A5055156298- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Ischemic Stroke Management
- S100 Proteins and Annexins
- Neurological Disease Mechanisms and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- Barrier Structure and Function Studies
- Bioinformatics and Genomic Networks
- Metabolomics and Mass Spectrometry Studies
- Cerebrovascular and Carotid Artery Diseases
- Advanced biosensing and bioanalysis techniques
- Long-Term Effects of COVID-19
- Blood Coagulation and Thrombosis Mechanisms
- Inflammasome and immune disorders
- interferon and immune responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Proteomics Techniques and Applications
- Chemokine receptors and signaling
- Computational Drug Discovery Methods
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Immune responses and vaccinations
- Apelin-related biomedical research
- Animal testing and alternatives
- Cardiac Fibrosis and Remodeling
LMU Klinikum
2021-2025
Ludwig-Maximilians-Universität München
2021-2025
Institut d'Investigacions Biomèdiques de Barcelona
2024
Consejo Superior de Investigaciones Científicas
2024
Vall d'Hebron Institut de Recerca
2014-2023
Universitat Autònoma de Barcelona
2014-2023
Inserm
2021
Cyceron
2021
Centre Hospitalier Universitaire de Caen
2021
Physiopathologie et imagerie des troubles neurologiques
2021
The bone marrow in the skull is important for shaping immune responses brain and meninges, but its molecular makeup among bones relevance human diseases remain unclear. Here, we show that mouse has most distinct transcriptomic profile compared with other states of health injury, characterized by a late-stage neutrophil phenotype. In humans, proteome analysis reveals distinct, differentially expressed neutrophil-related pathways unique synaptic protein signature. 3D imaging demonstrates...
The medical burden of stroke extends beyond the brain injury itself and is largely determined by chronic comorbidities that develop secondarily. We hypothesized these might share a common immunological cause, yet effects post-stroke on systemic immunity are underexplored. Here, we identify myeloid innate immune memory as cause remote organ dysfunction after stroke. Single-cell sequencing revealed persistent pro-inflammatory changes in monocytes/macrophages multiple organs up to 3 months...
Efficient and accurate nanocarrier development for targeted drug delivery is hindered by a lack of methods to analyze its cell-level biodistribution across whole organisms. Here we present Single Cell Precision Nanocarrier Identification (SCP-Nano), an integrated experimental deep learning pipeline comprehensively quantify the targeting nanocarriers throughout mouse body at single-cell resolution. SCP-Nano reveals tissue distribution patterns lipid nanoparticles (LNPs) after different...
Although the beneficial effects of statins on stroke have been widely demonstrated both in experimental studies and clinical trials, aim this study is to prepare characterize a new liposomal delivery system that encapsulates simvastatin improve its into brain.In order select optimal liposome lipid composition with highest capacity reach brain, male Wistar rats were submitted sham or transitory middle cerebral arterial occlusion (MCAOt) surgery treated (intravenous [IV]) fluorescent-labeled...
<h3>Objective</h3> To validate a panel of blood biomarkers to differentiate between ischemic stroke (IS) and intracerebral hemorrhage (ICH) in patients with suspected stroke. <h3>Methods</h3> Patients admitted within 4.5 hours after onset were enrolled. Blood samples collected at hospital admission. Glial fibrillary acid protein (GFAP), retinol binding 4 (RBP-4), N-terminal proB-type natriuretic peptide (NT-proBNP), endostatin measured by immunoassays. Cutoff points obtained for 100%...
Neuroinflammation after stroke is characterized by the activation of resident microglia and invasion circulating leukocytes into brain. Although lymphocytes infiltrate brain in small number, they have been consistently demonstrated to be most potent leukocyte subpopulation contributing secondary inflammatory injury. However, exact mechanism how this minimal number can profoundly affect outcome still largely elusive. Here, using a mouse model for ischemic stroke, we that early response...
Abstract The importance of the brain–heart interaction has been increasingly recognized as a critical physiological axis that is altered in disease. In this review, we explore intricate relationship between central nervous system and cardiovascular health, focusing particularly on immunological mechanisms influence course both neurological diseases. While previous studies have established key role autonomic (ANS) linking brain heart, more recent expanded our understanding multifaceted...
COVID-19 is associated with diverse neurological abnormalities, but the underlying mechanisms are unclear. We hypothesized that microglia, resident immune cells of brain, centrally involved in this process. To study this, we developed an autopsy platform allowing integration molecular anatomy, protein and mRNA datasets postmortem mirror blocks brain peripheral organ samples from cases COVID-19. observed focal loss microglial P2Y12R, CX3CR1-CX3CL1 axis deficits metabolic failure at sites...
We aimed to analyze ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) in relation arterial recanalization patients treated IV tissue plasminogen activator (tPA) futile mechanical thrombectomy.Acute ischemic stroke (n = 108) documented occlusions IV-tPA were selected. activity was measured by ELISA samples collected before treatment. Recanalization assessed at 2 hours transcranial Doppler. In 78 consecutive endovascular thrombectomy, antigen defined...
Abstract Background CCL 23 role in the inflammatory response after acute brain injuries remains elusive. Here, we evaluated whether blood levels associate with acquired cerebral lesions and determined predictive capacity for assessing stroke prognosis. We used preclinical models to study homologous chemokines rodents, 9 6. Methods Baseline were on 245 individuals, including ischaemic strokes ( IS ), mimics controls. Temporal profile of circulating was explored from baseline 24 h 20 . In an...
CCL23 is a chemokine implicated in inflammation and host defense responses. It has been recently associated with acquired brain damage stroke outcomes. In this study, we reported the role of Alzheimer’s disease (AD). We evaluated levels 659 individuals: cognitively normal, mild cognitive impaired (MCI), AD patients. Two cross-sectional (study 1, n = 53; study 2, 200) two longitudinal 3, 74; 4, 332) studies were analyzed separately. blood and/or cerebrospinal fluid (CSF) each measured by...
Cerebral ischemia entails rapid tissue damage in the affected brain area causing devastating neurological dysfunction. How each component of neurovascular unit contributes or responds to ischemic insult context human has not been solved yet. Thus, analysis proteome is a straightforward approach unraveling these cell proteotypes. In this study, post-mortem slices from stroke patients were obtained corresponding infarcted (IC) and contralateral (CL) areas. By means laser microdissection,...
Stroke remains a leading cause of death and disability worldwide. Despite continuous advances, the identification key molecular signatures in hyper-acute phase ischemic stroke is still primary interest for translational research on diagnosis, prognosis, treatment. Data integration from high-throughput -
Abstract Ischemic stroke is a major cause of death and disability worldwide. Translation into the clinical setting neuroprotective agents showing promising results in pre-clinical studies has systematically failed. One possible explanation that animal models used to test neuroprotectants do not properly represent population affected by stroke, as most are performed healthy young male mice. Therefore, we aimed determine if response cerebral ischemia differed depending on age, sex presence...