration leukocytes into brain, were...
Recanalization is the mainstay of ischemic stroke treatment. However, even with timely clot removal, many patients recover poorly. Leptomeningeal collaterals (LMCs) are pial anastomotic vessels yet-unknown functions. We applied laser speckle imaging, ultrafast ultrasound, and two-photon microscopy in a thrombin-based mouse model fibrinolytic treatment to show that LMCs maintain cerebral autoregulation allow for gradual reperfusion, resulting small infarcts. In mice poor LMCs, distal arterial...
Early reperfusion using tissue-type plasminogen activator is the only therapeutic agent to treat focal cerebral ischemia with proven efficacy in patients. Nevertheless, novel insights into pathophysiology of neurons, glial cells, and fate endothelium after stroke call for use new strategies improve treatment alone or combination activator-induced thrombolysis. Unfortunately, despite plethora drugs that display clear beneficial effects animal models experimental ischemia, their subsequent...
Hyperfibrinolysis is a systemic condition occurring in various clinical disorders such as trauma, liver cirrhosis, and leukemia. Apart from increased bleeding tendency, the pathophysiological consequences of hyperfibrinolysis remain largely unknown. Our aim was to develop an experimental model study its effects on homeostasis blood-brain barrier (BBB). We induced sustained hyperfibrinolytic state mice by hydrodynamic transfection plasmid encoding for tissue-type plasminogen activator (tPA)....
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Objective:</i> Ultrasound Localization Microscopy (ULM) provides images of the microcirculation in-depth in living tissue. However, its implementation two-dimension is limited by elevation projection and tedious plane-by-plane acquisition. Volumetric ULM alleviates these issues can map vasculature entire organs one acquisition with isotropic resolution. optimal requires many independent...
Neuroglia critically shape the brain´s response to ischemic stroke. However, their phenotypic heterogeneity impedes a holistic understanding of cellular composition early lesion. Here we present single cell resolution transcriptomics dataset acute infarction. Oligodendrocyte lineage cells and astrocytes range among most transcriptionally perturbed populations exhibit infarction- subtype-specific molecular signatures. Specifically, find infarction restricted proliferating oligodendrocyte...
NAD+ depletion is a common phenomenon in neurodegenerative pathologies. Excitotoxicity occurs multiple neurologic disorders and was shown to prevent neuronal degeneration this process through mechanisms that remained be determined. The activity of nicotinamide riboside (NR) neuroprotective models the recent description extracellular conversion NR prompted us probe effects protection against excitotoxicity. Here, we show intracortical administration but not reduces brain damage induced by...
Stroke induces a multiphasic systemic immune response, but the consequences of this response on atherosclerosis-a major source recurrent vascular events-have not been thoroughly investigated. We show that stroke exacerbates atheroprogression via alarmin-mediated propagation inflammation. The prototypic brain-released alarmin high-mobility group box 1 protein induced monocyte and endothelial activation receptor for advanced glycation end products (RAGE)-signaling cascade increased plaque load...
Stroke represents a global challenge and is leading cause of permanent disability worldwide. Despite much effort, translation research findings to clinical benefit has not yet been successful. Failure neuroprotection trials considered, in part, due the low quality preclinical studies, level reproducibility across different laboratories that stroke co-morbidities have fully considered experimental models. More rigorous testing new drug candidates models initiation cross-laboratory studies...
<h3>Objective</h3> To validate a panel of blood biomarkers to differentiate between ischemic stroke (IS) and intracerebral hemorrhage (ICH) in patients with suspected stroke. <h3>Methods</h3> Patients admitted within 4.5 hours after onset were enrolled. Blood samples collected at hospital admission. Glial fibrillary acid protein (GFAP), retinol binding 4 (RBP-4), N-terminal proB-type natriuretic peptide (NT-proBNP), endostatin measured by immunoassays. Cutoff points obtained for 100%...
Early diagnosis is a critical part of the emergency care cerebral hemorrhages and ischemia. A rapid accurate strokes reduces delays to appropriate treatments better functional recovery. Currently, CTscan MRI are gold standards with constraints accessibility, availability, possibly some contraindications. The development Ultrasound Localization Microscopy (ULM) has enabled new perspectives conventional transcranial ultrasound imaging increased sensitivity, penetration depth, resolution....
Tissue-type plasminogen activator (tPA) is the only drug approved for acute treatment of ischemic stroke but with two faces in disease: beneficial fibrinolysis vasculature and damaging effects on neurovascular unit brain parenchyma. To improve this profile, we developed a novel strategy, relying antibodies targeting proneurotoxic tPA.After production characterization (αATD-NR1) that specifically prevent interaction tPA ATD-NR1 N-methyl-d-aspartate receptors, have evaluated their efficacy...
In the field of ischemic cerebral injury, precise characterization neurovascular hemodynamic is required to select candidates for reperfusion treatments.It thus admitted that advanced imaging-based approaches would be able better diagnose and prognose those patients contribute clinical care.Current imaging modalities like MRI allow a diagnostic injury but suffer from limited availability transportability.The recently developed ultrafast ultrasound could powerful tool perform emergency long...
Abstract The pharmacological intervention for ischemic stroke hinges on intravenous administration of the recombinant tissue-type plasminogen activator (rtPA, Alteplase/Actilyse) either as a standalone treatment or in conjunction with thrombectomy. However, despite its clinical significance, broader use rtPA is constrained because risk hemorrhagic transformations (HTs). Furthermore, presence diabetes chronic hyperglycemia associated an elevated HT subsequent to thrombolysis. This detrimental...
Cerebral circulation ensures the proper functioning of entire human body, and its interruption, i.e. stroke, leads to irreversible damage. However, tools for observing cerebral are still lacking. Although MRI CT scans serve as conventional methods, their accessibility remains a challenge, prompting exploration into alternative, portable, non-ionizing imaging solutions like ultrasound with reduced costs. While Ultrasound Localization Microscopy (ULM) displays potential in high-resolution...
The fibrinolytic and matrix metalloproteinase (MMP) systems cooperate in thrombus dissolution extracellular proteolysis. plasminogen/plasmin system activates MMPs, some MMPs have been involved the of fibrin by targeting fibrin(ogen) directly or collaborating with plasmin. MMP-10 has implicated inflammatory/thrombotic processes vascular integrity, but whether could a profibrinolytic effect represent promising thrombolytic agent is unknown.The on fibrinolysis was studied vitro vivo,...
See Sun et al. (doi:10.1093/aww306) for a scientific commentary on this article. About 20% of patients with ischaemic stroke have preceding transient attack, which is clinically defined as focal neurological symptoms origin resolving spontaneously. Failure to diagnose attack wasted opportunity prevent recurrent disabling stroke. Unfortunately, diagnosis can be difficult, due numerous mimics, and the absence specific test. New diagnostic tools are thus needed, in particular radiologically...
Background Intracranial aneurysms may be associated with an underlying arteriopathy, leading to arterial wall fragility. Arterial tortuosity is a major characteristic of some connective tissue disease. Aim To determine whether intracranial aneurysm arteriopathy. Methods Using case-control design, from May 2012 2013, we selected cases and controls consecutive patients who had conventional cerebral angiography in our center. Cases were newly diagnosed aneurysm. Controls diagnostic free The...
Desmoteplase, a recombinant form of the plasminogen activator DSPAalpha1 from Desmodus rotundus, may offer improved clinical benefits for acute ischemic stroke treatment over current therapy, tissue (rtPA). Accumulating evidence suggests that use rtPA could be limited by unfavorable properties, including its ability to cross blood-brain barrier (BBB), thus potentially adding pro-excitotoxic effect endogenous tPA in cerebral parenchyma. Here, investigate whether desmoteplase display safer...